Purification, characterization, and molecular analysis of thermostable cellulases CelA and CelB from Thermotoga neapolitana

Applied and Environmental Microbiology

Volumn 64, Issue 12, 1998, Pages 4774-4781

  Bok, Jin Duck ^a,b   Yernool, Dinesh A ^a   Eveleigh, Douglas E ^a  

^a The State University of New Jersey   (United States)

^b Seoul National University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

ANALYSIS; BACTERIA; CELLULOSE; CELLULOSE DERIVATIVES; CHARACTERIZATION; ESCHERICHIA COLI; GENES; GLUCOSE; HYDROLYSIS; PH; PURIFICATION; THERMODYNAMIC STABILITY;

CARBOXYMETHYL CELLULOSE; CELLOBIOSE; MOLECULAR ANALYSIS; THERMOSTABLE CELLULASES; THERMOTOGA NEAPOLITANA; TRANSGLYCOSYLATION ACTIVITY;

ENZYMES;

CARBOXYMETHYLCELLULOSE; CELLOBIOSE; CELLULASE; GLUCOSE;

ARTICLE; CISTRON; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME GLYCOSYLATION; ENZYME PURIFICATION; GENE INDUCTION; GENE LIBRARY; GENE REPRESSION; HALF LIFE TIME; HYDROLYSIS; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; THERMOPHILIC BACTERIUM; THERMOSTABILITY; THERMOTOGA NEAPOLITANA;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; THERMOTOGA NEAPOLITANA;

EID: 0032403607     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.64.12.4774-4781.1998     Document Type: Article

References (54)

1. Adams, M. W. W. 1993. Enzymes and proteins from organisms that grow near and above 100°C. Annu. Rev. Microbiol. 47:627-658.
2. Altschul, S. F., W. Gish, W. Miller, E. W. Myers, and D. J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
3. Barr, B. K., Y.-L. Hsseh, B. Ganem, and D. B. Wilson. 1996. Identification of two functionally different classes of exocellulases. Biochemistry 35:586-592.
4. Béguin, P., and J.-P. Aubert. 1994. The biological degradation of cellulose. FEMS Microbiol. Rev. 13:25-58.
5. 3H-labelled, reduced and chromogenic cello-oligosaccharides. Biochem. J. 266:371-378.
6. Bisaria, V. S., and S. Mishra. 1989. Regulatory aspects of cellulase biosynthesis and secretion. Crit. Rev. Biotechnol. 9:61-103.
7. Bronnenmeier, K., A. Kern, W. Liebl, and W. L. Staudenbauer. 1995. Purification of Thermotoga maritima enzymes for the degradation of cellulosic materials. Appl. Environ. Microbiol. 61:1399-1407.
8. Claeyssens, M., H. Van Tilbeurgh, J. P. Kamerling, J. Berg, M. Varanska, and P. Biely. 1990. Studies of the cellulolytic system of the filamentous fungus Trichoderma reesei QM 9414. Biochem. J. 270:251-256.
9. Clarke, A. J. 1997. Biodegradation of cellulose: enzymology and biotechnology. Technomic Publishing Co. Inc., Lancaster, Pa.
10. Coutinho, J. B., N. R. Gilkes, R. A. J. Warren, D. G. Kilburn, and R. C. Miller, Jr. 1992. The binding of Cellulomonas fimi endoglucanase C (CenC) to cellulose and Sephadex is mediated by the N-terminal repeats. Mol. Microbiol. 6:1243-1252.
11. Devereux, J., P. Haeberli, and O. Smithies. 1988. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12:387-395.
12. Duffard, G. Y., C. M. McCutchen, P. Leduc, K. N. Parker, and R. M. Kelly. 1997. Purification and characterization of extremely thermostable β-mannanase, β-mannosidase, and α-galactosidase from Thermotoga neapolitana 5068. Appl. Environ. Microbiol. 63:169-177.
13. Gabelsberger, J., W. Liebl, and K.-H. Schleifer. 1993 Cloning and characterization of β-galactoside and β-glucoside hydrolyzing enzyme from T. maritima. FEMS Microbiol. Lett. 109:131-138.
14. Gebler, J., N. R. Gilkes, M. Claeyssens, D. B. Wilson, P. Béguin, W. W. Wakarchuk, D. G. Kilburn, R. C. Miller, R. A. J. Warren, and S. G. Withers. 1992. Stereoselective hydrolysis catalyzed by related β-1,4-glucanases and β 1,4-xylanases. J. Biol. Chem. 267:12559-12561.
15. Gold, L., and G. Stromo. 1987. Translational initiation, p. 1302-1307. In F. C. Niedhardt, J. L. Ingraham, K. B. Low, B. Magasanik, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella typhimurium: cellular and molecular biology. American Society for Microbiology, Washington, D.C.
16. Goyal, A. K., and D. E. Eveleigh. 1996. Cloning, sequencing and analysis of the ggh-A gene encoding a 1,4-β-D-glucan glucohydrolase from Microbispora hispora. Gene 172:93-98.
17. Gusakov, A. V., O. V. Protas, V. M. Chernoglazov, A. P. Sinitsyn, G. V. Kovalysheva, O. V. Shpanchenko, and O. V. Ermolova. 1991. Transglycosylation activity of cellobiohydrolase I from Trichoderma longibrachiatum on synthetic and natural substrates. Biochim. Biophys. Acta 1073:481-485.
18. Gusakov, A. V., A. P. Sinitsyn, G. H. Goldsteins, and A. A. Klyosov. 1984. Kinetics and mathematical model of hydrolysis and transglycosylation catalysed by cellobiase. Enzyme Microbiol. Technol. 6:275-282.
19. Halldorsdottir, S., E. T. Thorolfsdottir, R. Spilliaert, S. Thorbjarnardottir, A. Palsdottir, G. O. Hreggvidsson, J. K. Kristjansson, and G. Eggertsson. 1997. Unpublished data.
20. Han, S. J., Y. J. Yoo, and H. S. Kang. 1995. Characterization of a bifunctional cellulase and its structural gene. J. Biol. Chem. 270:26012-26019.
21. Henrissat, B., and A. Bairoch. 1996. Updating the sequence-based classification of glycosyl hydrolases. Biochem. J. 316:695-696.
22. Huber, R., T. A. Langworthy, H. Konig, M. Thomn, C. R. Woese, U. B. Sleytr, and K. O. Stetter. 1986. Thermotoga maritima sp. nov. represents a new genus of extremely thermophilic eubacteria growing up to 90°C. Arch. Microbiol. 144:324-333.
23. Irwin, D. C., M. Speizo, L. P. Walker, and D. B. Wilson. 1993. Activity studies of eight purified cellulases: specificity, synergism and binding domain effects. Biotechnol. Bioeng. 42:1002-1013.
24. King, M. R., D. A. Yernool, D. E. Eveleigh, and B. M. Chassy. 1998. Thermostable α-galactosidase from Thermotoga neapolitana: cloning, sequencing and expression. FEMS Microbiol. Lett. 163:37-42.
25. Klyosov, A. A. 1990. Trends in biochemistry and enzymology of cellulose degradation. Biochemistry 29:10577-10585.
26. Kubicek, C. P., R. Messner, F. Gruber, R. L. Mach, and E. M. Kubicek-Pranz. 1993. The Trichoderma cellulase regulatory puzzle: from the interior life of a secretory fungus. Enzyme Microbiol. Technol. 15:90-99.
27. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
28. Liebl, W., P. Ruile, K. Bronnenmeier, K. Riedel, F. Lottspeich, and I. Greif. 1996. Analysis of a Thermotoga maritima DNA fragment encoding two similar thermostable cellulases, CelA and CelB, and characterization of the recombinant enzymes. Microbiology 142:2533-2542.
29. Lin, E., and D. B. Wilson. 1987. Regulation of β-1,4-endoglucanase synthesis in Thermomonospora fusca. Appl. Environ. Microbiol. 53:1352-1357.
30. Mach, R. L., B. Seiboth, A. Myasnikov, R. Gonzalez, J. Strauss, A. M. Harkki, and C. P. Kubicek. 1995. The bgl1 gene of Trichoderma reesei QM 9414 encodes an extracellular, cellulose-inducible beta-glucosidase involved in cellulase induction by sophorose. Mol. Microbiol. 16:687-697.
31. Magasanik, B., and F. C. Niedhardt. 1987. Regulation of carbon and nitrogen utilization, p. 1318-1325. In F. C. Niedhardt, J. L. Ingraham, K. B. Low, B. Magasanik, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella typhimurium: cellular and molecular biology. American Society for Microbiology, Washington, D.C.
32. Meinke, A., H. G. Damude, P. Tomme, E. Kwan, D. G. Kilburn, R. C. Miller, R. A. J. Warren, and N. R. Gilkes. 1995. Enhancement of the endo-β-1,4-glucanase activity of an exocellobiohydrolase by deletion of a surface loop. J. Biol. Chem. 270:4383-4386.
33. Moser, B., N. R. Gilkes, D. G. Kilburn, R. A. J. Warren, and R. C. Miller, Jr. 1989. Purification and characterization of endoglucanase C of Cellulomonas fimi, cloning of the gene, and analysis of in vivo transcripts of the gene. Appl. Environ. Microbiol. 55:2480-2487.
34. Oliver, D. B. 1987. Periplasm and protein secretion, p. 56-69. In F. C. Niedhardt, J. L. Ingraham, K. B. Low, B. Magasanik, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella typhimurium. cellular and molecular biology. American Society for Microbiology, Washington, D.C.
35. Rixon, J. E., L. M. Ferreira, A. J. Durrant, J. I. Laurie, G. P. Hazelwood, and H. J. Gilbert. 1992. Characterization of the gene celD and its encoded product 1,4-beta-D-glucan glucohydrolase D from Pseudomonas fluorescens subsp. cellulosa. Biochem. J. 285:947-955.
36. Ruttersmith, L. D., and R. M. Daniel. 1991. Thermostable cellobiohydrolase from the thermophilic eubacterium Thermotoga sp. strain FjSS3-B.1. Biochem. J. 277:887-890.
37. Saarilahti, H. T., B. Henrissat, and E. T. Palva. 1990. CelS: a novel endoglucanase identified from Eriwinia carotovora subsp. carotovora. Gene 90:9-14.
38. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Laboratory Press, Cold Spring Harbor, N.Y.
39. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
40. Seiboth, B., S. Hakola, R. L. Mach, P. L. Suominen, and C. P. Kubicek. 1997. Role of four major cellulases in triggering of cellulase gene expression by cellulose in Trichoderma reesei. J. Bacteriol. 179:5318-5320.
41. Sinnott, M. L. 1990. Catalytic mechanisms of enzymic glycosyl transfer. Chem. Rev. 90:1171-1202.
42. Stetter, K. O. 1998. Hyperthermophiles: isolation, classification, and properties, p. 1-24. In K. Horikoshi and W. D. Grant (ed.), Extremophiles: microbial life in extreme environments. Wiley-Liss Inc., New York, N.Y.
43. Sunna, A., M. Moracci, M. Rossi, and G. Antranikian. 1997. Glycosyl hydrolases from hyperthermophiles. Extremophiles 1:2-23.
44. Swiatek, G., J. D. Bok, and D. A. Yernool. 1996. Cloning and sequence analysis of novel β-glucan-glucohydrolase A gene from Thermotoga neapolitana, abstr. O-77, p. 367. In Abstracts of the 96th General Meeting of the American Society for Microbiology 1996. American Society for Microbiology, Washington, D.C.
45. Teather, R. M., and P. J. Wood. 1982. Use of Congo red-polysaccharide interaction in the enumeration and characterization of cellulolytic bacteria from bovine rumen. Appl. Environ. Microbiol. 43:777-780.
46. Teeri, T. T. 1997. Crystalline cellulose degradation: new insight into the function of cellobiohydrolases. Tibtech 15:160-167.
47. Tomme, P., E. Kwan, N. R. Gilkes, D. G. Kilburn, and R. A. J. Warren. 1996. Charaterization of CenC, an enzyme from Cellulomonas fimi with endo-and exoglucanase activities. J. Bacteriol. 178:4216-4223.
48. Tomme, P., R. A. J. Warren, and N. R. Gilkes. 1995. Cellulose hydrolysis by bacteria and fungi. Adv. Microb. Physiol. 37:1-81.
49. Vargas, M., and K. M. Noll. 1996. Catabolite repression in the hyperthermophilic bacterium Thermotoga neapolitana is independent of cAMP. Microbiology 142:139-144.
50. Vieille, C., D. S. Brudette, and J. G. Zeikus. 1996. Thermozyme. Annu. Rev. Biotechnol. 2:1-80.
51. Vrsanska, M., and P. Biely. 1992. The cellobiohydrolase I from Trichoderma reesei QM 9414: action on cello-oligosaccharides. Carbohydr. Res. 227:19-27.
52. Warren, R. A. J. 1996. Microbial hydrolysis of polysaccharides. Annu. Rev. Microbiol. 50:183-212.
53. Winterhalter, C., and W. Liebl. 1995. Two extremely thermostable xylanases of the hyperthermophilic becterium Thermotoga maritima MSB8. Appl. Environ. Microbiol. 61:1810-1815.
54. Wood, T. M., and K. M. Bhat. 1988. Methods for measuring cellulase actiities. Methods Enzymol. 160:87-112.