Fluorescent tagging of rhythmically active respiratory neurons within the pre-bötzinser complex of rat medullary slice preparations

Journal of Neuroscience

Volumn 25, Issue 10, 2005, Pages 2591-2596

  Pagliardini, Silvia ^a   Adachi, Tadafumi ^a   Ren, Jun ^a   Funk, Gregory D ^a   Greer, John J ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

Author keywords

Apnea; Brainstem; Breathing; Medulla; Neurokinin receptors; Substance P

Indexed keywords

NEUROKININ 1 RECEPTOR; SUBSTANCE P; TETRAMETHYLRHODAMINE ETHYL ESTER;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; MEDULLA OBLONGATA; NONHUMAN; PACEMAKER; PATCH CLAMP; PRE BOTZINGER COMPLEX; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; RESPIRATION CENTER; RESPIRATION CONTROL; RESPIRATORY NERVE CELL; TARGET CELL; TISSUE SECTION; WHOLE CELL;

ANIMALS; FLUORESCENT DYES; ISOTOPE LABELING; MEDULLA OBLONGATA; MOLECULAR PROBE TECHNIQUES; MOLECULAR PROBES; NEURONS; PERIODICITY; RATS; RATS, SPRAGUE-DAWLEY; RECEPTORS, NEUROKININ-1; RESPIRATION; RESPIRATORY MECHANICS; SUBSTANCE P;

EID: 14944340229     PISSN: 02706474     EISSN: None     Source Type: Journal    
DOI: 10.1523/JNEUROSCI.4930-04.2005     Document Type: Article

References (30)

1. Bennett VJ, Simmons MA (2001) Analysis of fluorescently labeled substance P analogs: binding, imaging and receptor activation. BMC Chem Biol 1:1-12.
2. Butera RJ, Rinzel J, Smith JC (1999) Models of respiratory rhythm generation in the pre-Bötzinger complex. I. Bursting pacemaker neurons. J Neurophysiol 82:382-397.
3. Del Negro CA, Morgado-Valle C, Feldman JL (2002) Respiratory rhythm: an emergent network property? Neuron 34:821-830.
4. Frermann D, Keller BU, Richter DW (1999) Calcium oscillations in rhythmically active respiratory neurones in the brainstem of the mouse. J Physiol (Lond) 515:119-131.
5. Gray PA, Rekling JC, Bocchiaro CM, Feldman JL (1999) Modulation of respiratory frequency by peptidergic input to rhythmogenic neurons in the pre-Bötzinger complex. Science 286:1566-1568.
6. Gray PA, Janczewski WA, Mellen N, McCrimmon DR, Feldman JL (2001) Normal breathing requires pre-Bötzinger complex neurokinin-1 receptor-expressing neurons. Nat Neurosci 4:927-930.
7. Guyenet PG, Sevigny CP, Weston MC, Stornetta RL (2002) Neurokinin-1 receptor-expressing cells of the ventral respiratory group are functionally heterogeneous and predominantly glutamatergic. J Neurosci 22:3806-3816.
8. Harrison S, Geppetti P (2001) Substance P. Int J Biochem Cell Biol 33:555-576.
9. Horie M, Miyashita T, Watabe K, Takeda Y, Kawamura K, Kawano H (2000) Immunohistochemical localization of substance P receptors in the mid-line glia of the developing rat medulla oblongata with special reference to the formation of raphe nuclei. Brain Res Dev Brain Res 121:197-207.
10. Johnson SM, Smith JC, Funk GD, Feldman JL (1994) Pacemaker behavior of respiratory neurons in medullary slices from neonatal rat. J Neurophysiol 72:2598-2608.
11. Johnson SM, Smith JC, Feldman JL (1996) Modulation of respiratory rhythm in vitro: role of Gi/o protein-mediated mechanisms. J Appl Physiol 80:2120-2133.
12. Koshiya N, Smith JC (1999) Neuronal pacemaker for breathing visualized in vitro. Nature 400:360-363.
13. Liu Q, Wong-Riley MT (2002) Postnatal expression of neurotransmitters, receptors, and cytochrome oxidase in the rat pre-Botzinger complex. J Appl Physiol 92:923-934.
14. Mantyh PW (2002) Neurobiology of substance P and the NK1 receptor. J Clin Psychiatry 63 [Suppl 11]:6-10.
15. Manzke T, Guenther U, Ponimaskin EG, Haller M, Dutschmann M, Schwarzacher S, Richter DW (2003) 5-HT4(a) receptors avert opioid-induced breathing depression without loss of analgesia. Science 301:226-229.
16. Murakoshi T, Suzue T, Tamai S (1985) A pharmacological study on respiratory rhythm in the isolated brainstem-spinal cord preparation of the newborn rat. Br J Pharmacol 86:95-104.
17. Onimaru H, Homma I (2003) A novel functional neuron group for respiratory rhythm generation in the ventral medulla. J Neurosci 23:1478-1486.
18. Pagliardini S, Ren J, Greer JJ (2003) Ontogeny of the pre-Botzinger complex in perinatal rats. J Neurosci 23:9575-9584.
19. Pilowsky PM, Feldman JL (2001) Identifying neurons in the pre-Bötzinger complex that generate respiratory rhythm: visualizing the ghost in the machine. J Comp Neurol 434:125-127.
20. Rekling JC, Feldman JL (1998) Pre-Bötzinger complex and pacemaker neurons: hypothesized site and kernel for respiratory rhythm generation. Annu Rev Physiol 60:385-405.
21. Roosterman D, Cottrell GS, Schmidlin F, Steinhoff M, Bunnett NW (2004) Recycling and resensitization of the neurokinin 1 receptor. Influence of agonist concentration and Rab GTPases. J Biol Chem 279:30670-30679.
22. Smith JC, Liu G, Feldman JL (1988) Intracellular recording from phrenic motoneurons receiving respiratory drive in vitro. Neurosci Lett 88:27-32.
23. Smith JC, Ellenberger HH, Ballanyi K, Richter DW, Feldman JL (1991) Pre-Bötzinger complex: a brainstem region that may generate respiratory rhythm in mammals. Science 254:726-729.
24. Smith JC, Ballanyi K, Richter DW (1992) Whole-cell patch-clamp recordings from respiratory neurons in neonatal rat brainstem in vitro. Neurosci Lett 134:153-156.
25. Smith JC, Butera RJ, Koshiya N, Del Negro C, Wilson CG, Johnson SM (2000) Respiratory rhythm generation in neonatal and adult mammals: the hybrid pacemaker-network model. Respir Physiol 122:131-147.
26. Stornetta RL, Rosin DL, Wang H, Sevigny CP, Weston MC, Guyenet PG (2003) A group of glutamatergic interneurons expressing high levels of both neurokinin-1 receptors and somatostatin identifies the region of the pre-Bötzinger complex. J Comp Neurol 455:499-512.
27. Suzue T (1984) Respiratory rhythm generation in the in vitro brain stem-spinal cord preparation of the neonatal rat. J Physiol (Lond) 354:173-183.
28. Turcatti G, Zoffmann S, Lowe III JA, Drozda SE, Chassaing G, Schwartz TW, Chollet A (1997) Characterization of non-peptide antagonist and peptide agonist binding sites of the NK1 receptor with fluorescent ligands. J Biol Chem 272:21167-21175.
29. Wang X, Marvizon JC (2002) Time-course of the internalization and recycling of neurokinin 1 receptors in rat dorsal horn neurons. Brain Res 944:239-247.
30. Yamamoto Y, Onimaru H, Homma I (1992) Effect of substance P on respiratory rhythm and pre-inspiratory neurons in the ventrolateral structure of rostral medulla oblongata: an in vitro study. Brain Res 599:272-276.