Analysis of fluorescently labeled substance P analogs: Binding, imaging and receptor activation

BMC Chemical Biology

Volumn 1, Issue , 2001, Pages 1-12

  Bennett, Vicki J ^a   Simmons, Mark A ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM ION; FLUORESCENT DYE; POTASSIUM ION; SUBSTANCE P DERIVATIVE;

ANIMAL CELL; ARTICLE; CHO CELL; CONTROLLED STUDY; FLUORESCENCE; IMAGING SYSTEM; ISOTOPE LABELING; LIGAND BINDING; NERVE CELL; NONHUMAN; POTASSIUM CURRENT; PROTEIN ANALYSIS; PROTEIN BINDING; RECEPTOR BINDING;

ALEXA; ANIMALIA; CRICETINAE; CRICETULUS GRISEUS;

EID: 0011913322     PISSN: 14726769     EISSN: None     Source Type: Journal    
DOI: 10.1186/1472-6769-1-1     Document Type: Article

References (21)

1. Otsuka M, Yoshioka K: Neurotransmitter functions of mammalian tachykinins. Physiol Rev 1993, 73:229-308
2. Chang MM, Leeman SE: Isolation of a sialogogic peptide from bovine hypothalamic tissue and its characterization as substance P. J Biol Chem 1970, 245:4784-4790
3. Von Euler US, Gaddum JH: An unidentified depressor substance in certain tissue extracts. J Physiol 1931, 72:74-86
4. Adams PR, Brown DA: Substance P inhibits the M-current in bullfrog sympathetic neurones. Br J Pharmac 1983, 79:330-333
5. Perrine SA, Whitehead TL, Hicks RP, Szarek JL, Krause JE, Simmons MA: Solution structures in SDS micelles and functional activity at the bullfrog substance P receptor of ranatachykinin peptides. J Med Chem 2000, 43:1741-1753
6. Torrens Y, Beaujouan JC, Saffroy M, Glowinski J, Tence M: Functional coupling of the NK1 tachykinin receptor to phospholipase D in Chinese hamster ovary cells and astrocytoma cells. J Neurochem 1998, 70:2091-2098
7. Li HS, Leeman SE, Slack BE, Hauser G, Saltsman WS, Krause JE, Blusztajn JK, Boyd ND: A substance P (neurokinin-1) receptor mutant carboxyl-terminally truncated to resemble a naturally occurring receptor isoform displays enhanced responsiveness and resistance to desensitization. PNAS 1997, 94:9475-9480
8. Akasu T, Ishimatsu M, Yamada K: Tachykinins cause inward current through NK1 receptors in bullfrog sensory neurons. Brain Res 1996, 713:160-167
9. Simmons MA, Brodbeck RM, Karpitskiy VV, Schneider CR, Neff DPA, Krause JE: Molecular characterization and functional expression of a substance P receptor from the sympathetic ganglion of Rana catesbeiana. Neurosci 1997, 79:1219-1229
10. Heithier H, Hallmann D, Boege F, Reiländer H, Dees C, Jaeggi KA, Arndt-Jovin D, Jovin TM, Helmreich EJM: Synthesis and properties of fluorescent β-adrenoceptor ligands. Biochem 1994, 33:9126-9134
11. Tota MR, Daniel S, Sirotina A, Mazina DE, Fong TM, Longmore J, Strader CD: Characterization of a fluorescent substance P analog. Biochem 1994, 33:13079-13086
12. Turcatti G, Zoffmann S, Lowe J, Drozda S, Chassaing G, Schwartz T, Chollet A: Characterization of non-peptide antagonist and peptide agonist binding sites of the NK1 receptor with fluorescent ligands. J Biol Chem 1997, 272:21167-2117
13. Li YM, Marnerakis M, Stimson ER, Maggio JE: Mapping peptide-binding domains of the substance P (NK-1) receptor from P388D1 cells with photolabile agonists. J Biol Chem 1995, 270:1213-1220
14. Panchuk-Voloshina N, Haugland RP, Bishop-Stewart J, Bhalgat MK, Millard PJ, Mao F, Leung W, Haugland RP: Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photo-stable conjugates. J Histochem Cytochem 1999, 47:1179-1188
15. Arttamangkul S, Alvarez-Maubecin V, Thomas G, Williams JT, Grandy DK: Binding and internalization of fluorescent opioid peptide conjugates in living cells. Mol Pharm 2000, 58:1570-1580
16. Buku A, Masur S, Eggena P: Synthesis and characterization of fluorescein- and tetramethylrhodamine-labeled probes for vasotocin receptors. Am J Physiol 1989, 257:E804-808
17. Eggena P, Buku A: Synthesis and characterization of a long-acting fluorescent analogue of vasotocin. Biol Cell 1989, 66:1-6
18. Raddatz R, Crankshaw CL, Snider RM, Krause JE: Similar rates of phosphatidylinositol hydrolysis following activation of wild-type and truncated rat neurokinin-1 receptors. J Neurochem 1995, 64:1183-1191
19. Simmons MA, Mather RJ: Selectivity of the effects of guanosine-5′- O-(2-thiodiphosphate) on agonist inhibition of the M current in amphibian sympathetic neurons. J Neurosci 1991, 11:2130-2134
20. Simmons MA, Mather RJ: Intracellular guanosine-5′-O-(2- thiodiphosphate) alters the dynamics of receptor-mediated responses in bullfrog sympathetic neurons. Mol Pharm 1992, 41:527-534
21. Simmons MA, Schneider CR, Krause JE: Regulation of the responses to gonadotropin releasing hormone, muscarine, and substance P in sympathetic neurons by changes in cellular constituents and intracellular application of peptide fragments of the substance P receptor. J Pharm Exp Ther 1994, 271:581-589