Site-directed mutagenesis of 2,4-dichlorophenoxyacetic acid/α- ketoglutarate dioxygenase. Identification of residues involved in metallocenter formation and substrate binding

Journal of Biological Chemistry

Volumn 275, Issue 17, 2000, Pages 12400-12409

  Hogani, Deborah A ^a,b   Smith, Sheila R ^a   Saari, Eric A ^a   McCracken, John ^a   Hausinger, Robert P ^a,b,c  

^a MICHIGAN STATE UNIVERSITY   (United States)

^b NONE   (United States)

^c Michigan State University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2,4 DICHLOROPHENOXYACETIC ACID; COPPER; HISTIDINE; MALTOSE BINDING PROTEIN; OXOGLUTARATE DIOXYGENASE; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; ENZYME ACTIVE SITE; ENZYME DEGRADATION; ENZYME ISOLATION; ENZYME SUBSTRATE COMPLEX; METAL BINDING; NONHUMAN; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; STRUCTURE ANALYSIS;

AMINO ACID SEQUENCE; BINDING SITES; CHROMATOGRAPHY, GEL; ELECTRON SPIN RESONANCE SPECTROSCOPY; HISTIDINE; KINETICS; LIGANDS; MIXED FUNCTION OXYGENASES; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PLASMIDS; PROTEIN BINDING; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

ESCHERICHIA COLI; FELIS CATUS; NEGIBACTERIA;

EID: 0034724670     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.275.17.12400     Document Type: Article

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