Differentiation of bacterial strains by thermal gradient gel electrophoresis using non-GC-Clamped PCR primers for the 16S-23S rDNA intergenic spacer region

FEMS Microbiology Letters

Volumn 243, Issue 1, 2005, Pages 235-242

  Yasuda, Michie ^a   Shiaris, Michael Peter ^a  

^a UNIVERSITY OF MASSACHUSETTS BOSTON   (United States)

Author keywords

16S 23S rDNA intergenic spacer region; Microbial diversity; Strain typing; TGGE

Indexed keywords

RIBOSOME DNA; SPACER DNA; TRANSFER RNA;

ANALYTIC METHOD; ARTICLE; ARTIFACT; BACTERIAL STRAIN; CONTROLLED STUDY; DNA DETERMINATION; DNA FINGERPRINTING; GC RICH SEQUENCE; GEL ELECTROPHORESIS; GENE AMPLIFICATION; GENE LOCATION; MELTING POINT; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SPECIES; STRAIN DIFFERENCE; TEMPERATURE; THERMAL GRADIENT GEL ELECTROPHORESIS;

BACTERIAL TYPING TECHNIQUES; DNA FINGERPRINTING; DNA PRIMERS; DNA, BACTERIAL; DNA, RIBOSOMAL SPACER; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GC RICH SEQUENCE; GRAM-NEGATIVE BACTERIA; HEAT; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; RNA, RIBOSOMAL, 23S; SEQUENCE ANALYSIS, DNA;

BACTERIA (MICROORGANISMS);

EID: 12844255932     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.femsle.2004.12.011     Document Type: Article

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