Effects of freezing, thawing, and storage of human liver samples on the microsomal contents and activities of cytochrome P450 enzymes

Drug Metabolism and Disposition

Volumn 25, Issue 2, 1997, Pages 168-174

  Yamazaki, Hiroshi ^b   Inoue, Kiyoshi ^b   Turvy, Connie G ^b   Guengerich, F Peter ^b   Shimada, Tsutomu ^a  

^a OSAKA PREFECTURAL INSTITUTE OF PUBLIC HEALTH   (Japan)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

BUFURALOL; CHLORZOXAZONE; COUMARIN; CYTOCHROME P450; ETHOXYRESORUFIN; LIVER ENZYME; MEPHENYTOIN; NIFEDIPINE; TOLBUTAMIDE;

ARTICLE; CATALYSIS; DRUG OXIDATION; ENZYME ACTIVITY; ENZYME ANALYSIS; FREEZING; HUMAN; HUMAN CELL; LIVER MICROSOME; PRIORITY JOURNAL; STORAGE; THAWING;

CRYOPRESERVATION; CYTOCHROME P-450 ENZYME SYSTEM; DNA; ENZYME STABILITY; HUMANS; LIVER; MICROSOMES, LIVER; TIME FACTORS;

EID: 0031034166     PISSN: 00909556     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (51)

1. F. J. Gonzalez: The molecular biology of cytochrome P450s. Pharmacol. Rev. 40, 243-288 (1989).
2. F. P. Guengerich: Reactions and significance of cytochrome P-450 enzymes. J. Biol. Chem. 266, 10019-10022 (1991).
3. F. P. Guengerich and T. Shimada: Oxidation of toxic and carcinogenic chemicals by human cytochrome P-450 enzymes. Chem. Res. Toxicol. 4, 391-407 (1991).
4. F. J. Gonzalez, T. Aoyama, and H. V. Gelboin: Activation of promutagens by human cDNA-expressed cytochrome P450s. In "Mutation and the Environment, Part B: Metabolism, Testing Methods, and Chromosomes" (M.L. Mendelshon and R.J. Albertini, eds.), pp. 77-86, Wiley Liss, New York, 1990.
5. D. R. Nelson, L. Koymans, T. Kamataki, J. J. Stegeman, R. Feyereisen, D. J. Waxman, M. R. Waterman, O. Gotoh, M. J. Coon, R. W. Estabrook, I. C. Gunsalus, and D. W. Nebert: P450 superfamily: update on new sequences, gene mapping, accession numbers and nomenclature. Pharmacogenetics 6, 1-42 (1996).
6. D. W. Neben, D. R. Nelson, M. Adesnik, M. J. Coon, R. W. Estabrook, F. J. Gonzalez, F. P. Guengerich, I. C. Gunsalus, E. F. Johnson, B. Kemper, W. Levin, I. R. Phillips, R. Sato, and M. Waterman: The P450 superfamily: update listing of all genes and recommended nomenclature for the chromosomal loci. DNA 8, 1-13 (1989).
7. D. E. Ryan and W. Levin: Purification and characterization of hepatic microsomal cytochrome P-450. Pharmacol. Ther. 45, 153-239 (1990).
8. S. A. Wrighton, B. J. Ring, and M. Vandenbranden: The use of in vitro metabolism techniques in the planning and interpretation of drug safety studies. Toxicol. Pathol. 23, 199-208 (1995).
9. F. P. Guengerich: Human cytochrome P450 enzymes. In "Cytochrome P450" (P.R. Oritiz de Montellano, ed.), pp. 473-535, Plenum Press New York, 1995.
10. F. P. Guengerich, W. R. Brian, M. A. Sari, and J. T. Ross: Expression of mammalian cytochrome P-450 enzymes using yeast-based vectors. Methods Enzymol. 206, 130-145 (1991).
11. 1 and comparison to CYP1A2 and CYP2A3. Carcinogenesis 12, 355-359 (1991).
12. C. Wolfel, B. Heinrich-Hirsch, T. Schulz-Schalge, A. Seidel, H. Frank, U. Ramp, F. Wachter, F. J. Wiebel, F. Gonzalez, H. Greim, and J. Doehmer: Genetically engineered V79 Chinese hamster cells for stable expression of human cytochrome P450IA2. Eur. J. Pharmacol. 228, 95-102 (1992).
13. J. Buters, K. R. Korzekwa, K. L. Kunze, Y. Omata, J. P. Hardwick, and F. J. Gonzalez: cDNA-directed expression of human cytochrome P450 CYP3A4 using baculovirus. Drug Memb. Dispos. 22, 688-692 (1994).
14. H. Yamazaki, Z. Guo, M. Persmark, M. Mimura, K. Inoue, F. P. Guengerich, and T. Shimada: Bufuralol hydroxylation by cytochrome P450 2D6 and 1A2 enzymes in human liver microsomes. Mol. Pharmacol. 46, 568-577 (1994).
15. H. Yamazaki, K. Inoue, M. Mimura, Y. Oda, F. P. Guengerich, and T. Shimada: 7-Ethoxycoumarin O-deethylation catalyzed by cytochromes P450 1A2 and 2E1 in human liver microsomes. Biochem. Pharmacol. 51, 313-319 (1996).
16. R. Kato and Y. Yamazoe: The importance of substrate concentration in determining cytochromes P450 therapeutically relevant in vivo. Pharmacogenetics 4, 359-362 (1994).
17. T. Yasumori, K. Nagata, S. K. Yang, L. S. Chen, N. Murayama, Y. Yamazoe, and R. Kato: Cytochrome P450 mediated metabolism of diazepam in human and rat: involvement of human CYP2C in N-demethylation in the substrate concentration-dependent manner. Pharmacogenetics 3, 291-301 (1993).
18. T. Shimada, H. Yamazaki, M. Mimura, Y. Inui, and F. P. Guengerich: Interindividual variations in human fiver cytochrome P-450 enzymes involved in the oxidation of drugs, carcinogens and toxic chemicals: Studies with liver microsomes of 30 Japanese and 30 Caucasians. J. Pharmacol. Exp. Ther. 270, 414-423 (1994).
19. P. Wang, P. S. Mason, and F. P. Guengerich: Purification of human liver cytochrome P-450 and comparison to the enzyme isolated from rat liver. Arch. Biochem. Biophys. 199, 206-219 (1980).
20. T. Shimada, M. Iwasaki, M. V. Martin, and F. P. Guengerich: Human liver microsomal cytochrome P-450 enzymes involved in the bioactivation of procarcinogens detected by umu gene response in Salmonella typhimurium TA1535/pSK1002. Cancer Res. 49, 3218-3228 (1989).
21. M. Mimura, T. Baba, H. Yamazaki, S. Ohmori, Y. Inui, F. J. Gonzalez, F. P. Guengerich, and T. Shimada: Characterization of cytochrome P450 2B6 in human liver microsomes. Drug Metab. Dispos. 21, 1048-1056 (1993).
22. R. Peter, R. Böcker, P. H. Beaune, M. Iwasaki, F. P. Guengerich, and C. S. Yang: Hydroxylation of chlorzoxazone as a specific probe for human liver cytochrome P-450 IIE1. Chem. Res. Toxicol. 3, 566-573 (1990).
23. P. P. Wang, P. Beaune, L. S. Kaminsky, G. A. Dannan, F. F. Kadlubar, D. Larrey, and F. P. Guengerich: Purification and characterization of six cytochrome P-450 isozymes from human liver microsomes. Biochemistry 22, 5375-5383 (1983).
24. F. P. Guengerich: Analysis and characterization of enzymes. In "Principles and Methods of Toxicology" (A. W. Hayes, ed.), pp. 1259-1313, Raven Press, New York, 1994.
25. P. Sandhu, Z. Guo, T. Baba, M. V. Martin, R. H. Tukey, and F. P. Guengerich: Expression of modified human cytochrome P450 1A2 in Escherichia coli. Stabilization, purification, spectral characterization, and catalytic activities of the enzymes. Arch. Biochem. Biophys. 309, 168-177 (1994).
26. C. H. Yun, T. Shimada, and F. P. Guengerich: Purification and characterization of human liver microsomal cytochrome P-450 2A6. Mol. Pharmacol. 40, 679-685 (1991).
27. F. P. Guengerich, M. V. Martin, P. H. Beaune, P. Kremers, T. Wolff, and D. J. Waxman: Characterization of rat and human liver microsomal cytochrome P-450 forms involved in nifedipine oxidation, a prototype for genetic polymorphism in oxidative drug metabolism. J. Biol. Chem. 261, 5051-5060 (1986).
28. T. Shimada, K. S. Misono, and F. P. Guengerich: Human liver microsomal cytochrome P-450 mephenytoin 4-hydroxylase, a prototype of genetic polymorphism in oxidative drug metabolism. Purification and characterization of two similar forms involved in the reaction. J. Biol. Chem. 261, 909-921 (1986).
29. F. P. Guengerich, D. H. Kim, and M. Iwasaki: Role of human cytochrome P-450 IIE1 in the oxidation of many low molecular weight cancer suspects. Chem. Res. Toxicol. 4, 168-179 (1991).
30. L. S. Kaminsky, M. J. Fasco, and F. P. Guengerich: Production and application of antibodies to rat liver cytochrome P-450. Methods Enzymol. 74, 262-272 (1981).
31. T. Shimada and S. Nakamura: Cytochrome P-450-mediated activation of procarcinogens and promutagens to DNA-damaging products by measuring expression of umu gene in Salmonella typhimurium TA1535/pSK1002. Biochem. Pharmacol. 36, 1979-1987 (1987).
32. 5 in the oxidation of testosterone and nifedipine by recombinant cytochrome P450 3A4 and by human liver microsomes. Arch. Biochem. Biophys. 325, 174-182 (1996).
33. H. Yamazaki, Y.-F. Ueng, T. Shimada, and F. P. Guengerich: Roles of divalent metal ions in oxidations catalyzed by recombinant cytochrome P450 3A4 and replacement of NADPH-cytochrome P450 reductase with other flavoproteins, ferredoxin, and oxygen surrogates. Biochemistry 34, 8380-8389 (1995).
34. R. J. Pohl and J. R. Fouts: A rapid method for assaying the metabolism of 7-ethoxyresorufin by microsomal subcellular fractions. Anal. Biochem. 107, 150-155 (1980).
35. H. Yamazaki, M. Mimura, C. Sugahara, and T. Shimada: Catalytic roles of rat and human cytochrome P450 2A enzymes in testosterone 7α- and coumarin 7-hydroxylations. Biochem. Pharmacol. 48, 1524-1527 (1994).
36. T. Shimada, J. P. Shea, and F. P. Guengerich: A convenient assay for mephenytoin 4-hydroxylase activity of human liver microsomal cytochrome P-450. Anal. Biochem. 147, 174-179 (1985).
37. R. G. Knodell, S. D. Hall, G. R. Wilkinson, and F. P. Guengerich: Hepatic metabolism of tolbutamide: Characterization of the form of cytochrome P-450 involved in methyl hydroxylation and relationship to in vivo disposition. J. Pharmacol. Exp. Ther. 241, 1112-1119 (1987).
38. T. Kronbach, D. Mathys, J. Gut, T. Catin, and U. A. Meyer: High-performance liquid chromatographic assays for bufuralol 1′-hydroxylase, debrisoquine 4-hydroxylase, and dextromethorphan O-demethylase in microsomes and purified cytochrome P-450 isozymes of human liver. Anal. Biochem. 162, 24-32 (1987).
39. T. Omura and R. Sato: The carbon monoxide-binding pigment of liver microsomes. I. Evidence for its hemoprotein nature. J. Biol. Chem. 239, 2370-2378 (1964).
40. 5 reductase from rabbit liver microsomes. J. Biochem. (Tokyo) 78, 1057-1073 (1975).
41. M. Noshiro and T. Omura: Immunochemical study on the electron pathway from NADH to cytochrome P-450 of liver microsomes. J. Biochem. (Tokyo) 83, 61-77 (1978).
42. C. H. Williams, Jr. and H. Kamin: Microsomal triphosphopyridine nucleotide-cytochrome c reductase of liver. J. Biol. Chem. 237, 587-595 (1962).
43. F. P. Guengerich, P. Wang, and N. K. Davidson: Estimation of isozymes of microsomal cytochrome P-450 in rats, rabbits, and humans using immunochemical staining coupled with sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Biochemistry 21, 1698-1706 (1982).
44. O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall: Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275 (1951).
45. S. L. Wang, J. D. Huang, M. D. Lai, and J. J. Tsai: Detection of CYP2C9 polymorphism based on the polymerase chain reaction in Chinese. Pharmacogenetics 5, 37-42 (1995).
46. J. A. Goldstein and S. M. F. de Morais: Biochemistry and molecular biology of the human CYP2C subfamily. Pharmacogenetics 4, 285-299 (1994).
47. G. Powis, I. Jardine, R. V. Dyke, R. Weinshilboum, D. Moore, T. Wilke, W. Rhodes, R. Nelson, L. Benson, and C. Szumlanski: Foreign compound metabolism studies with human liver obtained as surgical waste. Relation to donor characteristics and effects of tissue storage. Drug Metab. Dispos. 16, 582-589 (1995).
48. P. Skett, C. Tyson, A. Guillouzo, and P. Maier: Report on the international workshop on the use of human in vitro liver preparations to study drug metabolism in drug development. Biochem. Pharmacol. 50, 280-285 (1995).
49. H. Sadano and T. Omura: Turnover of two drug-inducible forms of microsomal cytochrome P-450 in rat liver. J. Biochem. (Tokyo) 93, 1375-1383 (1983).
50. T. Omura: Cytochrome P450 linked mixed function oxidase: turnover of microsomal components and effects of inducers on the turnover of phospholipids, proteins, and specific enzymes. In "Hepatic Cytochrome P-450 Monooxygenase System" (J. B. Schenkman and D. Kupfer, eds.), pp. 295-305, Pergamon Press, Oxford, 1982.
51. R. E. Pearce, C. J. McIntyre, A. Madan, U. Sanzgiri, A. J. Draper, P. L. Bullock, D. C. Cook, L. A. Burton, J. Latham, C. Nevins, and A. Parkinson: Effects of freezing, thawing, and storing human liver microsomes on cytochrome P450 activity. Arch. Biochem. Biophys. 331, 145-169 (1996).