A single band does not always represent single bacterial strains in denaturing gradient gel electrophoresis analysis

Biotechnology Letters

Volumn 23, Issue 15, 2001, Pages 1205-1208

  Sekiguchi, Hiroyuki ^a,b   Tomioka, Noriko ^a   Nakahara, Tadaatsu ^b   Uchiyama, Hiroo ^a  

^a NATIONAL INSTITUTE FOR ENVIRONMENTAL STUDIES   (Japan)

^b UNIVERSITY OF TSUKUBA   (Japan)

Author keywords

16S rDNA; Bias; DGGE; Microbial community; PCR

Indexed keywords

DNA;

ARTICLE; BACTERIUM IDENTIFICATION; COMMUNITY; CONTROLLED STUDY; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA LIBRARY; DNA SEQUENCE; MOLECULAR CLONING; NONHUMAN; PHYLOGENY; POLYMERASE CHAIN REACTION;

EID: 0034857157     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1010517117046     Document Type: Article

References (13)

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9. Nearly all single base substitutions in DNA fragments joined to a GC-clump can be detected by denaturing gradient gel electrophoresis
10. The neighbor-joining method: A new method for reconstructing phylogenetic trees
11. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice
12. Molecular detection, isolation, and physiological characterization of functionally dominant phenol-degrading bacteria in activated sludge
13. Molecular characterization of bacterial populations in petroleum-contaminated groundwater discharged from underground crude oil storage cavities