High cytoplasmic expression in E. coli, purification, and in vitro refolding of a single chain Fv antibody fragment against the hepatitis B surface antigen

Journal of Biotechnology

Volumn 72, Issue 1-2, 1999, Pages 13-20

  Sánchez, Leticia ^a   Ayala, Marta ^a   Freyre, Freya ^a   Pedroso, Idolka ^a   Bell, Hansell ^a   Falcón, Viviana ^a   Gavilondo, Jorge V ^a  

^a CENTER FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Cuba)

Author keywords

Escherichia coli; Hepatitis B surface antigen; Recombinant antibody fragments; Single chain Fv antibody fragment

Indexed keywords

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; ANIMAL CELL CULTURE; ANTIBODIES; ANTIGENS; BIOASSAY; DENSITY (OPTICAL); ESCHERICHIA COLI; EXTRACTION; SOLUBILITY;

ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA); RECOMBINANT ANTIBODY FRAGMENT;

GENETIC ENGINEERING;

HEPATITIS B ANTIBODY; HEPATITIS B SURFACE ANTIGEN; HYBRID PROTEIN; INTERLEUKIN 2;

ANTIBODY PRODUCTION; ANTIGEN BINDING; ARTICLE; CYTOCHEMISTRY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; IN VITRO STUDY; MOLECULAR CLONING; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN STRUCTURE; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; HEPATITIS B SURFACE ANTIGENS; HUMANS; IMMUNOGLOBULIN VARIABLE REGION; MICROSCOPY, ELECTRON; MOLECULAR SEQUENCE DATA; PROTEIN FOLDING; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0345712326     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(99)00036-X     Document Type: Article

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