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1542535852
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note
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9,10 A solution of hemin (15.2 mg) in aqueous sodium hydroxide 0.1 M (3 mL) was thermostatted at 60°C and diluted with warm solutions (60°C) of hydrochloric acid 1.0 M (0.3 mL) and buffer acetate 5.0 M (pH 5.0; 1.8 mL). Three equivalents (70 mmol) of the compound to be tested were added and the mixture was stirred at 60°C for 30 min. The reaction medium was cooled down in an ice bath for 5 min. The solid was filtered, washed with water and dried before analysis.
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1542640706
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note
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6 A stock solution (10 mL) of the studied compound (solution A) was prepared by dissolving 2 μmol of that compound in a mixture of DMSO (1 mL) and HEPES buffer 0.2 M (pH 7.0). A stock solution (10 mL) of hemin (solution B) was prepared by dissolving 2 μmol (1.3 mg) of hemin in a mixture of aqueous sodium hydroxide 0.1 M (5 mL) and HEPES buffer 0. 2 M. The solution (5 mL) of the studied compound that was scanned was obtained by mixing solution A (1 mL), solution B (1 mL), and HEPES buffer 0.2 M. The blank (5 mL) that was scanned was obtained in a similar way but in the absence of the compound and hemin. The solution (5 mL) of hemin that was scanned was also obtained in a similar way but in the absence of the compound. All solutions were prepared in volumetric flasks.
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31
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1542535854
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note
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2·2HCl (439.34): C, 51.94; H, 5.51; N, 19.13. Found: C, 51.79; H, 5.31; N, 18.89. (M-H-W Laboratories, Phoenix, AZ).
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1542535850
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