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Volumn 275, Issue 5305, 1997, Pages 1478-1481

Block in anaphase chromosome separation caused by a telomerase template mutation

Author keywords

[No Author keywords available]

Indexed keywords

TELOMERASE;

EID: 0031047642     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.275.5305.1478     Document Type: Article
Times cited : (190)

References (34)
  • 1
    • 0003893137 scopus 로고    scopus 로고
    • Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
    • E. H. Blackburn and C. W. Greider, Eds., Telomeres (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1995).
    • (1995) Telomeres
    • Blackburn, E.H.1    Greider, C.W.2
  • 8
    • 0029655261 scopus 로고    scopus 로고
    • 4-specific oligonucleotide. Because the transformed cells also retain their endogenous chromosomal copy of the WT telomerase RNA gene, both WT and mutant repeats are synthesized onto the telomeres. A total of 115 telomeric repeats were sequenced from three different macronuclear telomere clones, 23% of which were mutated. The mutated repeats were confined almost exclusively to the most distal region of the telomere.
    • (1996) Mol. Cell. Biol. , vol.16 , pp. 66
    • Gilley, D.1    Blackburn, E.H.2
  • 9
    • 0026767479 scopus 로고
    • 4-specific oligonucleotide. Because the transformed cells also retain their endogenous chromosomal copy of the WT telomerase RNA gene, both WT and mutant repeats are synthesized onto the telomeres. A total of 115 telomeric repeats were sequenced from three different macronuclear telomere clones, 23% of which were mutated. The mutated repeats were confined almost exclusively to the most distal region of the telomere.
    • (1992) Proc. Natl. Acad. Sci. U.S.A. , vol.89 , pp. 9196
    • Gaertig, J.1    Gorovsky, M.A.2
  • 10
    • 0025279059 scopus 로고
    • 4-specific oligonucleotide. Because the transformed cells also retain their endogenous chromosomal copy of the WT telomerase RNA gene, both WT and mutant repeats are synthesized onto the telomeres. A total of 115 telomeric repeats were sequenced from three different macronuclear telomere clones, 23% of which were mutated. The mutated repeats were confined almost exclusively to the most distal region of the telomere.
    • (1990) Nature , vol.344 , pp. 126
    • Yu, G.-L.1    Bradley, J.D.2    Attardi, L.D.3    Blackburn, E.H.4
  • 11
    • 0029167574 scopus 로고
    • 4-specific oligonucleotide. Because the transformed cells also retain their endogenous chromosomal copy of the WT telomerase RNA gene, both WT and mutant repeats are synthesized onto the telomeres. A total of 115 telomeric repeats were sequenced from three different macronuclear telomere clones, 23% of which were mutated. The mutated repeats were confined almost exclusively to the most distal region of the telomere.
    • (1995) J. Eukaryot. Microbiol. , vol.42 , pp. 32
    • Romero, D.P.1    Blackburn, E.H.2
  • 13
    • 1842355442 scopus 로고    scopus 로고
    • note
    • Micronuclear phenotypic quantitation was performed in over 700 cells from 14 independent transformant cell lines in two separate experiments from 5 to 7 days after transformation. On average, 55 random micronuclei per cell line per time point were assessed for anaphase by 4′,6′-diamidino-2-phenylindole (DAPI) stain. Cells with no visible micronuclei were excluded from the determination. Length was judged by eye to be roughly equivalent to that observed in WT (elongated) or at least 50% longer than that found in WT, with no apparent separation at the midzone (hyperelongated). The proportion of micronuclei in anaphase varied with respect to cell line and time point (standard deviations for elongated and hyperelongated micronuclei at 7 days were ±7% and ±16%, respectively).
  • 15
    • 1842350420 scopus 로고    scopus 로고
    • note
    • In the ter1-43AA mutant, the macronucleus was never observed to divide while the micronucleus was arrested in anaphase. Hence, the onset of macronuclear division may require completion of micronuclear division. Therefore, although mutant telomeres also impair macronuclear division (6), possibly independently of the micronuclear telomere phenotype, such impairment of macronuclear division may not become evident while the micronucleus is mitotically blocked.
  • 19
    • 0028240044 scopus 로고
    • C. Holm, Cell 77, 955 (1994).
    • (1994) Cell , vol.77 , pp. 955
    • Holm, C.1
  • 26
    • 0029586089 scopus 로고
    • L. Chong et al., Science 270, 1663 (1995).
    • (1995) Science , vol.270 , pp. 1663
    • Chong, L.1
  • 28
    • 0030013594 scopus 로고    scopus 로고
    • H. Funabiki et al., Nature 381, 438 (1996).
    • (1996) Nature , vol.381 , pp. 438
    • Funabiki, H.1
  • 30
    • 1842321593 scopus 로고    scopus 로고
    • note
    • Cells were also imaged under wet buffer conditions without drying and were found to be quantitatively and qualitatively identical to dried cells: Anaphase micronuclei lengths were on average 10.46 μm by 2.06 μm by 2.49 μm for nondried (n = 7) and 10.95 μm by 1.98 μm by 2.23 μm (n = 3) for dried; non-dried chromosomes were 0.41 ± 0.09 μm wide (n = 69) versus 0.40 ±0.07 μm wide for dried chromosomes (n = 35).
  • 32
    • 0025058091 scopus 로고
    • D.A. Agard, Y. Hiraoka, P. Shaw, J. W. Sedat, Methods Cell Biol. 30, 353 (1989); Y. Hiraoka, J. W. Sedat, D. A. Agard, Biophys. J. 57, 325 (1990).
    • (1990) Biophys. J. , vol.57 , pp. 325
    • Hiraoka, Y.1    Sedat, J.W.2    Agard, D.A.3
  • 34
    • 1842402126 scopus 로고    scopus 로고
    • note
    • We thank J. C. Prescott, A. Bhattacharyya, and H. W. Bass for manuscript review. Supported by NIH grant GM26259 (to E.H.B.), an American Cancer Society postdoctoral fellowship (to K.E.K.), and by an NSF predoctoral fellowship (to B.P.H.).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.