The Candida albicans ERG26 gene encoding the C-3 sterol dehydrogenase (C-4 decarboxylase) is essential for growth

FEMS Yeast Research

Volumn 1, Issue 2, 2001, Pages 93-101

  Aaron, Kora E ^a   Pierson, Charles A ^a   Lees, N Douglas ^a   Bard, Martin ^a  

^a INDIANA UNIVERSITY   (United States)

Author keywords

Antifungal target; Candida albicans; ERG26; Ergosterol; Sterol biosynthesis; Sterol C 4 demethylation

Indexed keywords

BENZALKONIUM CHLORIDE; CARBOXYLYASE; COMPLEMENT COMPONENT C3; OXIDOREDUCTASE; STEROL DERIVATIVE; 4ALPHA CARBOXYSTEROL C3 DEHYDROGENASE; 4ALPHA-CARBOXYSTEROL-C3 DEHYDROGENASE; FUNGAL PROTEIN; STEROL;

ARTICLE; CANDIDA ALBICANS; CELL GROWTH; CONTROLLED STUDY; FUNGAL GENE; FUNGAL STRAIN; FUNGUS IDENTIFICATION; GENE CASSETTE; GENE LIBRARY; GENETIC CODE; MOLECULAR CLONING; MUTANT; NONHUMAN; PROMOTER REGION; SACCHAROMYCES CEREVISIAE; SEQUENCE ANALYSIS; AMINO ACID SEQUENCE; BIOSYNTHESIS; DNA SEQUENCE; ENZYMOLOGY; ESSENTIAL GENE; GENE DELETION; GENE EXPRESSION REGULATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE;

CANDIDA; CANDIDA ALBICANS; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

AMINO ACID SEQUENCE; BASE SEQUENCE; CANDIDA ALBICANS; CARBOXY-LYASES; CLONING, MOLECULAR; FUNGAL PROTEINS; GENE DELETION; GENE EXPRESSION REGULATION, FUNGAL; GENES, ESSENTIAL; MOLECULAR SEQUENCE DATA; SEQUENCE ANALYSIS, DNA; STEROLS;

EID: 0035735154     PISSN: 15671356     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1567-1356(01)00017-4     Document Type: Article

References (29)

1. Clinical, cellular, and molecular factors that contribute to antifungal drug resistance
2. Anti-Candida drugs: The biochemical basis for their activity
3. The presence of an R467K amino acid substitution and loss of allelic variation correlates with an azole-resistant lanosterol 14-alpha-demethylase in Candida albicans
4. Genetic analysis of azole resistance in the Darlington strain of Candida albicans
5. Increased mRNA levels of ERG16, CDR, and MDR1 correlate with increases in azole resistance in Candida albicans isolates from a patient infected with human immunodeficiency virus
6. Molecular cloning and characterization of a novel gene of Candida albicans, CDR1, conferring multiple resistance to drugs and antifungals
7. Cloning of Candida albicans genes conferring resistance to azole antifungal agents: Characterization of CDR2, a new multidrug ABC transporter gene
8. A novel multidrug efflux transporter gene of the major facilitator superfamily from Candida albicans (FLU1) conferring resistance to fluconazole
9. Biochemistry and molecular biology of sterol synthesis in Saccharomyces cerevisiae
10. Characterization of the Saccharomyces cerevisiae ERG26 gene encoding the C-3 sterol dehydrogenase (C-4 decarboxylase) involved in sterol biosynthesis
11. Characterization of the Saccharomyces cerevisiae ERG27 gene encoding the 3-keto reductase involved in C-4 sterol demethylation
12. Cloning and characterization of ERG25, the Saccharomyces cerevisiae gene encoding the C-4 sterol methyl oxidase
13. A yeast sterol auxotroph (erg25) is rescued by addition of azole antifungals and reduced levels of heme
14. Gene isolation by complementation in Candida albicans and applications to physical and genetic mapping
15. Cloning and characterization of a Candida albicans maltase gene involved in sucrose utilization
16. Rapid hypothesis testing with Candida albicans through gene disruption with short homology regions
17. Biochemical and genetic characterization of Rbf1p, a putative transcription factor of Candida albicans
18. Stable transformation and regulated expression of an inducible reporter construct in Candida albicans using restriction enzyme-mediated integration
19. Transformation of intact yeast cells treated with alkali solutions
20. Susceptibilities of Candida albicans multidrug transporter mutants to various antifungal agents and other metabolic inhibitors
21. Improved method for high efficiency transformation of intact yeast cells
22. DNA sequencing with chain terminating inhibitors
23. Polyene resistance and the isolation of sterol mutants in Saccharomyces cerevisiae
24. Transfer RNA structural change is a key element in the reassignment of the CUG codon in Candida albicans
25. Sterol synthesis and viability of erg11 (cytochrome P-450 lanosterol demethylase) mutants in Saccharomyces cerevisiae and Candida albicans
26. Isolation of hem3 mutants from Candida albicans by sequential disruptions
27. Cloning, sequencing and disruption of the gene encoding sterol C-14 reductase in Saccharomyces cerevisiae
28. Evaluation of the CaMAL2 promoter for regulated expression of genes in Candida albicans
29. Chromosomal rearrangements associated with morphological mutants provide a means for genetic variation of Candida albicans