Identification and characterization of T-DNA inserts by T-DNA fingerprinting

Euphytica

Volumn 123, Issue 1, 2002, Pages 75-84

  Theuns, I ^a   Windels, P ^a   De Buck, S ^b   Depicker, A ^b   Van Bockstaele, E ^a,c   De Loose, M ^a  

^a Centrum voor Landbouwkundig Onderzoek Gent CLO / Centre de Recherches Agronomiques Gand   (Belgium)

^b DEPARTMENT OF PLANT SYSTEMS BIOLOGY   (Belgium)

^c GHENT UNIVERSITY   (Belgium)

Author keywords

AFLP; Anchored PCR; GMO detection; T DNA integration

Indexed keywords

ARABIDOPSIS; EMBRYOPHYTA;

EID: 0036120031     PISSN: 00142336     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1014415619527     Document Type: Article

References (28)

1. The DNA sequences of T-DNA junctions suggest that complex T-DNA loci are formed by a recombination process resembling T-DNA integration
2. Vector backbone sequences are frequently integrated into the genome of transgenic Arabidopsis and tobacco plants
3. Determination of the T-DNA transfer and the T-DNA integration frequencies upon cocultivation of Arabidopsis thaliana root explants
4. T-DNA integration patterns in co-transformed plant cells suggest that T-DNA repeats originate from ligation of separate T-DNAs
5. Screening for transgenic lines with stable and suitable accumulation levels of a heterologous protein
6. Gene silencing results in instability of antibody production in transgenic plants
7. Post-transcriptional gene silencing in plants
8. A general method for gene isolation in tagging approaches: Amplification of insertion mutagenised sites (AIMS)
9. The introduction and expression of transgenes in plants
10. Illegitimate recombination in plants: A model for T-DNA integration
11. Agrobacterium-mediated plant transformation: A scientifically intriguing story with significant applications
12. Transgene copy number can be positively or negatively associated with transgene expression
13. T-DNA is organized predominantly in inverted repeat structures in plants transformed with Agrobacterium tumefaciens C58 derivatives
14. Integration of T-DNA binary vector 'backbone' sequences into the tobacco genome: Evidence for multiple complex patterns of integration
15. Direct repeats of T-DNA integrated in tobacco chromosome: Characterization of junction regions
16. T-DNA integration: A model of illigitimate recombination in plants
17. Role of inverted DNA repeats in transcriptional and post-transcriptional gene silencing
18. DNA rearrangements associated with the integration of T-DNA in tobacco: An example of multiple duplications of DNA around the integration target
19. L-DNA
20. Screening of transgenic plants by amplification of unknown genomic DNA flanking T-DNA
21. Post-transcriptional silencing of chalcone synthase in Petunia by inverted transgene repeats
22. Deviating T-DNA transfer from Agrobacterium tumefaciens to plants
23. Transposon Display identifies individual transposable elements in high copy number lines
24. Direct amplification from microsatellites: Detection of simple sequence repeat-based polymorphisms without cloning
25. AFLP: A new technique for DNA fingerprinting
26. Frequent collinear long transfer of DNA inclusive of the whole binary vector during Agrobacterium-mediate transformation
27. Identification, genetic localization, and allelic diversity of selectively amplified microsatellite polymorphic loci in lettuce and wild relatives (Lactuca spp.)
28. Display and isolation of transposon-flanking sequences starting from genomic DNA or RNA