Garlic arrests MDA-MB-435 cancer cells in mitosis, phosphorylates the proapoptotic BH3-only protein BimEL and induces apoptosis

British Journal of Cancer

Volumn 92, Issue 9, 2005, Pages 1773-1781

  Lund, T ^a   Stokke, T ^a   Olsen, O E ^a   Fodstad, O ^a  

^a OSLO UNIVERSITY HOSPITAL   (Norway)

Author keywords

Apoptosis; BimEL; Garlic; Mitosis

Indexed keywords

2 (2 AMINO 3 METHOXYPHENYL)CHROMONE; ANTHRA[1,9 CD]PYRAZOL 6(2H) ONE; BH3 PROTEIN; BIM PROTEIN; DNA; GARLIC EXTRACT; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 3; MITOGEN ACTIVATED PROTEIN KINASE INHIBITOR; PROTEIN BCL 2; STRESS ACTIVATED PROTEIN KINASE;

APOPTOSIS; ARTICLE; CANCER CELL; CELL CYCLE; ENZYME INHIBITION; FLOW CYTOMETRY; GARLIC; HUMAN; HUMAN CELL; MITOSIS; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION;

APOPTOSIS; APOPTOSIS REGULATORY PROTEINS; BH3 INTERACTING DOMAIN DEATH AGONIST PROTEIN; CARRIER PROTEINS; CELL LINE, TUMOR; GARLIC; HUMANS; MEMBRANE PROTEINS; MICROTUBULES; MITOSIS; PHOSPHORYLATION; PLANT EXTRACTS; PROTO-ONCOGENE PROTEINS;

EID: 19944394839     PISSN: 00070920     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.bjc.6602537     Document Type: Article

References (37)

1. Agarwal KC (1996) Therapeutic actions of garlic constituents. Med Res Rev 16: 111-124
2. Antisperger DSM, Dirsch VM, Ferreira D, Su J-L, Kuo M-L, Vollmar AM (2003) Ajoene-induced cell death in human promyeloleukemic cells does not require JNK but is amplified by the inhibition of ERK. Oncogene 22: 582-589
3. Biswas SC, Greene LA (2002) Nerve growth factor (NGF) down-regulates the Bcl-2 homology 3 (BH3) domain-only protein Bim and suppresses its proapoptotic activity by phosphorylation. J Biol Chem 277: 49511-49516
4. Bouillet P, Metcalf D, Huang DCS, Tarlinton DM, Kay TWH, Ksntgen F, Adams JM, Strasser A (1999) Proapoptotic Bcl-2 relative Bim required for certain apoptotic responses, leukocyte homeostasis, and to preclude autoimmunity. Science 286: 1735-1738
5. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254
6. EL triggers a positive feedback amplification of apoptotic signalling. Proc Natl Acad Sci USA 101: 1235-1240
7. Cory S, Adams JM (2002) The Bcl-2 family: regulators of the cellular life-or-death switch. Nat Rev Cancer 2: 647-656
8. Dirsch VM, Gerbes AL, Vollmar AM (1998) Ajoene, a compound of garlic, induces apoptosis in human promyeloleukemic cells, accompanied by generation of reactive oxygen species and activation of nuclear factor êB. Mol Pharmacol 53: 402-407
9. Dreskin SC, Thomas GW, Dale SN, Heasley LE (2001) Isoforms of Jun kinase are differentially expressed and activated in human monocyte/macrophage (THP-1) cells. J Immunol 166: 5646-5653
10. Ellison G, Klinowska T, Westwood RF, Docter E, French T, Fox JC (2002) Further evidence to support the melanocytic origin of MDA-MB-435. Mol Pathol 55: 294-299
11. Filomeni G, Aquilano K, Rotilio G, Ciriolo MR (2003) Reactive oxygen species-dependent c-jun NH2-terminal kinase/c-jun signalling cascade mediates neuroblastoma cell death induced by diallyl disulfide. Cancer Res 63: 5940-5949
12. Gavrielli Y, Sherman Y, Ben-Sasson SA (1992) Identification of programmed cell death in situ via specific labelling of nuclear DNA fragmentation. J Cell Biol 119: 493-501
13. Han J, Lawson L, Han G, Han P (1995) A spectrophotometric method for quantitative determination of allicin and total garlic thiosulfinates. Anal Biochem 225: 157-160
14. Huang DCS, Strasser A (2000) BH3-only proteins - essential initiators of apoptotic cell death. Cell 103: 839-842
15. 2/M phase arrest induced by diallyl disulfide in HCT-15 cells. Nutr Cancer 30: 169-174
16. cdc2 kinase activity through changes in complex formation and phosphorylation. Carcinogenesis 21: 1129-1134
17. 2 phase nuclei with mithramycin, propidium iodide, ethidium bromide after fixation with formaldehyde. Cytometry 7: 54-63
18. Lawson LD, Wang ZJ (1993) Pre-hepatic fate of the organosulfur compounds derived from garlic (Alllium sativum). Planta Med 59(Suppl): A688-A689
19. Lei K, Davis RJ (2003) JNK phosphorylation of Bim-related members of the Bcl-2 family induces Bax-dependent apoptosis. Proc Natl Acad Sci USA 100: 2432-2437
20. Ley R, Balmanno K, Hadfield K, Weston C, Cook SJ (2003) Activation of the ERK1/2 signaling pathway promotes phosphorylation and proteasome-dependent degradation of the BH3-only protein, Bim. J Biol Chem 278: 18811-18816
21. EL by ERK1/2 on serine 69 promotes its degradation via the proteasome pathway and regulates its proapoptotic function. Oncogene 22: 6785-6793
22. Marani M, Hancock D, Lopes R, Tenev T, Downward J, Lemoine NR (2004) Role of Bim in the survival pathway induced by Raf in epithelial cells. Oncogene 23: 2431-2441
23. Marani M, Tenev T, Hancock D, Downward J, Lemoine NR 2002)) Identification of novel isoforms of the BH3 domain protein Bim which directly activate bax to trigger apoptosis. Mol Cell Biol 22: 3577-3589
24. Nakagawa H, Tsuta K, Kiucki K, Senzaki H, Tanaka K, Tsubura A (2001) Growth inhibitory effects of diallyl disulfide on human breast cancer cell lines. Carcinogenesis 22: 891-897
25. O'Connor L, Strasser A, O'Reilly LA, Hausmann G, Adams JM, Cory S, Huang DCS (1998) Bim-a novel member of the Bcl-2 family that promotes apoptosis. EMBO J 17: 384-395
26. O'Connor PM, Jackman J, Bae I, Myers TG, Fan S, Mutoh M, Scudier DA, Monks A, Sausville EA, Weinstein JN, Friend S, Fornace Jr AJ, Kohn KW (1997) Characterization of the p53 tumor suppressor pathway in cell lines of the National Cancer Institute anticancer drug screen and correlations with the growth-inhibitory potency of 123 anticancer agents. Cancer Res 57: 4285-4300
27. O'Reilly LA, Cullen L, Visvader J, Lindeman GJ, Print C, Bath ML, Huang DCS, Strasser A (2000) The proapoptotic BH3-only protein Bim is expressed in hematopoietic, epithelial, neuronal, and germ cells. Am J Pathol 157: 449-461
28. Putcha GV, Le S, Frank S, Besirli CG, Clark K, Chu B, Alix S, Youle RJ, LaMarche A, Maroney AC, Johnson Jr EM (2003) JNK-mediated Bim phosphorylation potentiates BAX-dependent apoptosis. Neuron 38: 899-914
29. Puthalakath H, Huang DCS, O'Reilly LA, King SM, Strasser A (1999) The proapoptotic activity of the Bcl-2 family member Bim is regulated by interaction with the dynein motor complex. Mol Cell 3: 287-296
30. Seward RJ, von Haller PD, Aebersold R, Huber BT (2003) Phosphorylation of the pro-apoptotic protein Bim in lymphocytes is associated with protection from apoptosis. Mol Immunol 39: 983-993
31. Shirin H, Pinto JT, Kawabata Y, Soh J-W, Delohery T, Moss SF, Murty V, Rivlin RS, Holt PR, Weinstein IB (2001) Antiproliferative effects of S-allylmercaptocysteine on colon cancer cells when tested alone or in combination with sulindac sulphide. Cancer Res 61: 725-731
32. Sivam GP, Lampe JW, Ulness B, Swanzy SR, Potter JD (1997) Helicobacter pylori - in vitro susceptibility to garlic (Allium sativum) extract. Nutr Cancer 27: 118-121
33. Stokke T, Solberg K, DeAngelis P, Steen HB (1998) Propidium iodide quenches the fluorescence of TdT incorporated FITC-labeled dUTP in apoptotic cells. Cytometry 33: 428-434
34. Wang P, Gilmore AP, Streuli CH (2004) Bim is an apoptosis sensor that responds to loss of survival signals delivered by Epidermal Growth Factor but not those provided by integrins. J Biol Chem 279: 41280-41285
35. Xiao D, Choi S, Johnson DE, Vogel VG, Johnson CS, Trump DL, Lee YL, Singh SV (2004) Diallyl trisulfide-induced apoptosis in human prostate cancer cells involves c-Jun N-terminal kinase and extracellular-signal regulated kinase-mediated phosphorylation of Bcl-2. Oncogene 23: 5594-5606
36. Xiao D, Pinto JT, Soh J-W, Deguchi A, Gundersen GG, Palazzo AF, Yoon J-T, Shirin H, Weinstein BI (2003) Induction of apoptosis by the garlic-derived compound s-Allylmercaptocysteine (SAMC) is associated with microtubule depolymerization and c-jun NH2-terminal kinase 1 activation. Cancer Res 63: 6825-6837
37. Zelivianski S, Spellman M, Kellerman M, Kakitelashvilli V, Zhou XW, Lugo E, Lee MS, Taylor R, Davis TL, Hauke R, Lin MF (2003) ERK inhibitor PD98059 enhances docetaxel-induced apoptosis of androgen-independent human prostate cancer cells. Int J Cancer 107: 478-485