Modified electroporation protocol for Lactobacilli isolated from the chicken crop facilitates transformation and the use of a genetic tool

Journal of Microbiological Methods

Volumn 60, Issue 3, 2005, Pages 353-363

  Mason, Charlene K ^a   Collins, Martin A ^a,b   Thompson, Keith ^b  

^a QUEEN'S UNIVERSITY BELFAST   (United Kingdom)

^b Newforge Ln Belfast BT9 5PX N ^*  (Ireland)

Author keywords

Chicken crop; Electroporation; Lactobacillus; pG +host5:ISSI

Indexed keywords

PLASMID VECTOR;

ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM ISOLATE; CHICKEN; CHROMOSOME; CONTROLLED STUDY; CULTURE MEDIUM; DNA MODIFICATION; ELECTROPORATION; GENETIC PROCEDURES; GENETIC TRANSFORMATION; INCUBATION TIME; LACTOBACILLUS; LACTOBACILLUS SALIVARIUS; NONHUMAN; PRIORITY JOURNAL; TEMPERATURE SENSITIVITY;

ANIMALS; CHICKENS; CROP, AVIAN; CULTURE MEDIA; DNA TRANSPOSABLE ELEMENTS; ELECTROPHORESIS, AGAR GEL; ELECTROPORATION; GLYCINE; LACTOBACILLUS; PLASMIDS; PROBIOTICS; TRANSFORMATION, GENETIC;

BACTERIA (MICROORGANISMS); GALLUS GALLUS; LACTOBACILLUS; LACTOBACILLUS SALIVARIUS;

EID: 11844304162     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2004.10.013     Document Type: Article

References (34)

1. F.E. Ahmed Genetically modified probiotics in foods Trends Biotechnol. 21 2003 491 497
2. T.W. Aukrust, M.B. Brurberg, and I.F. Nes Transformation of Lactobacillus by electroporation J.A. Nickoloff Electroporation Protocols for Microorganisms Methods in Molecular Biology 1995 Humana Press New Jersey 201 208
3. M.T. Aymerich, M. Hugas, M. Garriga, R.F. Vogel, and J.M. Montfort Electrotransformation of meat lactobacilli. Effect of several parameters on their efficiency of transformation J. Appl. Bacteriol. 75 1993 320 325
4. S.S. Beasley, T.M. Takala, J. Reunanen, J. Apajalahti, and P.E.J. Saris Characterization and electrotransformation of Lactobacillus crispatus isolated from chicken crop and intestine Poultry Sci. 83 2004 45 48
5. T. Bhowmik, and J.L. Steele Development of an electroporation procedure for gene disruption in Lactobacillus helveticus CNRZ32 J. Gen. Microbiol. 139 1993 1433 1439
6. I. Biswas, A. Gruss, S.D. Ehrlich, and E. Maguin High-efficiency gene inactivation and replacement system for Gram-positive bacteria J. Bacteriol. 175 1993 3628 3635
7. B.M. Chassy, and J.L. Flickinger Transformation of Lactobacillus casei by electroporation FEMS Microbiol. Lett. 44 1987 173 177
8. W.M. de Vos Gene cloning and expression in lactic streptococci FEMS Microbiol. Rev. 46 1987 281 295
9. S. Edelman, B. Westerlund-Wikström, S. Leskelä, H. Kettunen, N. Rautonen, J. Apajalahti, and T.K. Korhonen In vitro adhesion specificity of indigenous Lactobacilli within the avian intestinal tract Appl. Environ. Microbiol. 68 2002 5155 5159
10. L. Gory, M.-C. Montel, and M. Zagorec Use of green fluorescent protein to monitor Lactobacillus sakei in fermented meat products FEMS Microbiol. Lett. 194 2001 127 133
11. L.L. Guan, K.E. Hagen, G.W. Tannock, D.R. Korver, G.M. Fasenko, and G.E. Allison Detection and identification of Lactobacillus species in crops of broilers of different ages by using PCR-denaturing gradient gel electrophoresis and amplified ribosomal DNA restriction analysis Appl. Environ. Microbiol. 69 2003 6750 6757
12. W. Hammes, K.H. Schleifer, and O. Kandler Mode of action of glycine on the biosynthesis of peptidoglycan J. Bacteriol. 116 1973 1029 1053
13. H.G.H.J. Heilig, E.G. Zoetendal, E.E. Vaughan, P. Marteau, A.D.L. Akkermans, and W.M. de Vos Molecular diversity of Lactobacillus spp. and other lactic acid bacteria in the human intestine as determined by specific amplification of 16S ribosomal DNA Appl. Environ. Microbiol. 68 2002 114 123
14. N.C.K. Heng, J.M. Bateup, D.M. Loach, X. Wu, H.F. Jenkinson, M. Morrison, and G.W. Tannock Influence of different functional elements of plasmid pGT232 on maintenance of recombinant plasmids in Lactobacillus reuteri populations in vitro and in vivo Appl. Environ. Microbiol. 65 1999 5378 5385
15. T. Klaenhammer, E. Altermann, F. Arigoni, A. Bolotin, F. Breidt, and J. Broadbent Discovering lactic acid bacteria by genomics Antonie van Leeuwenhoek 82 2002 29 58
16. J.R. Klein, C. Ulrich, and R. Plapp Characterization and sequence analysis of a small cryptic plasmid from Lactobacillus curvatus LTH683 and its use for construction of new Lactobacillus cloning vectors Plasmid 30 1993 14 29
17. E. Maguin, H. Prevost, S.D. Ehrlich, and S.D. Gruss Efficient insertional mutagenesis in lactococci and other Gram-positive bacteria J. Bacteriol. 178 1996 931 935
18. Mason, C.K., 2003. Investigation of the probiotic potential of bacteria isolated from chicken crops with respect to cellular adherence in vitro. PhD thesis. Queens University, Belfast.
19. D.A. Mills Mutagenesis in the post genomics era: tools for generating insertional mutations in the lactic acid bacteria Curr. Opin. Biotechnol. 12 2001 503 509
20. T. Neu, and B. Henrich New thermosensitive delivery vector and its use to enable nisin-controlled gene expression in Lactobacillus gasseri Appl. Environ. Microbiol. 69 2003 1377 1382
21. M. Posno, R.J. Leer, N. van Luijk, M.J.F. van Giezen, P.T.H.M. Heuvelmans, B.C. Lokman, and P.H. Pouwels Incompatability of Lactobacillus vectors with replicons derived from small cryptic Lactobacillus plasmids and segregational instability of the introduced vectors Appl. Environ. Microbiol. 57 1991 1822 1828
22. P. Renault Genetically modified lactic acid bacteria: applications to food or health and risk assessment Biochemie 84 2002 1073 1087
23. W.M. Russell, and T.R. Klaenhammer Efficient system for directed integration into the Lactobacillus acidophilus and Lactobacillus gasseri chromosomes via homologous recombination Appl. Environ. Microbiol. 67 2001 4361 4364
24. J. Sambrook, E.F. Fritsch, and T. Maniatis Molecular Cloning: A Laboratory Manual 2nd Edition 1989 Cold Spring Harbor Laboratory Cold Spring Harbor, New York
25. G. Schmidt, C. Hertel, and W.P. Hammes Molecular characterisation of the dnaK operon of Lactobacillus sakei LTH681 Syst. Appl. Microbiol. 22 1999 321 328
26. P. Serror, T. Sasaki, S.D. Ehrlich, and E. Maguin Electrotransformation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis with various plasmids Appl. Environ. Microbiol. 68 2002 46 52
27. P. Serror, G. Ilami, H. Chouayekh, S.D. Ehrlich, and E. Maguin Transposition in Lactobacillus delbrueckii subsp. bulgaricus: identification of two thermosensitive replicons and two functional insertion sequences Microbiology 149 2003 1503 1511
28. D. Simon, and A. Chopin Construction of a vector plasmid family and its use for molecular cloning in Streptococcus lactis Biochimie 70 1988 559 566
29. +host5 into Lactobacillus plantarum NCFB1193. Diplomarbeit, Technische Universität München.
30. K. Thompson, and M.A. Collins Improvement in electroporation efficiency for Lactobacillus plantarum by the inclusion of high concentrations of glycine in the growth medium J. Microbiol. Methods 26 1996 73 79
31. D.C. Walker, and T.R. Klaenhammer Isolation of a novel IS3 group insertion element and construction of an integration vector for Lactobacillus spp J. Bacteriol. 176 1994 5330 5340
32. D.C. Walker, K. Aoyama, and T.R. Klaenhammer Electrotransformation of Lactobacillus acidophilus Group A1 FEMS Microbiol. Lett. 138 1996 233 237
33. F. Xu, L.E. Pearce, and P.-L. Yu Construction of a family of Lactococcal vectors for gene cloning and translational fusion FEMS Microbiol. Lett. 77 1991 55 59
34. A. Zink, J.R. Klein, and R. Plapp Transformation of Lactobacillus delbruckii subsp. lactis by electroporation and cloning of origins of replication by use of a positive selection vector FEMS Microbiol. Lett. 78 1991 207 212