Histone methyltransferases G9a and GLP form heteromeric complexes and are both crucial for methylation of euchromatin at H3-K9

Genes and Development

Volumn 19, Issue 7, 2005, Pages 815-826

  Tachibana, Makoto ^a   Ueda, Jun ^a   Fukuda, Mikiko ^a   Takeda, Naoki ^b   Ohta, Tsutomu ^c   Iwanari, Hiroko ^d   Sakihama, Toshiko ^d   Kodama, Tatsuhiko ^d   Hamakubo, Takao ^d   Shinkai, Yoichi ^a  

^a KYOTO UNIVERSITY   (Japan)

^b KUMAMOTO UNIVERSITY   (Japan)

^c NATIONAL CANCER CENTER RESEARCH INSTITUTE   (Japan)

^d UNIVERSITY OF TOKYO   (Japan)

Author keywords

G9a; GLP; Histone; Methylation

Indexed keywords

HISTONE H3; HISTONE METHYLTRANSFERASE G9A; METHYLTRANSFERASE; PROTEIN GLP; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; EMBRYO; EMBRYO DEATH; EMBRYO DEVELOPMENT; EMBRYONIC STEM CELL; EUCHROMATIN; GENE SILENCING; GENETIC EPIGENESIS; GENETIC MARKER; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEFICIENCY; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN METHYLATION; PROTEIN PROTEIN INTERACTION; TRANSCRIPTION REGULATION;

ANIMALS; EUCHROMATIN; HISTONE-LYSINE N-METHYLTRANSFERASE; HISTONES; LYSINE; METHYLATION; MICE; PROTEIN STRUCTURE, QUATERNARY; STEM CELLS;

EID: 20144388930     PISSN: 08909369     EISSN: None     Source Type: Journal    
DOI: 10.1101/gad.1284005     Document Type: Article

References (40)

1. Aagaard, L., Laible, G., Selenko, P., Schmid, M., Dorn, R., Schotta, G., Kuhfittig, S., Wolf, A., Lebersorger, A., Singh, P.E., et al. 1999. Functional mammalian homologs of the Drosophila PEV-modifier Su(var)3-9 encode centromere-associated proteins which complex with the heterochromatin component M31. EMBO J. 18: 1923-1938.
2. Bannister, A.J., Zegerman, P., Partridge, J.F., Miska, E.A., Thomas, J.O., Allshire, R.C., and Kouzarides, T. 2001. Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain. Nature 410: 120-124.
3. Cao, R. and Zhang, Y. 2004a. The functions of E(Z)/EZH2-mediated methylation of lysine 27 in histone H3. Curr. Opin. Genet. Dev. 14: 155-164.
4. -. 2004b. SUZ12 is required for both the histone methyltransferase activity and the silencing function of the EED-EZH2 complex. Mol. Cell 15: 57-67.
5. Cheutin, T., McNairn, AJ., Jenuwein, T., Gilbert, D.M., Singh, P.B., and Misteli, T. 2003. Maintenance of stable heterochromatin domains by dynamic HP1 binding. Science 299: 721-725.
6. Dodge, J.E., Kang, Y.K., Beppu, H., Lei, H., and Li, E. 2004. Histone H3-K9 methyltransferase ESET is essential for early development. Mol. Cell. Biol. 24: 2478-2486.
7. Eissenberg, J.C. and Elgin, S.C. 2000. The HP1 protein family: Getting a grip on chromatin. Curr. Opin. Genet. Dev. 10: 204-210.
8. Eskeland, R., Czermin, B., Boeke, J., Bonaldi, T., Regula, J.T., and Imhof, A. 2004. The N-terminus of Drosophila SU(VAR)3-9 mediates dimerization and regulates its methyltransferase activity. Biochemistry 43: 3740-3749.
9. Fang, J., Feng, Q., Ketel, C.S., Wang, H., Cao, R., Xia, L., Erdjument-Bromage, H., Tempst, P., Simon, J.A., and Zhang, Y. 2002. Purification and functional characterization of SETS, a nucleosomal histone H4-lysine 20-specific methyltransferase. Curr. Biol. 12: 1086-1099.
10. Festenstein, R., Pagakis, S.N., Hiragami, K., Lyon, D., Verreault, A., Sekkali, B., and Kioussis, V. 2003. Modulation of heterochromatin protein 1 dynamics in primary mammalian cells. Science 299: 719-721.
11. Fischle, W., Wang, Y., Jacobs, S.A., Kim, Y., Allis, C.D., and Khorasanizadeh, S. 2003. Molecular basis for the discrimination of repressive methyl-lysinc marks in histone H3 by Polycomb and HP1 chromodomains. Genes & Dev. 17: 1870-1881.
12. Gyory, L, Wu, J., Fejer, G., Seto, E., and Wright, K.L. 2004. PRDI-BF1 recruits the histone H3 methyltransferase G9a in transcriptional silencing. Nat. Immunol. 5: 299-308.
13. Jenuwein, T. and Allis, C.D. 2001. Translating the histone code. Science 293: 1074-1080.
14. Lachner, M. and Jenuwein, T. 2002. The many faces of histone lysine methylation. Curr. Opin. Cell Biol. 14: 286-298.
15. Lachner, M., O'Carroll, D., Rea, S., Mechtler, K., and Jenuwein, T. 2001. Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins. Nature 410: 116-120.
16. Lachner, M., O'Sullivan, R.J., and Jenuwein, T. 2003. An epigenetic road map for histone lysine methylation. J. Cell Sci. 116: 2117-2124.
17. Luger, K., Mader, A.W., Richmond, R.K., Sargent, D.F., and Richmond, T.J. 1997. Crystal structure of the nucleosome core particle at 2.8 A resolution. Nature 389: 251-260.
18. Manzur, K.L., Farooq, A., Zeng, L., Plotnikova, O., Koch, A.W., Sachchidanand, and Zhou, M.M. 2003. A dimeric viral SET domain methyltransferase specific to Lys27 of histone H3. Nat. Struct. Biol. 10: 187-196.
19. Nishio, H. and Walsh, M.J. 2004. CCAAT displacement protein/cut homolog recruits G9a histone lysine methyltransferase to repress transcription. Proc. Natl. Acad. Sci. 101: 11257-11262.
20. Nishioka, K., Rice, J.C., Sarma, K., Erdjument-Bromage, H., Werner, J., Wang, Y., Chuikov, S., Valenzuela, P., Tempst, P., Steward, R., et al. 2002. PR-Set7 is a nucleosome-specific methyltransferase that modifies lysine 20 of histone H4 and is associated with silent chromatin. Mol. Cell 9: 1201-1213.
21. O'Carroll, D., Scherthan, H., Peters, A.H., Opravil, S., Haynes, A.R., Laible, G., Rea, S., Schmid, M., Lebersorger, A., Jerratsch, M., et al. 2000. Isolation and characterization of Suv39h2, a second histone H3 methyltransferase gene that displays testis-specific expression. Mol. Cell. Biol. 20: 9423-9433.
22. Ogawa, H., Ishiguro, K., Gaubatz, S., Livingston, D.M., and Nakatani, Y. 2002. A complex with chromatin modifiers that occupies E2F- and Myc-responsive genes in G0 cells. Science 296: 1132-1136.
23. Osipovich, O., Milley, R., Meade, A., Tachibana, M., Shinkai, Y., Krangel, M.S., and Oltz, E.M. 2004. Targeted inhibition of V(D)J recombination by a histone methyltransferase. Nat. Immunol. 5: 309-316.
24. Peters, A.H., O'Carroll, D., Scherthan, H., Mechtler, K., Sauer, S., Schofer, C., Weipoltshammer, K., Pagani, M., Lachner, M., Kohlmaier, A., et al. 2001. Loss of the Suv39h histone methyltransferases impairs mammalian heterochromatin and genome stability. Cell 107:323-337.
25. Peters, A.M., Kubicek, S., Mechtler, K., O'Sullivan, R.J., Derijck, A.A., Perez-Burgos, L., Kohlmaier, A., Opravil, S., Tachibana, M., Shinkai, Y., et al. 2003. Partitioning and plasticity of repressive histone methylation states in mammalian chromatin. Mol. Cell 12: 1577-1589.
26. Rea, S., Eisenhaber, F., O'Carroll, D., Strahl, B.D., Sun, Z.W., Schmid, M., Opravil, S., Mechtler, K., Ponting, C.P., Allis, C.D., et al. 2000. Regulation of chromatin structure by site-specific histone H3 methyltransferases. Nature 406: 593-599.
27. Rice, J.C., Briggs, S.D., Ueberheide, B., Barber, C.M., Shabanowitz, J., Hunt, D.F., Shinkai, Y., and Allis, C.D. 2003. Histone methyltransferases direct different degrees of methylation to define distinct chromatin domains. Mol. Cell 12: 1591-1598.
28. Roopra, A., Qazi, R., Schoenike, B., Daley, T.J., and Morrison, J.F. 2004. Localized domains of g9a-mediated histone methylation are required for silencing of neuronal genes. Mol. Cell 14: 727-738.
29. Sarraf, S.A. and Stancheva, I. 2004. Methyl-CpG binding protein MBD1 couples histone H3 methylation at lysine 9 by SETDB1 to DNA replication and chromatin assembly. Mol. Cell 15: 595-605.
30. Schotta, G., Lachner, M., Sarma, K., Ebert, A., Sengupta, R., Reuter, G., Reinberg, D., and Jenuwein, T. 2004. A silencing pathway to induce H3-K9 and H4-K20 trimethylation at constitutive heterochromatin. Genes & Dev. 18: 1251-1262.
31. Schultz, D.C., Ayyanathan, K., Negorev, D., Maul, G.G., and Rauscher III, F.J. 2002. SETDB1: A novel KAP-1-associated histone H3, lysine 9-specific methyltransferase that contributes to HP1-mediated silencing of euchromatic genes by KRAB zinc-finger proteins. Genes &. Dev. 16: 919-932.
32. Shi, Y., Sawada, J., Sui, G., Affarel, B., Whetstine, J.R., Lan, F., Ogawa, H., Luke, M.P., and Nakatani, Y. 2003. Coordinated histone modifications mediated by a CtBP co-repressor complex. Nature 422: 735-738.
33. Strahl, B.D. and Allis, C.D. 2000. The language of covalent histone modifications. Nature 403: 41-45.
34. Tachibana, M., Sugimoto, K., Fukushima, T., and Shinkai, Y. 2001. Set domain-containing protein, G9a, is a novel lysine-preferring mammalian histone methyltransferase with hyperactivity and specific selectivity to lysines 9 and 27 of histone H3. J. Biol. Chem. 276:25309-25317.
35. Tachibana, M., Sugimoto, K., Nozaki, M., Ueda, J., Ohta, T., Ohki, M., Fukuda, M., Takeda, N., Niida, H., Kato, H., et al. 2002. G9a histone methyltransferase plays a dominant role in euchromatic histone H3 lysine 9 methylation and is essential for early embryogenesis. Genes & Dev. 16: 1779-1791.
36. Tanaka, T., Takeno, T., Watanabe, Y., Uchiyama, Y., Murakami, T., Yamashita, H., Suzuki, A., Aoi, R., Iwanari, H., Jiang, S.Y., et al. 2002. The generation of monoclonal antibodies against human peroxisome proliferator-activated receptors (PPARs). J. Atheroscler. Thromb. 9: 233-242.
37. Turner, B.M. 2000. Histone acetylation and an epigenetic code. Bioessays 22: 836-845.
38. Wang, H., An, W., Cao, R., Xia, L., Erdjument-Bromage, H., Chatton, B., Tempst, P., Roeder, R.G., and Zhang, Y. 2003. mAM facilitates conversion by ESET of dimethyl to trimethyl lysine 9 of histone H3 to cause transcriptional repression. Mol. Cell 12: 475-487.
39. Yagi, T., Tokunaga, T., Furuta, Y., Nada, S., Yoshida, M., Tsukada, T., Saga, Y., Takeda, N., Ikawa, Y., and Aizawa, S. 1993. A novel ES cell line, TT2, with high germlinc-differentiating potency. Anal. Biochem. 214: 70-76.
40. Yang, L., Xia, L., Wu, D.Y., Wang, H., Chansky, H.A., Schubach, W.H., Hiekstein, D.D., and Zhang, Y. 2002. Molecular cloning of ESET, a novel histone H3-specific methyltransferase that interacts with ERG transcription factor. Oncogene 21: 148-152.