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Volumn 14, Issue 7, 2004, Pages 1625-1628

Evidences for the formation of bisbenzamidine-heme complexes in cell-free systems

Author keywords

Bisbenzamidine; Cell free systems; Chloroquine; Heme; Pentamidine

Indexed keywords

BENZAMIDINE; HEMATIN; HEME;

EID: 1542719142     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2004.01.070     Document Type: Article
Times cited : (11)

References (34)
  • 29
    • 1542535852 scopus 로고    scopus 로고
    • note
    • 9,10 A solution of hemin (15.2 mg) in aqueous sodium hydroxide 0.1 M (3 mL) was thermostatted at 60°C and diluted with warm solutions (60°C) of hydrochloric acid 1.0 M (0.3 mL) and buffer acetate 5.0 M (pH 5.0; 1.8 mL). Three equivalents (70 mmol) of the compound to be tested were added and the mixture was stirred at 60°C for 30 min. The reaction medium was cooled down in an ice bath for 5 min. The solid was filtered, washed with water and dried before analysis.
  • 30
    • 1542640706 scopus 로고    scopus 로고
    • note
    • 6 A stock solution (10 mL) of the studied compound (solution A) was prepared by dissolving 2 μmol of that compound in a mixture of DMSO (1 mL) and HEPES buffer 0.2 M (pH 7.0). A stock solution (10 mL) of hemin (solution B) was prepared by dissolving 2 μmol (1.3 mg) of hemin in a mixture of aqueous sodium hydroxide 0.1 M (5 mL) and HEPES buffer 0. 2 M. The solution (5 mL) of the studied compound that was scanned was obtained by mixing solution A (1 mL), solution B (1 mL), and HEPES buffer 0.2 M. The blank (5 mL) that was scanned was obtained in a similar way but in the absence of the compound and hemin. The solution (5 mL) of hemin that was scanned was also obtained in a similar way but in the absence of the compound. All solutions were prepared in volumetric flasks.
  • 31
    • 1542535854 scopus 로고    scopus 로고
    • note
    • 2·2HCl (439.34): C, 51.94; H, 5.51; N, 19.13. Found: C, 51.79; H, 5.31; N, 18.89. (M-H-W Laboratories, Phoenix, AZ).
  • 33
    • 1542535850 scopus 로고
    • Chem. Abstr. 1966, 64, 5102e
    • Wander A. Brit. 1:1965;7. Chem. Abstr. 1966, 64, 5102e.
    • (1965) Brit , vol.1 , pp. 7
    • Wander, A.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.