A specific real-time quantitative PCR detection system for event MON810 in maize YieldGard® based on the 3′-transgene integration sequence

Transgenic Research

Volumn 12, Issue 2, 2003, Pages 179-189

  Hernández, Marta ^a   Pla, Maria ^a   Esteve, Teresa ^a   Prat, Salomé ^a   Puigdomènech, Pere ^a   Ferrando, Alejandro ^a  

^a INSTITUT DE BIOLOGIA MOLECULAR DE BARCELONA   (Spain)

Author keywords

GMO; MON810 event; Real time PCR; TAIL PCR; TaqMan

Indexed keywords

ANIMALS; BASE SEQUENCE; CALIBRATION; DNA, PLANT; HUMANS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; REFERENCE STANDARDS; TRANSGENES; ZEA MAYS;

ANIMALIA; EMBRYOPHYTA; HEXAPODA; INSECTA; LEPIDOPTERA; OSTRINIA NUBILALIS; TRANSPOSONS; ZEA MAYS;

EID: 0038732762     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1022979624333     Document Type: Article

References (28)

1. Arumuganathan K and Earle ED (1991) Nuclear DNA content of some important plant species. Plant Mol Biol Rep 9: 208-218.
2. Berdal KG and Holst-Jensen A (2001) Roundup Ready® soybean event-specific real-time quantitative PCR assay and estimation of the practical detection and quantification limits in GMO analysis. Eur Food Res Technol 213: 432-438.
3. Dellaporta SL, Wood J and Hicks JB (1983) A plant DNA minipreparation version II. Plant Mol Biol Rep 1: 19-21.
4. European Commission (2000a) Commission Regulation (EC) No. 49/2000 of the Commission amending Council Regulation (EC) 1139/98 of January 10, 2000, concerning the compulsory indication on the labeling of certain foodstuffs produced from genetically modified organisms of particulars other than those provided for in Directive 79/112/EEC. Off J Eur Communities L6: 13-14.
5. European Commission (2000b) Commission Regulation (EC) No. 50/2000 of the Commission of January 10, 2000, concerning the labeling of foodstuffs and food ingredients containing additives and flavourings that have been genetically modified or have been produced from genetically modified organisms. Off J Eur Communities L6: 15-17.
6. European Parliament (2001) Council Directive (EC) No. 18/2001 of the European Parliament and of the Council of 12 March 2001 on the deliberate release into the environment of genetically modified organisms and repealing Council Directive 90/220/EC. Off J Eur Communities L106: 1-39.
7. Gilliliand G, Perrin S, Blanchard K and Bunn HF (1990) Analysis of cytokine mRNA and DNA: detection and quantitation by competitive polymerase chain reaction. Proc Natl Acad Sci USA 87: 2725-2729.
8. Held CA, Stevens J, Livak KI and Williams PM (1996) Real time quantitative PCR. Genome Res 6: 986-994.
9. Higuchi R, Dollinger G, Walsh PS and Griffith R (1992) Simultaneous amplification and detection of specific DNA sequences. Biotechnology 10: 413-417.
10. Holck A, Vaïtilingom M, Didierjean L and Rudi K (2002) 5′-nuclease PCR for quantitative event specific detection of the genetically modified Mon810 MaisGard™ maize. Eur Food Res Technol, in press.
11. James C (2001) Global Review of Commercialized Transgenic Crops. ISAAA Briefs No. 24.
12. Kay S and Van den Eede G (2001) The limits of GMO detection. Nat Biotechnol 19: 405.
13. Kleter GA, van der Krieken WM, Kok EJ, Bosch D, Jordi W and Gilissen LJWJ (2001) Regulation and exploitation of genetically modified crops. Nat Biotechnol 19: 1105-1110.
14. Kohli A, Leech M, Vain P, Laurie DA and Christou P (1998) Transgene organization in rice engineered through direct DNA transfer supports a two-phase integration mechanism mediated by the establishment of integration hot spots. Proc Natl Acad Sci USA 95: 7203-7208.
15. Liu Y-G, Mitsukawa N, Oosumi T and Whittier RF (1995) Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR. Plant J 8: 457-463.
16. Meyer R and Jaccaud E (1997) Detection of genetically modified soya in processed food products: development and validation of a PCR assay for the specific detection of glyphosate tolerant soybeans. Proceedings of the Ninth European Conference on Food Chemistry (EURO FOOD CHEM IX), FECS 1: 23-28.
17. Morrison TM, Weiss JJ and Wittwer CT (1998) Quantification of low-copy transcripts by continuous SYBR green I monitoring during amplification. Biotechniques 24: 954-962.
18. Pawlowski WP and Somers DA (1998) Transgenic DNA integrated into the oat genome is frequently interspersed by host DNA. Proc Natl Acad Sci USA 95: 12106-12110.
19. Register III JC, Peterson DJ, Bell PJ, Bullock PW, Evans IJ and Frame B et al. (1994) Structure and function of selectable and non-selectable transgenes in maize after introduction by particle bombardment. Plant Mol Biol 25: 951-961.
20. Studer E, Rhyner C, Lüthy J and Hübner P (1998) Quantitative competitive PCR for the detection of genetically modified soybean and maize. Z Lebensm Unters For A 207: 207-213.
21. Taverniers I, Windels P, Van Bockstaele E and De Loose M (2001) Use of cloned DNA fragments for event specific quantification of genetically modified organisms in pure and mixed food products. Eur Food Res Technol, in press.
22. Terry CF and Harris N (2001) Event specific detection of Roundup Ready™ Soya using two different real time PCR detection chemistries. Eur Food Res Technol, in press.
23. Tyagi S and Kramer FR (1996) Molecular Beacons: probes that fluoresce upon hybridization. Nat Biotechnol 14: 303-308.
24. Vaïtilingom M, Pijnenburg H, Gendre F and Brignon P (1999) Real-time quantitative PCR detection of genetically modified Maximizer maize and Roundup Ready soybean in some representative foods. J Agric Food Chem 47: 5261-5266.
25. Van Rie J, Jansens S, Höfte H, Degheele D and Van Mellaert H (1989) Specificity of Bacillus thuringiensis delta-endotoxins. Importance of specific receptors on the brush border membrane of the mid-gut of target insects. Eur J Biochem 186: 239-247.
26. Whitcombe D, Theaker J, Guy SP, Brown T and Little S (1999) Detection of PCR products using self-probing amplicons and fluorescence. Nat Biotechnol 17: 804-807.
27. Wittwer CT, Herrmann MG, Moss AA and Rasmussen RP (1997) Continuous fluorescence monitoring of rapid cycle DNA amplification. Biotechniques 22: 130-138.
28. Zimmermann A, Lüthy J and Pauli U (2000) Event specific transgene detection in Btl1 corn by quantitative PCR at the integration site. Lebensm Wiss Technol 33: 210-216.