Refolding and characterization of recombinant human soluble CTLA-4 expressed in Escherichia coli

Protein Expression and Purification

Volumn 17, Issue 1, 1999, Pages 26-32

  Cox, George N ^a   Pratt, Dickson ^a   Smith, Darin ^a   McDermott, Martin J ^a   Vanderslice, Rebecca W ^a  

^a AMGEN INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

B LYMPHOCYTE; BIOASSAY; DENDRITIC CELL; DIMERIZATION; DISULFIDE BOND; ESCHERICHIA COLI; HYBRID PROTEIN; IMMUNOGLOBULIN HEAVY CHAIN; LIGAND; LYMPHOCYTE ACTIVATION; MACROPHAGE; OXIDIZING AGENT; PROTEIN ANALYSIS; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; RECOMBINANT PROTEIN; SOLUBILITY; T LYMPHOCYTE;

ESCHERICHIA COLI;

EID: 0033213555     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1999.1093     Document Type: Article

References (24)

1. Lenschow D. J., Walunas T. L., Bluestone J. A. CD28/B7 system of T cell costimulation. Annu. Rev. Immunol. 14:1996;233-258.
2. Linsley P. S., Brady W., Urnes M., Grosmaire L. S., Damle N. K., Ledbetter J. A. CTLA-4 is a second receptor for the B cell activation antigen B7. J. Exp. Med. 174:1991;561-569.
3. Linsley P. S., Brady W., Grosmaire L., Aruffo A., Damle N. K., Ledbetter J. A. Binding of the B cell activation antigen B7 to CD28 costimulates T cell proliferation and interleukin 2 mRNA accumulation. J. Exp. Med. 173:1991;721-730.
4. Freedman A. S., Freeman G., Horowitz J. C., Daley J., Nadler L. M. B7, a B cell-restricted antigen that identifies preactivated B cells. J. Immunol. 139:1987;3260-3267.
5. Razi-Wolf Z., Freeman G. J., Galvin F., Benacerraf B., Nadler L., Resier H. Expression and function of the murine B7 antigen, the major costimulatory molecule expressed by peritoneal exudate cells. Proc. Natl. Acad. Sci. USA. 89:1992;4210-4214.
6. Thompson C. B., Lindsten T., Ledbetter J. A., Kunkel S. L., Young H. A., Emerson S. G., Leiden J. M., June C. H. CD28 activation pathway regulates the production of multiple T-cell-derived lymphokines/cytokines. Proc. Natl. Acad. Sci. USA. 86:1989;1333-1337.
7. Gimmi C. D., Freeman G. J., Gribben J. G., Sugita K., Freedman A. S., Morimoto C., Nadler M. B-cell surface antigen B7 provides a costimulatory signal that induces T cells to proliferate and secrete interleukin 2. Proc. Natl. Acad. Sci. USA. 88:1991;6575-6579.
8. Reiser H., Freeman G. J., Razi-Wolf Z., Gimmi C. D., Benacerraf B., Nadler L. M. Murine B7 antigen provides an efficient costimulatory signal for activation of murine T lymphocytes via the T cell receptor/ CD3 complex. Proc. Natl. Acad. Sci. USA. 89:1992;271-275.
9. Harding F. A., McArthur J. G., Gross J. A., Raulet D. H., Allison J. P. CD28-mediated signaling co-stimulates murine T cells and prevents induction of anergy in T cell clones. Nature. 356:1992;607-610.
10. Gimmi C. D., Freeman G. J., Gribben J. G., Gray G., Nadler L. M. Human T-cell clonal anergy is induced by antigen presentation in the absence of B7 co-stimulation. Proc. Natl. Acad. Sci. USA. 90:1993;6586-6590.
11. Tan P., Anasetti C., Hansen J. A., Melrose J., Brunvand M., Bradshaw J., Ledbetter J. A., Linsley P. S. Induction of alloantigen-specific hyporesponsiveness in human T lymphocytes by blocking interaction of CD28 with its natural ligand B7/BB1. J. Exp. Med. 177:1993;165-173.
12. Azuma M., Ito D., Yagita H., Okumura K., Phillips J. H., Lanier L. L., Somoza C. B70 antigen is a second ligand for CTLA-4 and CD28. Nature. 366:1993;76-79.
13. Freeman G. J., Gribben J. G., Boussiotis V. A., Ng J. W., Restivo V. A. Jr., Lombard L. A., Gray G. S., Nadler LJ. M. Cloning of B7-2: A CTLA-4 counter-receptor that costimulates human T cell proliferation. Science. 262:1993;909-911.
14. Engel P., Gribben J. G., Freeman G., Zhou L. J., Nozawa Y., Abe M., Nadler L. M., Wakawsa H., Tedder T. F. The B7-2(B70) co-stimulatory molecule of monocytes and activated B lymphocytes is the CD86 differentiation antigen. Blood. 84:1994;1402-1407.
15. Brunet J.-F., Denizot F., Luciani M.-F., Roux-Dosseto M., Suzan M., Mattei M.-G., Goldstein P. A new member of the immunoglobulin superfamily - CTLA-4. Nature. 328:1987;267-270.
16. Dariavach P., Mattei M.-G., Golstein P., Lefranc M.-P. Human Ig superfamily CTLA-4 gene: Chromosomal location and identity of protein sequence between murine and human CTLA-4 cytoplasmic domains. Eur. J. Immunol. 18:1988;1901-1905.
17. Aruffo A., Seed B. Molecular cloning of a CD28 cDNA by a high-efficiency COS cell expression system. Proc. Natl. Acad. Sci. USA. 84:1987;8573-8577.
18. Leahy D. J., Axel R., Hendrickson W. A. Crystal structure of a soluble form of the human T cell coreceptor CD8 at 2.6 A resolution. Cell. 68:1992;1145-1162.
19. Linsley P. S., Nadler S. G., Bajorath J., Peach R., Leung H. T., Rogers J., Bradshaw J., Stebbins M., Leytze G., Brady W., Malacko A. R., Marquardt H., Shaw S.-Y. Binding stoichiometry of the cytotoxic T lymphocyte-associated molecule-4 (CTLA-4). J. Biol. Chem. 270:1995;15417-15424.
20. Lindsten T., Lee K. P., Harris E. S., Petryniak B., Craighead N., Reynolds P. J., Lombard D. B., Freeman G. J., Nadler L. M., Gray G. S. Characterization of CTLA-4 structure and expression on human T cells. J. Immunol. 15:1993;3489-3499.
21. Eisenberg S. P, Evans R. J., Arend W. P., Verderber E., Brewer M. T., Hannum C. H., Thompson R. C. Primary structure and functional expression from complementary DNA of a human interleukin-1 receptor antagonist. Nature. 343:1989;341-346.
22. Laemmli U. K., Favre M. Maturation of the head of bacteriophage T4. I. DNA packaging events. J. Mol. Biol. 80:1973;575-599.
23. Cox G. N., McDrmott M. J., Merkel E., Stroh C. A., Ko S. C., Squires C. H., Gleason T. M., Russell D. Recombinant human insulin-like growth factor (IGF)- binding protein-1 inhibits somatic growth stimulated by IGF-I and growth hormone in hypophysectomized rats. Endocrinology. 135:1994;1913-1920.
24. June C. H., Bluestone J. A., Nadler L. M., Thompson C. B. The B7 and CD28 receptor families. Immunol. Today. 15:1994;321-331.