A fast, simple method for screening radiation susceptibility genes by RNA interference

Biochemical and Biophysical Research Communications

Volumn 333, Issue 4, 2005, Pages 1370-1377

  Tsuji, Atsushi B ^a   Sudo, Hitomi ^a   Sugyo, Aya ^a   Otsuki, Marika ^a   Miyagishi, Makoto ^b,d   Taira, Kazunari ^c,d   Imai, Takashi ^a   Harada, Yoshi Nobu ^a  

^a NATIONAL INSTITUTE OF RADIOLOGICAL SCIENCES   (Japan)

^b UNIVERSITY OF TOKYO   (Japan)

^c UNIVERSITY OF TOKYO   (Japan)

^d NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

Author keywords

Cell cycle; DNA repair; Ionizing radiation; Radiotherapy; RNA interference; X rays

Indexed keywords

BROXURIDINE; CYCLIN DEPENDENT KINASE INHIBITOR 1; ISOPROTEIN; PROTEIN KINASE; PROTON TRANSPORTING ADENOSINE TRIPHOSPHATE SYNTHASE; REAGENT; RNA; SHORT HAIRPIN RNA; SULFORHODAMINE B;

ARTICLE; CELL PROLIFERATION; CONTROLLED STUDY; FEMALE; GENETIC ASSOCIATION; GENETIC IDENTIFICATION; GENETIC SCREENING; GENETIC SUSCEPTIBILITY; HUMAN; HUMAN CELL; PRIORITY JOURNAL; RADIATION EXPOSURE; RNA INTERFERENCE; RNA VECTOR;

CELL COUNT; CELL SURVIVAL; DISEASE SUSCEPTIBILITY; DOSE-RESPONSE RELATIONSHIP, DRUG; GENE EXPRESSION PROFILING; GENE SILENCING; HELA CELLS; HUMANS; RADIATION DOSAGE; RADIATION INJURIES; RADIATION TOLERANCE; RADIATION, IONIZING; RISK ASSESSMENT; RISK FACTORS; RNA INTERFERENCE;

EID: 21344456958     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2005.06.047     Document Type: Article

References (25)

1. N.S. Russell, and A.C. Begg Editorial radiotherapy and oncology 2002: predictive assays for normal tissue damage Radiother. Oncol. 64 2002 125 129
2. C.N. Andreassen, J. Alsner, and J. Overgaard Does variability in normal tissue reactions after radiotherapy have a genetic basis-where and how to look for it? Radiother. Oncol. 64 2002 131 140
3. M. Iwakawa, T. Imai, Y. Harada, S. Ban, Y. Michikawa, K. Saegusa, M. Sagara, A. Tsuji, S. Noda, and A. Ishikawa RadGenomics project Nippon Igaku Hoshasen Gakkai Zasshi 62 2002 484 489
4. J.H. Hoeijmakers Genome maintenance mechanisms for preventing cancer Nature 411 2001 366 374
5. G. Iliakis, Y. Wang, J. Guan, and H. Wang DNA damage checkpoint control in cells exposed to ionizing radiation Oncogene 22 2003 5834 5847
6. B.G. Haffty, and P.M. Glazer Molecular markers in clinical radiation oncology Oncogene 22 2003 5915 5925
7. S.M. Elbashir, J. Harborth, W. Lendeckel, A. Yalcin, K. Weber, and T. Tuschl Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells Nature 411 2001 494 498
8. P.J. Paddison, J.M. Silva, D.S. Conklin, M. Schlabach, M. Li, S. Aruleba, V. Balija, A. O'Shaughnessy, L. Gnoj, K. Scobie, K. Chang, T. Westbrook, M. Cleary, R. Sachidanandam, W.R. McCombie, S.J. Elledge, and G.J. Hannon A resource for large-scale RNA-interference-based screens in mammals Nature 428 2004 427 431
9. K. Berns, E.M. Hijmans, J. Mullenders, T.R. Brummelkamp, A. Velds, M. Heimerikx, R.M. Kerkhoven, M. Madiredjo, W. Nijkamp, B. Weigelt, R. Agami, W. Ge, G. Cavet, P.S. Linsley, R.L. Beijersbergen, and R. Bernards A large-scale RNAi screen in human cells identifies new components of the p53 pathway Nature 428 2004 431 437
10. M. Miyagishi, and K. Taira Strategies for generation of an siRNA expression library directed against the human genome Oligonucleotides 13 2003 325 333
11. M. Miyagishi, S. Matsumoto, and K. Taira Generation of an shRNAi expression library against the whole human transcripts Virus Res. 102 2004 117 124
12. A.E. Carpenter, and D.M. Sabatini Systematic genome-wide screens of gene function Nat. Rev. Genet. 5 2004 11 22
13. B. Pauwels, A.E. Korst, C.M. de Pooter, G.G. Pattyn, H.A. Lambrechts, M.F. Baay, F. Lardon, and J.B. Vermorken Comparison of the sulforhodamine B assay and the clonogenic assay for in vitro chemoradiation studies Cancer Chemother. Pharmacol. 51 2003 221 226
14. J. Carmichael, W.G. DeGraff, A.F. Gazdar, J.D. Minna, and J.B. Mitchell Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of radiosensitivity Cancer Res. 47 1987 943 946
15. P. Price, and T.J. McMillan Use of the tetrazolium assay in measuring the response of human tumor cells to ionizing radiation Cancer Res. 50 1990 1392 1396
16. G. Griffon, J.L. Merlin, and C. Marchal Comparison of sulforhodamine B, tetrazolium and clonogenic assays for in vitro radiosensitivity testing in human ovarian cell lines Anticancer Drugs 6 1995 115 123
17. D. Banasiak, A.R. Barnetson, R.A. Odell, H. Mameghan, and P.J. Russell Comparison between the clonogenic, MTT, and SRB assays for determining radiosensitivity in a panel of human bladder cancer cell lines and a ureteral cell line Radiat. Oncol. Investig. 7 1999 77 85
18. A.R. Barnetson, D. Banasiak, R.J. Fisher, H. Mameghan, J.C. Ribeiro, K. Brown, J.L. Brown, S.M. O'Mara, and P.J. Russell Heterogeneity of in vitro radiosensitivity in human bladder cancer cells Radiat. Oncol. Investig. 7 1999 66 76
19. M. Miyagishi, and K. Taira U6 promoter-driven siRNAs with four uridine 3′ overhangs efficiently suppress targeted gene expression in mammalian cells Nat. Biotechnol. 20 2002 497 500
20. Y. Peng, Q. Zhang, H. Nagasawa, R. Okayasu, H.L. Liber, and J.S. Bedford Silencing expression of the catalytic subunit of DNA-dependent protein kinase by small interfering RNA sensitizes human cells for radiation-induced chromosome damage, cell killing, and mutation Cancer Res. 62 2002 6400 6404
21. S.L. Voytik-Harbin, A.O. Brightman, B. Waisner, C.H. Lamar, and S.F. Badylak Application and evaluation of the alamarBlue assay for cell growth and survival of fibroblasts In Vitro Cell. Dev. Biol. Anim. 34 1998 239 246
22. T. Porstmann, T. Ternynck, and S. Avrameas Quantitation of 5-bromo-2-deoxyuridine incorporation into DNA: an enzyme immunoassay for the assessment of the lymphoid cell proliferative response J. Immunol. Methods 82 1985 169 179
23. F. Bunz, A. Dutriaux, C. Lengauer, T. Waldman, S. Zhou, J.P. Brown, J.M. Sedivy, K.W. Kinzler, and B. Vogelstein Requirement for p53 and p21 to sustain G2 arrest after DNA damage Science 282 1998 1497 1501
24. W.L. Yan, T.J. Lerner, J.L. Haines, and J.F. Gusella Sequence analysis and mapping of a novel human mitochondrial ATP synthase subunit 9 cDNA (ATP5G3) Genomics 24 1994 375 377
25. G. Fang, H. Yu, and M.W. Kirschner Direct binding of CDC20 protein family members activates the anaphase-promoting complex in mitosis and G1 Mol. Cell 2 1998 163 171