Improvement in production and purification bioprocesses of bacterially expressed anti-alphaIIbbeta3 human single-chain FV antibodies

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 818, Issue 1 SPEC. ISS., 2005, Pages 43-51

  Robert, R ^a   Noubhani, A M ^b   Jacobin, Marie Josée ^a   Santarelli, X ^b   Clofent Sanchez, G ^a  

^a CNRS University Bordeaux Segalen   (France)

^b UNIVERSITÉ DE BORDEAUX   (France)

Author keywords

AlphaIIbbeta3 integrin; Fermentation; IMAC purification; Single chain Fv antibody fragment

Indexed keywords

AFFINITY CHROMATOGRAPHY; ANTIBODIES; BACTERIA; CYTOLOGY; ELECTROPHORESIS; FERMENTATION; GELS; GLUCOSE;

COMBINATORIAL LIBRARIES; CORONARY SYNDROMES; GEL ELECTROPHORESIS; ION AFFINITY COLUMNS;

PURIFICATION;

ALPHA IIB BETA3 INTEGRIN; CELL EXTRACT; GLUCOSE; IMMUNOGLOBULIN G ANTIBODY; INTEGRIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; SUCROSE; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTIBODY PRODUCTION; CONFERENCE PAPER; CONTROLLED STUDY; CULTURE MEDIUM; CYTOMETRY; DRUG ACTIVITY; DRUG POTENCY; DRUG SCREENING; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FED BATCH CULTURE; FED BATCH FERMENTATION; GENE EXPRESSION SYSTEM; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; IN VIVO STUDY; MALE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN INDUCTION; PROTEIN PROCESSING; PROTEIN PURIFICATION; RABBIT;

ANIMALIA;

EID: 13844256515     PISSN: 15700232     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jchromb.2004.10.038     Document Type: Conference Paper

References (28)

1. P.J. Hudson Curr. Opin. Biotechnol. 9 1998 395
2. M.J. Jacobin, J. Laroche-Traineau, M. Little, A. Keller, K. Peter, M. Welschof, A. Nurden, and G. Clofent-Sanchez J. Immunol. 168 2002 2035
3. E.J. Topol, T.V. Byzova, and E.F. Plow Lancet 353 1999 227
4. J.R. Swartz Curr. Opin. Biotechnol. 12 2001 195
5. U. Horn, W. Strittmatter, A. Krebber, U. Knupfer, M. Kujau, R. Wenderoth, K. Muller, S. Matzku, A. Pluckthun, and D. Riesenberg Appl. Microbiol. Biotechnol. 46 1996 52
6. D. Missiakas, and S. Raina J. Bacteriol. 179 1997 2465
7. S. Biocca, F. Ruberti, M. Tafani, P. Pierandrei-Amaldi, and A. Cattaneo Biotechnology (NY) 13 1995 1110
8. P. Jurado, D. Ritz, J. Beckwith, V. de Lorenzo, and L.A. Fernandez J. Mol. Biol. 320 2002 1
9. P. Martineau, and J.M. Betton J. Mol. Biol. 292 1999 921
10. M. Ayala, R.F. Balint, L. Fernandez-de-Cossio, J.W. Canaan-Haden, J.W. Larrick, and J.V. Gavilondo Biotechniques 18 1995 832
11. S.M. Kipriyanov, and M. Little Mol. Biotechnol. 12 1999 173
12. S.M. Kipriyanov, G. Moldenhauer, and M. Little J. Immunol. Methods 200 1997 69
13. J. Porath Protein Expr. Purif. 3 1992 263
14. M.M. Bradford Anal. Biochem. 72 1976 248
15. U.K. Laemmli Nature 227 1970 680
16. M. Dabadie, N. Valli, M.J. Jacobin, J. Laroche-Traineau, J.L. Barat, D. Ducassou, A.T. Nurden, and G. Clofent-Sanchez Platelets 12 2001 395
17. P. Martineau, P. Jones, and G. Winter J. Mol. Biol. 280 1998 117
18. S.H. Kim, D.G. Schindler, A.B. Lindner, D.S. Tawfik, and Z. Eshhar Mol. Immunol. 34 1997 891
19. R. Casalvilla, M. Duenas, M. Ayala, S. Cruz, L. Cruz, W.A. Buurman, and J.V. Gavilondo J. Biotechnol. 72 1999 1
20. L. Sanchez, M. Ayala, F. Freyre, I. Pedroso, H. Bell, V. Falcon, and J.V. Gavilondo J. Biotechnol. 72 1999 13
21. J.A. Moss, A.R. Coyle, J.M. Ahn, M.M. Meijler, J. Offer, and K.D. Janda J. Immunol. Methods 281 2003 143
22. C. Chothia, J. Novotny, R. Bruccoleri, and M. Karplus J. Mol. Biol. 186 1985 651
23. S. Rudikoff, and J.G. Pumphrey Proc. Natl. Acad. Sci. U.S.A. 83 1986 7875
24. K. Proba, A. Worn, A. Honegger, and A. Pluckthun J. Mol. Biol. 275 1998 245
25. K. Proba, A. Honegger, and A. Pluckthun J. Mol. Biol. 265 1997 161
26. K. Proba, L. Ge, and A. Pluckthun Gene 159 1995 203
27. A. Worn, and A. Pluckthun FEBS Lett. 427 1998 357
28. P.H. Bessette, F. Aslund, J. Beckwith, and G. Georgiou Proc. Natl. Acad. Sci. U.S.A. 96 1999 13703