Use of homoduplex ribosomal DNA spacer amplification products and heteroduplex cross-hybridization products in the identification of Salmonella serovars

Applied and Environmental Microbiology

Volumn 62, Issue 8, 1996, Pages 2741-2746

  Jensen, Mark A ^a   Hubenr, Romeo J ^a  

^a DUPONT COMPANY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RIBOSOME DNA;

ARTICLE; BACTERIUM IDENTIFICATION; DNA SEQUENCE; DNA STRUCTURE; ELECTROPHORESIS; ELECTROPHORETIC MOBILITY; GENE AMPLIFICATION; GENETIC POLYMORPHISM; HYBRIDIZATION; NONHUMAN; SALMONELLA;

EID: 0029778799     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.62.8.2741-2746.1996     Document Type: Article

References (11)

1. Brosius, J., T. J. Dull, D. D. SIceter, and II. F. Noller. 1981. Gene organization and primary structure of a ribosomal RNA opcron from Esclicrichia coli. J. Mol. Biol. 148:107-127.
2. Dams, E., L. Ilendriks, Y. Van dc Peer, J. Neefs, G. Smits, I. Vandenbcmpt, and R. De Wächter. 1988. Compilation of small ribosomal subunit RNA sequences. Nucleic Acids Res. 16(Suppl.):r87-r171.
3. Frankel, D. S. 1984. Pattern recognition of Fourier transform infrared spectra of organic compounds. Anal. Chem. 56:1011-1014.
4. Grimont, F., and P. A. D. Grimont. 1986. Ribosomal ribonucleic acid gene restriction patterns as potential taxonomie tools. Ann. Inst. Pasteur Micro- biol. 1370:165-175.
5. Gutell, R. R., and G. E. Fox. 1988. A compilation of large subunit RNA sequences presented in a structural format. Nucleic Acids Res. 16(Suppl.): r175-r201.
6. Hubner, R. J. December 1989. U.S. patent 4,885,692.
7. Jensen, M. A., and N. Straus. 1993. Effect of PCR conditions on the formation of heteroduplex and single-stranded DNA products in the amplification of bacterial ribosomal DNA spacer regions. PCR Methods Applic. 3:186-194.
8. Jensen, M. A., J. A. Webster, and N. Straus. 1993. Rapid identification of bacteria on the basis of polymerasc chain reaction-amplified ribosomal DNA spacer polymorphisms. Appl. Environ. Microbiol. 59:945-952.
9. Vcrsalovic, J., T. Kocth, and J. R. Lupski. 1991. Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res. 19:6823-6831.
10. Webster, J. A. January 1988. U.S. patent 4,717,653.
11. Welsh, J., and M. McClelland. 1991. Genomic fingerprints produced by polymerase chain reaction with consensus tRNA gene primers. Nucleic Acids Res. 19:861-866.