Kupffer cells and macrophages are not required for hepatic hepcidin activation during iron overload

Hepatology

Volumn 41, Issue 3, 2005, Pages 545-552

  Montosi, Giuliana ^a   Corradini, Elena ^a   Garuti, Cinzia ^a   Barelli, Samuele ^a   Recalcati, Stefania ^b   Cairo, Gaetano ^b   Valli, Linda ^a   Pignatti, Elisa ^a   Vecchi, Chiara ^a   Ferrara, Francesca ^a   Pietrangelo, Antonello ^a  

^a UNIVERSITY OF MODENA AND REGGIO EMILIA   (Italy)

^b UNIVERSITY OF MILAN   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

GADOLINIUM CHLORIDE; HEPCIDIN; IRON; IRON DEXTRAN; LIPOPOLYSACCHARIDE; MESSENGER RNA; TURPENTINE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BLOOD DONOR; CELL ACTIVATION; CONTROLLED STUDY; HEMOCHROMATOSIS; HEPATOMA CELL; HORMONE ACTION; HUMAN; HUMAN CELL; IN VITRO STUDY; KUPFFER CELL; MACROPHAGE; MALE; MOUSE; NONHUMAN; NORTHERN BLOTTING; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STEADY STATE; TOXICOLOGY;

ADULT; ANIMALS; ANTIMICROBIAL CATIONIC PEPTIDES; CELLS, CULTURED; FEMALE; GADOLINIUM; HISTOCOMPATIBILITY ANTIGENS CLASS I; HUMANS; IRON OVERLOAD; KUPFFER CELLS; LIPOPOLYSACCHARIDES; LIVER; MACROPHAGES; MALE; MEMBRANE PROTEINS; MICE; MICE, INBRED BALB C; MIDDLE AGED; RNA, MESSENGER;

EID: 20044366342     PISSN: 02709139     EISSN: None     Source Type: Journal    
DOI: 10.1002/hep.20620     Document Type: Article

References (29)

1. Krause A, Neitz S, Magert HJ, Schulz A, Forssmann WG, Schulz-Knappe P, et al. LEAP-1, a novel highly disulfide-bonded human peptide, exhibits antimicrobial activity. FEBS Lett 2000;480:147-150.
2. Park CH, Valore EV, Waring AJ, Ganz T. Hepcidin, a urinary antimicrobial peptide synthesized in the liver. J Biol Chem 2001;276:7806-7810.
3. Pigeon C, Ilyin G, Courselaud B, Leroyer P, Turlin B, Brissot P, Loreal O. A new mouse liver-specific gene, encoding a protein homologous to human antimicrobial peptide hepcidin, is overexpressed during iron overload. J Biol Chem 2001;276:7811-7819.
4. Nicolas G, Chauvet C, Viatte L, Danan JL, Bigard X, Devaux I, Beaumont C, et al. The gene encoding the iron regulatory peptide hepcidin is regulated by anemia, hypoxia, and inflammation. J Clin Invest 2002;110:1037-1044.
5. Bridle KR, Frazer DM, Wilkins SJ, Dixon JL, Purdie DM, Crawford DH, Subramaniam VN, et al. Disrupted hepcidin regulation in HFE-associated haemochromatosis and the liver as a regulator of body iron homoeostasis. Lancet 2003;361:669-673.
6. Gehrke SG, Kulaksiz H, Herrmann T, Riedel HD, Bents K, Veltkamp C, Stremmel W. Expression of hepcidin in hereditary hemochromatosis: evidence for a regulation in response to serum transferrin saturation and non-transferrin- bound iron. Blood 2003;102:371-376.
7. Nemeth E, Valore EV, Territo M, Schiller G, Lichtenstein A, Ganz T. Hepcidin, a putative mediator of anemia of inflammation, is a type II acute-phase protein. Blood 2003;101:2461-2463.
8. Nicolas G, Bennoun M, Devaux I, Beaumont C, Grandchamp B, Kahn A, Vaulont S. Lack of hepcidin gene expression and severe tissue iron overload in upstream stimulatory factor 2 (USF2) knockout mice. Proc Natl Acad Sci U S A 2001;98:8780-8785.
9. Courselaud B, Pigeon C, Inoue Y, Inoue J, Gonzalez FJ, Leroyer P, Gilot D, et al. C/EBPalpha regulates hepatic transcription of hepcidin, an antimicrobial peptide and regulator of iron metabolism. Cross-talk between C/EBP pathway and iron metabolism. J Biol Chem 2002;277:41163-41170.
10. Nicolas G, Bennoun M, Porteu A, Mativet S, Beaumont C, Grandchamp B, Sirito M, et al. Severe iron deficiency anemia in transgenic mice expressing liver hepcidin. Proc Natl Acad Sci U S A 2002;99:4596-4601.
11. Pietrangelo A. Hereditary hemochromatosis - a new look at an old disease. N Engl J Med 2004;350:2383-2397.
12. Nemeth E, Rivera S, Gabayan V, Keller C, Taudorf S, Pedersen BK, Ganz T. IL-6 mediates hypoferremia of inflammation by inducing the synthesis of the iron regulatory hormone hepcidin. J Clin Invest 2004;113:1271-1276.
13. Lee P, Peng H, Gelbart T, Beutler E. The IL-6- and lipopolysaccharide- induced transcription of hepcidin in HFE-, transferrin receptor 2-, and beta2-microglobulin-deficient hepatocytes. Proc Natl Acad Sci U S A 2004;101:9263-9265.
14. Roy CN, Custodio AO, de Graaf J, Schneider S, Akpan I, Montross LK, Sanchez M, et al. An Hfe-dependent pathway mediates hyposideremia in response to lipopolysaccharide-induced inflammation in mice. Nat Genet 2004;36:481-485.
15. Frazer DM, Wilkins SJ, Millard KN, McKie AT, Vulpe CD, Anderson GJ. Increased hepcidin expression and hypoferraemia associated with an acute phase response are not affected by inactivation of HFE. Br J Haematol 2004;126:434-436.
16. Ganz T. Hepcidin, a key regulator of iron metabolism and mediator of anemia of inflammation. Blood 2003;102:783-788.
17. Hardonk MJ, Dijkhuis FW, Hulstaert CE, Koudstaal J. Heterogeneity of rat liver and spleen macrophages in gadolinium chloride-induced elimination and repopulation. J Leukoc Biol 1992;52:296-302.
18. Lazar G, Jr., Farkas G, Csanadi J, Lazar G. Gadolinium chloride-induced macrophage blockade prevents rejection of human insulinoma cell xenograft in tats. Transplantation 1997;63:729-732.
19. Olynyk JK, Yeoh GC, Ramm GA, Clarke SL, Hall PM, Britton RS, Bacon BR, et al. Gadolinium chloride suppresses hepatic oval cell proliferation in rats with biliary obstruction. Am J Pathol 1998;152:347-352.
20. Montosi G, Paglia P, Garuti C, Guzman CA, Bastin JM, Colombo MP, Pietrangelo A. Wild-type HFE protein normalizes transferrin iron accumulation in macrophages from subjects with hereditary hemochromatosis. Blood 2000;96:1125-1129.
21. Berridge MV and Tan AS, Characterization of the cellular reduction of 3-(4,5-dimethylthiazol-2,5-diphenyltetrazolium bromide (MTT): subcellular localization, substrate dependence, and involvement of mitochondrial electron transport in MTT reduction, Arch Biochem Biophys 1993; 303:474-482.
22. Cleveland DW, Lopata MA, MacDonald RJ, Cowan NJ, Rutter WJ, Kirschner MW. Number and evolutionary conservation of alpha- and beta-tubulin and cytoplasmic beta- and gamma-actin genes using specific cloned cDNA probes. Cell 1980;20:95-105.
23. Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 2001;29-45.
24. Lee CM, Yeoh GC, Olynyk JK. Differential effects of gadolinium chloride on Kupffer cells in vivo and in vitro. Int J Biochem Cell Biol 2004;36:481-488.
25. Nemeth E. Tuttle M.S. Powelson J. Vaughn MB, Donovan A, Ward DM, Ganz T. et al. Hepcidin regulates iron efflux by binding to ferroportin and inducing its internalization. Science, in press.
26. Yang F, Liu XB, Quinones M, Melby PC, Ghio A. and Haile D J. Regulation of reticuloendothelial iron transporter MTP1 (Slc1 1a3) by inflammation. J Biol Chem 2002; 39786-91.
27. Papanikolaou G, Samuels ME, Ludwig EH, MacDonald ML, Franchini PL, Dube MP, Andres L, et al. Mutations in HFE2 cause iron overload in chromosome 1q-linked juvenile hemochromatosis. Nat Genet 2004;36:77-82.
28. Nemeth E, Roetto A, Garozzo G, Ganz T. and Camaschella, C. Hepcidin is decreased in TFR2-Hemochromatosis. Blood, in press.
29. Pietrangelo A, Caleffi A, Henrion J, Ferrara F, Corradini E, Kulaksiz H, Stremmel W, et al. Juvenile hemochromatosis associated with pathogenic mutations of adult hemochromatosis genes. Gastroenterology, in press.