Refolding and characterization of recombinant human GST-PD-1 fusion protein expressed in Escherichia coli

Acta Biochimica et Biophysica Sinica

Volumn 36, Issue 2, 2004, Pages 141-146

  Li, Da Wei ^a   Yu, Jian Feng ^a   Chen, Yong Jing ^a   Ma, Hong Bing ^a   Wang, Zheng Fei ^a   Zhu, Yi Bei ^a   Zhang, Xue Guang ^a  

^a SOOCHOW UNIVERSITY   (China)

Author keywords

Costimulatory molecule; GST PD 1; Refolding

Indexed keywords

COMPLEMENTARY DNA; GAMMA INTERFERON; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; INTERLEUKIN 2; MEMBRANE PROTEIN; PHYTOHEMAGGLUTININ; PROGRAMMED DEATH 1 PROTEIN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; CELL INCLUSION; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; GENE AMPLIFICATION; MOLECULAR CLONING; MOUSE; NONHUMAN; PLASMID; PROTEIN ANALYSIS; PROTEIN DENATURATION; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; SOLUBILITY; T LYMPHOCYTE ACTIVATION;

ANIMALIA; ESCHERICHIA COLI;

EID: 16544373396     PISSN: 05829879     EISSN: None     Source Type: Journal    
DOI: 10.1093/abbs/36.2.141     Document Type: Article

References (24)

1. Coyle AJ, Gutierrez-Ramos JC. The expanding B7 superfamily: Increasing complexity in costimulatory signals regulating T cell function. Nat Immunol, 2001, 2(3): 203-209
2. Sharpe AH, Freeman GJ. The B7-CD28 superfamily. Nat Rev Immunol, 2002, 2: 116-126
3. Carreno BM, Collins M. The B7 family of ligands and its receptors: New pathways for costimulation and inhibition of immune responses. Annu Rev Immunol, 2002, 20: 29-53
4. Ishida Y, Agata Y, Shibahara K, Honjo T. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J, 1992, 11: 3887-3895
5. Shinohara T, Taniwaki M£f¬Ishida Y, Kawaichi M, Honjo T. Structure and chromosomal localization of the human PD-1 gene (PDCD1). Genomics, 1994, 23: 704-706
6. Finger LR, Pu JY, Wasserman R, Vibhakar R, Louie E, Hardy RR, Burrows PD et al. The human PD-1 gene: Complete cDNA, genomic organization, and developmentally regulated expression in B cell progenitors. Gene, 1997, 197: 177-187
7. Dong HD, Zhu GF, Tamada K, Chen LP. B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion. Nat Med, 1999, 5: 1365-1369
8. Freeman GJ, Long AJ, Iwai Y, Bourque K, Chernova T, Nishimura H, Fitz LJ et al. Engagement of the PD-1 immunoinhibitory receptor by a novel B7 family member leads to negative regulation of lymphocyte activation. J Exp Med, 2000, 192: 1027-1034
9. Latchman Y, Wood CR, Chernova T, Chaudhary D, Borde M, Chernova I, Iwai Y et al. PD-L2 is a second ligand for PD-1 and inhibits T cell activation. Nat Immunol, 2001, 2: 261-268
10. Tseng SY, Otsuji M, Gorski K, Huang X, Slansky JE, Pai SI, Shalabi A et al. B7-DC, a new dendritic cell molecule with potent costimulatory properties for T cells. J Exp Med, 2001, 193: 839-846
11. Agata Y, Kawasaki A, Nishimura H, Ishida Y, Tsubata T, Yagita H, Honjo T. Expression of the PD-1 antigen on the surface of stimulated mouse T and B lymphocytes. Int Immunol, 1996, 8: 765-772
12. Nishimura H, Nose M, Hiai H, Minato N, Honjo T. Development of lupus-like autoimmune diseases by disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor. Immunity, 1999, 11: 141-151
13. Nishimura H, Okazaki T, Tanaka Y, Nakatani K, Hara M, Matsumori A, Sasayama S et al. Autoimmune dilated cardiomyopathy in PD-1 receptor-deficient mice. Science, 2001, 291: 319-322
14. Nishimura H, Honjo T. PD-1: An inhibitory immunoreceptor involved in peripheral tolerance. Trends Immunol, 2001, 22: 265-268
15. Ansari MJI, Salama AD, Chitnis T, Smith RN, Yagita H, Akiba H, Yamazaki T et al. The programmed death-1 (PD-1) pathway regulates autoimmune diabetes in nonobese diabetic (NOD) mice. J Exp Med, 2003, 198(1): 63-69
16. Salama AD, Chitnis T, Imitola J, Ansari MJI, Akiba H, Tushima F, Azuma M et al. Critical role of the programmed death-1 (PD-1) pathway in regulation of experimental autoimmune encephalomyelitis. J Exp Med, 2003, 198 (1): 71-78
17. Blazar BR, Carreno BM, Mortari AP, Carter L, Iwai Y, Yagita H, Nishimura H et al. Blockade of programmed death-1 engagement accelerates graft-versus-host disease lethality by an IFN-γ-dependent mechanism. J Immunol, 2003, 171: 1272-1277
18. Iwai Y, Ishida M, Tanaka Y, Okazaki T, Honjo T, Minato N. Involvement of PD-L1 on tumor cells in the escape from host immune system and tumor immunotherapy by PD-L1 blockade. PNAS//3/4ßÌå//, 2002, 99: 12293-12297
19. Iwai Y, Terawaki S, Ikegawa M, Okazaki T, Honjo T. PD-1 inhibits antiviral immunity at the effector phase in the liver. J Exp Med, 2003, 198(1): 39-50
20. Volkel D, Blankenfeldt W, Schomburg D. Large-scale production, purification and refolding of the full-length cellular prion protein from Syrian golden hamster in Escherichia coli using the glutathione S-transferase-fusion system. Eur J Biochem, 1998, 251(1-2): 462-71
21. Vibhakar R, Juan G, Traganos F, Darzynkiewicz Z, Finger LR. Activation-induced expression of human programmed death-1 gene in T-lymphocytes. Exp Cell Res, 1997, 232: 25-28
22. Mazanet MM, Hughes CC. B7-H1 is expressed by human endothelial cells and suppresses T cell cytokine synthesis. J Immunol, 2002, 169: 3581-3588
23. Carter L, Fouser LA, Jussif J, Fitz L, Deng B, Wood CR, Collins M et al. PD-1: PD-L inhibitory pathway affects both CD4(+) and CD8(+) T cells and is overcome by IL-2. Eur J Immunol, 2002, 32(3): 634-643
24. Cox GN, Pratt D, Smith D, McDermott MJ, van der Slice RW. Refolding and characterization of recombinant human soluble CTLA-4 expressed in Escherichia coli. Protein Expr Purif, 1999, 17(1): 26-32