Squalene epoxidase encoded by ERG1 affects morphogenesis and drug susceptibilities of Candida albicans

Journal of Antimicrobial Chemotherapy

Volumn 55, Issue 6, 2005, Pages 905-913

  Pasrija, Ritu ^a   Krishnamurthy, Shankarling ^b,c   Prasad, Tulika ^a   Ernst, Joachim F ^b   Prasad, Rajendra ^a  

^a JAWAHARLAL NEHRU UNIVERSITY   (India)

^b HEINRICH HEINE UNIVERSITY   (Germany)

^c UNIVERSITY OF MEDICINE AND DENTISTRY OF NEW JERSEY   (United States)

Author keywords

Cdr1p; Dimorphism; Drug resistance; Ergosterol; Hyphae; Regulable promoter

Indexed keywords

ABC TRANSPORTER; AMPHOTERICIN B; ANTIFUNGAL AGENT; CYCLOHEXIMIDE; CYSTEINE; ERG1 PROTEIN; ERGOSTEROL; FLUCONAZOLE; KETOCONAZOLE; METHIONINE; MUTANT PROTEIN; NYSTATIN; SQUALENE; SQUALENE MONOOXYGENASE; TERBINAFINE; UNCLASSIFIED DRUG;

ALLELE; ANTIBIOTIC SENSITIVITY; ARTICLE; CANDIDA ALBICANS; CONTROLLED STUDY; DIFFUSION; ENZYME ANALYSIS; FUNGUS HYPHAE; FUNGUS MUTANT; GENE DISRUPTION; GENE REPRESSION; HETEROZYGOSITY; LETHALITY; LIPID RAFT; MORPHOGENESIS; NONHUMAN; PROMOTER REGION; PROTEIN LOCALIZATION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; STEROL ANALYSIS; STEROL SYNTHESIS;

CANDIDA ALBICANS; DIFFUSION; ERGOSTEROL; FUNGAL PROTEINS; MEMBRANE TRANSPORT PROTEINS; MORPHOGENESIS; MUTATION; OXYGENASES; SQUALENE MONOOXYGENASE;

EID: 21244486724     PISSN: 03057453     EISSN: None     Source Type: Journal    
DOI: 10.1093/jac/dki112     Document Type: Article

References (37)

1. Lopez-Ribot, J. L., McAtee, R. K., Perea, S. et al. (1999). Multiple resistant phenotypes of Candida albicans coexist during episodes of oropharyngeal candidiasis in human immunodeficiency virus-infected patients. Antimicrob Agents Chemother 43, 1621-30.
2. Prasad, R., Panwar, S. L. & Krishnamurthy, S. (2002). Drug resistance mechanisms of human pathogenic fungi. In Fungal Pathogenesis: Principles and Clinical Applications (Calderone, R. A. & Cihlar, R. L., Eds), pp. 601-31. Marcel Dekker Inc., New York, NY, USA.
3. Prasad, R., Panwar, S. L., Smriti, & Poole, R. K. (2002). Drug resistance in yeasts - an emerging scenario. Adv Microb Physiol 46, 155-201.
4. Sanglard, D. & Odds, F. C. (2002). Resistance of Candida species to antifungal agents: molecular mechanisms and clinical consequences. Lancet Infect Dis 2, 73-85.
5. Kaur, R. & Bachhawat, A. K. (1999). The yeast multidrug resistance pump, Pdr5p, confers reduced drug resistance in erg mutants of Saccharomyces cerevisiae. Microbiology 145, 809-18.
6. Kohli, A., Smriti, Mukhopadhyay, K. et al. (2002). In vitro low-level resistance to azoles in Candida albicans is associated with changes in membrane lipid fluidity and asymmetry. Antimicrob Agents Chemother 46, 1046-52.
7. Mukhopadhyay, K., Prasad, T., Saini, P. et al. (2004). Membrane sphingolipid-ergosterol interactions are important determinants of multidrug resistance in Candida albicans. Antimicrob Agents Chemother 48, 1778-87.
8. Loffler, J., Einsele, H., Hebart, H. et al. (2000). Phospholipid and sterol analysis of plasma membranes of azole-resistant Candida albicans strains. FEMS Microbiol Lett 185, 59-63.
9. Hitchcock, C. A., Barrett-Bee, K. & Russel, N. J. (1986). The lipid composition of azole-sensitive and azole-resistant strains of Candida albicans. J Gen Microbiol 132, 2421-31.
10. Hitchcock, C. A., Russel, N. J. & Barrett-Bee, K. J. (1987). Sterols in Candida albicans mutants resistant to polyene or azole antifungals, and of a double mutant C. albicans 6.4. CRC Crit Rev Microbio1 15, 111-5.
11. Kelly, S. L., Lamb, D. C., Kelly, D. E. et al. (1996). Resistance to fluconazole and amphotericin B in Candida albicans from AIDS patients. Lancet 348, 1523-4.
12. Smriti, Krishnamurthy, S. & Prasad, R. (1999). Membrane fluidity affects functions of Cdr1p, a multidrug ABC transporter of Candida albicans. FEMS Microbiol Lett 173, 475-81.
13. Klobucnikova, V., Kohut, P., Leber, R. et al. (2003). Terbinafine resistance in a pleiotropic yeast mutant is caused by a single point mutation in the ERG1 gene. Biochem Biophys Res Commun 309, 666-71.
14. Sanglard, D., Ischer, F., Parkinson, T. et al. (2003). Candida albicans mutations in the ergosterol biosynthetic pathway and resistance to several antifungal agents. Antimicrob Agents Chemother 47, 2404-12.
15. Barrett-Bee, K. & Dixon, G. (1995). Ergosterol biosynthesis inhibition: a target for antifungal agents. Acta Biochim Pol 42, 465-80.
16. Parks, L. W. & Casey, W. M. (1995). Physiological implications of sterol biosynthesis in yeast. Annu Rev Microbiol 49, 95-116.
17. Pergakes, K. L. J. P., Lees, N. D., Barbuch, R. et al. (1998). Sequencing, disruption and characterization of Candida albicans sterol methyltransferase (ERG6) gene: drug susceptibility studies in erg6 mutants. Antimicrob Agents Chemother 42, 1160-7.
18. Jia, N., Arthington Skaggs, B., Lee, W. et al. (2002). C. albicans sterol C-14 reductase, encoded by the ERG24 gene, as a potential target site. Antimicrob Agents Chemother 46, 947-57.
19. Aaron, K. E., Pierson, C. A., Lees, D. et al. (2001). The Candida albicans ERG26 gene encoding the C-3 sterol dehydrogenase (C-4 decarboxylase) is essential for growth. FEMS Yeast Res 1, 93-101.
20. Favre, G. & Ryder, N. S. (1997). Cloning and expression of squalene epoxidase from pathogenic yeast, Candida albicans. Gene 189, 119-26.
21. Leber, R., Fuchsbichler, S., Klobucnikova, V. et al. (2003). Molecular mechanism of terbinafine resistance in S. cerevisiae. Antimicrob Agents Chemother 47, 3890-900.
22. Tsai, H. F., Bard, M., Izumikawa, K. et al. (2004). Candida glabrata mutant with increased sensitivity to azoles and to low oxygen tension. Antimicrob Agents Chemother 48, 2483-9.
23. Leber, R., Landl, K., Zinser, E. et al. (1998). Dual localization of squalene epoxidase, Erg1p, in yeast reflects a relationship between the endoplasmic reticulum and lipid particles. Mol Biol Cell 9, 375-86.
24. Care, R. S., Trevethick, J., Binley, K. M. et al. (1999). The MET3 promoter: a new tool for C. albicans molecular genetics. Mol Microbiol 34, 792-8.
25. Krishnamurthy, S., Plaine, A., Albert, J. et al. (2004). Dosage-dependent functions of fatty acid desaturase Ole1p in growth and morphogenesis of Candida albicans. Microbiology 150, 1991-2003.
26. Fonzi, W. A. & Irwin, M. Y. (1993). Isogenic strain construction and gene mapping in Candida albicans. Genetics 134, 717-28.
27. Alani, E., Cao, L. & Kleckner, N. (1987). A method for gene disruption that allows repeated use of URA3 selection in the construction of multiple disrupted yeast strains. Genetics 116, 541-5.
28. Reuss, O., Vik, A., Kolter, R. et al. (2004). The SAT1 flipper, an optimized tool for gene disruption in Candida albicans. Gene 341, 119-27.
29. Bocking, T., Barrow, K. D., Netting, A. G. et al. (2003). Effects of singlet oxygen on membrane sterols in the yeast Saccharomyces cerevisiae. Eur J Biochem 267, 1607-18.
30. Shukla, S., Saini, P., Smriti, et al. (2003). Functional characterization of Candida albicans ABC transporter Cdr1p. Eukaryotic Cell 2, 1361-75.
31. Timpel, C., Strahl-Bolsinger, S., Ziegelbauer, K. et al. (1998). Multiple functions of Pmt1p-mediated protein O-mannosylation in the fungal pathogen Candida albicans. J Biol Chem 273, 20837-46.
32. Mao, X., Hu, Y., Liang, C. et al. (2002). MET3 promoter: a tightly regulated promoter and its application in construction of conditional lethal strain. Curr Microbiol 45, 37-40.
33. Kelly, S. L., Lamb, D. C., Kelly, D. E. et al. (1997). Resistance to fluconazole and crossresistance to amphotericin B in Candida albicans from AIDS patients caused by detective sterol 5,6-desaturation. FEBS Lett 400, 80-2.
34. Sorger, D., Athenstaedth, K., Harstnik, C. et al. (2004). A yeast strain lacking lipid particles bears defect in ergosterol formation. J Biol Chem 279, 31190-6.
35. Lees, N. D., Broughton, M. C., Sanglard, D. et al. (1990). Azole susceptibility and hyphal formation in a cytochrome P-450 deficient mutant of Candida albicans. Antimicrob Agents Chemother 34, 831-6.
36. Shimokawa, O., Kato, Y. & Nakayama, H. (1986). Accumulation of 14-methyl sterols and defective hyphal growth in Candida albicans. J Med Vet Mycol 24, 327-36.
37. Martin, S. W. & Konopka, J. B. (2004). Lipid rafts polarization contributes to hyphal growth in Candida albicans. Eukaryotic Cell 3, 675-84.