Inhibition of Rho kinase modulates radiation induced fibrogenic phenotype in intestinal smooth muscle cells through alteration of the cytoskeleton and connective tissue growth factor expression

Gut

Volumn 54, Issue 3, 2005, Pages 336-343

  Bourgier, C ^b   Haydont, V ^b   Milliat, F ^b   Francois A ^b   Holler, V ^b   Lasser, P ^c   Bourhis, J ^b,c   Mathe D ^b   Vozenin Brotons, M C ^a,b  

^a Laboratoire UPRES EA 27 10   (France)

^b INSTITUT DE RADIOPROTECTION ET DE SÛRETÉ NUCLÉAIRE IRSN   (France)

^c INSTITUT GUSTAVE ROUSSY   (France)

Author keywords

[No Author keywords available]

Indexed keywords

4 (1 AMINOETHYL) N (4 PYRIDYL)CYCLOHEXANECARBOXAMIDE; ACTIN; COMPLEMENTARY DNA; CONNECTIVE TISSUE GROWTH FACTOR; RHO KINASE;

ACTIN FILAMENT; ADULT; AGED; ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CELL ISOLATION; CLINICAL ARTICLE; CONTROLLED STUDY; CYTOSKELETON; DNA MICROARRAY; ENZYME INHIBITION; EXTRACELLULAR MATRIX; FIBROSIS; GEL MOBILITY SHIFT ASSAY; HUMAN; HUMAN TISSUE; ILEUM; IN VITRO STUDY; INTESTINE BIOPSY; INTESTINE MUCOSA; INTESTINE MUSCLE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FAMILY; RADIATION ENTEROPATHY; REAL TIME POLYMERASE CHAIN REACTION; SMOOTH MUSCLE FIBER; WESTERN BLOTTING;

ACTINS; ADULT; AGED; AGED, 80 AND OVER; AMIDES; CELL DIFFERENTIATION; CELLS, CULTURED; CYTOSKELETON; DNA-BINDING PROTEINS; ENTERITIS; ENZYME INHIBITORS; FEMALE; FIBROSIS; GENE EXPRESSION PROFILING; HUMANS; ILEUM; IMMEDIATE-EARLY PROTEINS; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; MALE; MIDDLE AGED; MUSCLE, SMOOTH; NF-KAPPA B; PROTEIN-SERINE-THREONINE KINASES; PYRIDINES; RADIATION INJURIES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RHO GTP-BINDING PROTEINS; SIGNAL TRANSDUCTION;

EID: 14544278377     PISSN: 00175749     EISSN: None     Source Type: Journal    
DOI: 10.1136/gut.2004.051169     Document Type: Article

References (32)

1. Vozenin-Brotons MC, Milliat F, Sabourin JC, et al. Fibrogenic signals in patients with radiation enteritis are associated with increased connective tissue growth factor expression, Int J Radiat Oncol Biol Phys 2003;56:561-72.
2. Strup-Perrot C, Mathe D, Linard C, et al. Globol gene expression profiles reveal an increase in mRNA levels of collagens, MMPs and TIMPs in late radiation enteritis. Am J Physiol Gastrointest Liver Physiol 2004;287:G875-85.
3. Graham MF. Pathogenesis of intestinal strictures in Crohn's disease-An Update. Inflamm Bowel Dis 1995;1:220-7.
4. Macdonald TT. A mouse model of intestinal fibrosis? Gastroenterology 2003;125:1889-92.
5. Husebye E, Hauer-Jensen M, Kjorstod K, et al. Severe late radiation enteropathy is characterized by impaired motility of proximal small intestine. Dig Dis Sci 1994;39:2341-9.
6. Tomasek JJ, Gabbiani G, Hinz B, et al. Myofibroblasts and mechano-regulation of connective tissue remodelling. Nat Rev Mol Cell Biol 2002;3:349-63.
7. Brittingham J, Phiel C, Trzyna WC, et al. Identification of distinct molecular phenotypes in cultured gastrointestinal smooth muscle cells. Gastroenterology 1998;115:605-17.
8. Ridley AJ. Rho GTPases and cell migration. J Cell Sci 2001;114:2713-22.
9. Ridley AJ. Rho family proteins: coordinating cell responses. Trends Cell Biol 2001;11:471-7.
10. Riento K, Ridley AJ. Rocks: multifunctional kinases in cell behaviour. Nat Rev Mol Cell Biol 2003;4:446-56.
11. Heusinger-Ribeiro J, Eberlein M, Wahab NA, et al. Expression of connective tissue growth factor in human renal fibroblasts: regulatory roles of RhoA and cAMP. J Am Soc Nephrol 2001;12:1853-61.
12. Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biocnem 1987;162:156-9.
13. Vozenin-Brotons MC, Milliat F, Linard C, et al. Gene expression profile in human late radiation enteritis obtained by high-density cDNA array hybridization. Radiat Res 2004;161:299-311.
14. Schreiber E, Matthias P, Muller MM, et al. Rapid detection of octamer binding proteins with 'mini-extracts', prepared from a small number of cells. Nucleic Acids Res 1989;17:0419.
15. Chamley-Campbell J, Campbell GR, Ross R. The smooth muscle cell in culture. Physiol Rev 1979;59:1-61.
16. Graham M, Glück U, Shah M, et al. b-Tropomyosin and a-actin are phenotypic markers for human intestinal smooth muscle cells in vitro. Mol Cell Differ 1994;2:45-60.
17. Mahida YR, Beltinger J, Makh S, et al. Adult human colonic subepithelial myofibroblasts express extracellular matrix proteins and cyclooxygenase-1 and -2. Am J Physiol 1997;273:G1341-8.
18. Holmes A, Abraham DJ, Sa S, et al. CTGF and SMADs, maintenance of scleroderma phenotype is independent of SMAD signaling. J Biol Chem 2001;276:10594-601.
19. McKaig BC, Hughes K, Tighe PJ, et al. Differential expression of TGF-beta isoforms by normal and inflammatory bowel disease intestinal myofibroblasts. Am J Physiol Cell Physiol 2002;282:C172-82.
20. McKaig BC, McWilliams D, Watson SA, et al. Expression and regulation of tissue inhibitor of metalloproteinase-1 and matrix metalloproteinases by intestinal myofibroblasts in inflammatory bowel disease. Am J Pathol 2003;162:1355-00.
21. Breitenlechner C, Gassel M, Hidaka H, et al. Protein kinase A in complex with Rho-kinase inhibitors Y-27632, Fasudil, and H-1152P: structural bosis of selectivity. Structure (Comb) 2003;11:1595-607.
22. Wang P, Bitar KN. Rho A regulates sustained smooth muscle contraction through cytoskeletal reorganization of HSP27. Am J Physiol 1998;275:G1454-62.
23. Ibitayo AI, Sladick J, Tuteja S, et al. HSP27 in signal transduction and association with contractile proteins in smooth muscle cells. Am J Physiol 1999;277:G445-54.
24. Yamada H, Strahler J, Welsh MJ, et al. Activation of MAP kinase and translocation with HSP27 in bombesin-induced contraction of rectosigmoid smooth muscle. Am J Physiol 1995;269:G683-91.
25. Ott C, Iwanciw D, Graness A, et al. Modulation of the expression of connective tissue growth factor by alterations of the cytoskeleton. J Biol Chem 2003;278:44305-11.
26. Gnad R, Kaina B, Fritz G. Rho GTPases are involved in the regulation of NF-kappaB by genotoxic stress. Exp Cell Res 2001;264:244-9.
27. Segain JP, Raingeard de la Bletiere D, Sauzeau V, et al. Rho kinase blockade prevents inflammation via nuclear factor kappa B inhibition: evidence in Crohn's disease and experimental colitis. Gastroenterology 2003;124:1180-7.
28. Igarashi A, Nashiro K, Kikuchi K, et al. Significant correlation between connective tissue growth factor gene expression and skin sclerosis in tissue sections from patients with systemic sclerosis. J Invest Dermotol 1995;105:280-4.
29. Blom IE, Goldschmeding R, Leask A. Gene regulation of connective tissue growth factor: new targets for antifibrotic therapy? Matrix Biol 2002;21:473-82.
30. Abraham DJ, Shiwen X, Black CM, et al. Tumor necrosis factor alpha suppresses the induction of connective tissue growth factor by transforming growth factor-beta in normal and scleroderma fibroblasts. J Biol Chem 2000;275:15220-5.
31. Frazier K, Williams S, Kothapalli D, et al. Stimulation of fibroblast cell growth, matrix production, and granulation tissue formation by connective tissue growth factor. J Invest Dermotol 1996;107:404-11.
32. Riento K, Guasch RM, Garg R, et al. RhoE binds to ROCK I and inhibits downstream signaling. Mol Cell Biol 2003;23:4219-29.