Mechanistic studies on aggregation of polyethylenimine-DNA complexes and its prevention

Biotechnology and Bioengineering

Volumn 90, Issue 5, 2005, Pages 614-620

  Sharma, Vikas K ^a   Thomas, Mini ^a   Klibanov, Alexander M ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

Aggregation; Nonviral gene delivery; Plasmid DNA; Polyethylenimine; Stabilization; Surfactants

Indexed keywords

COMPLEX CONCENTRATION; GENE DELIVERY; MAMMALS; NONIONIC SURFACTANTS;

CONCENTRATION (PROCESS); DNA; GENETIC ENGINEERING; HYDROPHOBICITY; NITROGEN COMPOUNDS; PH; SURFACE ACTIVE AGENTS; TEMPERATURE CONTROL; VISCOSITY;

AGGLOMERATION;

DNA; MACROGOL STEARATE; PLASMID DNA; POLYETHYLENEIMINE; SURFACTANT;

ANIMAL CELL; ANIMAL EXPERIMENT; AQUEOUS SOLUTION; ARTICLE; CELL CULTURE; CELL STRAIN COS7; CONCENTRATION (PARAMETERS); DILUTION; ELECTRICITY; GENE TARGETING; GENETIC TRANSFECTION; IONIC STRENGTH; MAMMAL CELL; MONKEY; NONHUMAN; PARTICLE SIZE; PH; PROTEIN AGGREGATION; PROTEIN STABILITY; TEMPERATURE; TOXICITY; VISCOSITY; ZETA POTENTIAL;

ANIMALS; CERCOPITHECUS AETHIOPS; COS CELLS; DIFFUSION; DNA; DRUG CARRIERS; DRUG DELIVERY SYSTEMS; GENE THERAPY; GENE TRANSFER TECHNIQUES; HYDROPHOBICITY; MACROMOLECULAR SUBSTANCES; NANOTUBES; PARTICLE SIZE; POLYETHYLENEIMINE; TRANSFECTION;

MAMMALIA;

EID: 19544362720     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.20444     Document Type: Article

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