Thermotoga neapolitana bglB gene, upstream of lamA, encodes a highly thermostable β-glucosidase that is a laminaribiase

Microbiology

Volumn 143, Issue 11, 1997, Pages 3537-3542

  Zverlov, Vladimir V ^a   Volkov, Ilia Y ^a   Velikodvorskaya, Tatjana V ^a   Schwarz, Wolfgang H ^b  

^a INSTITUTE OF MOLECULAR GENETICS   (Russian Federation)

^b TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

BglB; Nucleotide sequence; Thermostability; Thermotoga neapolitana; glucosidase

Indexed keywords

BACTERIAL DNA; BETA GLUCOSIDASE; CELLOBIOSE; GLUCOSE; LAMINARAN; RECOMBINANT ENZYME;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENE; ENZYME ACTIVITY; GENETIC TRANSCRIPTION; GRAM NEGATIVE BACTERIUM; MICHAELIS CONSTANT; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PRIORITY JOURNAL; TEMPERATURE;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; THERMOTOGA NEAPOLITANA;

EID: 0030680965     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/00221287-143-11-3537     Document Type: Article

References (15)

1. Bause, E. & Legier, G. (1980). Isolation and structure of a tryptic glycopeptide from the active site of β-glucosidase A3 from Aspergillus wentii. Biochim Biophys Acta 626, 459-465.
2. Bok, J. D., Goers, S. K. & Eveleigh, D. E. (1994). Cellulase and xylanase systems of Thermotoga neapolitana. In Enzymatic Conversion of Biomass for Fuel Production, pp. 54-65. Edited by M. E. Himmel, J. O. Baker & R. P. Overend. ACS Symposium series 566. Washington, DC: American Chemical Society.
3. Dakhova, O. N., Kurepina, N. E., Zverlov, V. V., Svetlichnyi, V. A. & Velikodvorskaya, G. A. (1993). Cloning and expression in Escherichia coli of Thermotoga neapolitana genes coding for enzymes of carbohydrate substrate degradation. Biochem Biophys Res Commun 194, 1359-1364.
4. Gräbnitz, F., Rücknagel, K. P., Seiß, M. & Staudenbauer, W. L. (1989). Nucleotide sequence of the Clostridium thermocellum bglB gene encoding thermostable β-glucosidase B: homology to fungal β-glucosidases. Mol Gen Genet 217, 70-76.
5. Hanahan, D. (1983). Studies on transformation of Escherichia coli with plasmids. J Mol Biol 16, 557-580.
6. Henrissat, B. & Bairoch, A. (1993). New families in the classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem J 280, 309-316.
7. Huber, R. & Stetter, K. O. (1992). The order Thermotogales. In The Prokaryotes, 2nd edn, pp. 3809-3815. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K.-H. Schleifer. New York: Springer.
8. Liebl, W., Gabelsberger, J. & Schleifer, K.-H. (1994). Comparative amino acid sequence analysis of Thermotoga maritima βglucosidase (BglA) deduced from the nucleotide sequence of the gene indicates distant relationship between β-glucosidases of the BGA family and other families of β1,4-glycosyl hydrolases. Mol Gen Genet 242, 111-115.
9. Podbielski, A., Pohl, B., Woischnik, M., Körner, C., Schmidt, K.-H., Rozdzinski, E. & Leonard, B. A. B. (1996). Molecular characterization of group A streptococcal (GAS) oligopeptide permease (Opp) and its effect on cystein protease production. Mol Microbiol 21, 1087-1099.
10. Rojas, A. & Romeu, A. (1996). A sequence analysis of the βglucosidase sub-family B. FEBS Lett 378, 93-97.
11. Ruttersmith, L. D. & Daniel, R. M. (1993). Thermostable βglucosidase and β-xylosidase from Thermotoga sp. Strain FJSS3-B.1. Biochim Biophys Acta 1156, 167-172.
12. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
13. Wood, T. M. & Bhat, K. M. (1988). Methods for measuring cellulase activities. Methods Enzymol 160, 87-112.
14. Zverlov, V., Piotukh, K., Dakhova, O., Velikodvorskaya, G. & Borriss, R. (1996). The multidomain xylanase A of the hyperthermophilic bacterium Thertnotoga neapolitana is extremely thermoresistant. Appl Microbiol Biotechnol 45, 245-247.
15. Zverlov, V. V., Volkov, I. Y., Velikodvorskaya, T. V. & Schwarz, W. H. (1997). Highly thermostable endo-l,3-β-glucanase (laminarinase) Lam A from Thermotoga neapolitana: nucleotide sequence of the gene and characterization of the recombinant gene product. Microbiology 143, 1701-1708.