Molecular diversity of Lactobacillus spp. and other lactic acid bacteria in the human intestine as determined by specific amplification of 16S ribosomal DNA

Applied and Environmental Microbiology

Volumn 68, Issue 1, 2002, Pages 114-123

  Heilig, H G H J ^a   Zoetendal, E G ^a   Vaughan, E E ^a   Marteau, P ^a   Akkermans, A D L ^a   De Vos, W M ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

MOLECULAR DIVERSITY;

BACTERIA; DNA; ELECTROPHORESIS; FOOD PRODUCTS;

MICROBIOLOGY;

RIBOSOME DNA;

BACTERIUM;

ARTICLE; DENATURING GRADIENT GEL ELECTROPHORESIS; ECOSYSTEM; FEMALE; GASTROINTESTINAL TRACT; GENE AMPLIFICATION; HUMAN; INTESTINE FLORA; LACTIC ACID BACTERIUM; LACTOBACILLUS; MALE; MOLECULAR BIOLOGY; NUCLEOTIDE SEQUENCE; PHYLOGENY; POLYMERASE CHAIN REACTION;

ADULT; AGING; BACTERIA, ANAEROBIC; DNA PRIMERS; DNA, RIBOSOMAL; ECOSYSTEM; ELECTROPHORESIS; HUMANS; INFANT; INFANT, NEWBORN; INTESTINES; LACTOBACILLUS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; PROBIOTICS; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA; VARIATION (GENETICS);

BACTERIA (MICROORGANISMS); LACTOBACILLUS; LACTOBACILLUS RUMINIS; LEUCONOSTOC; PEDIOCOCCUS; POSIBACTERIA; WEISSELLA;

EID: 0036139245     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.68.1.114-123.2002     Document Type: Article

References (50)

1. The normal Lactobacillus flora of healthy human rectal and oral mucosa
2. The oligonucleotide probe database
3. Rapid PCR-based procedure to identify lactic acid bacteria: Application to six common Lactobacillus species
4. Phylogenetic analysis of the genus Lactobacillus and related lactic acid bacteria as determined by reverse transcriptase sequencing of 16S rRNA
5. A DNA probe for Lactobacillus delbruecteckeii
6. Creating and maintaining the gastrointestinal ecosystem: What we know and need to know from gnotobiology
7. Normal indigenous intestinal flora
8. Colonic metabolism of wheat starch in health of humans. Effects on fecal outputs and clinical symptoms
9. Lactic acid bacteria in meat fermentation
10. The genus Lactobacillus
11. A 16S rRNA-targeted probe for detection of lactobacilli and enterococci in fecal samples by fluorescent in situ hybridization
12. Development of diagnostic oligonucleotide probes for four Lactobacillus species occurring in the intestinal tract
13. 23S rRNA-targeted oligonucleotide probes for rapid identification of meat lactobacilli
14. Molecular analysis of commensal host-microbial relationships in the intestine
15. Adherence of probiotic bacteria to human intestinal mucus in healthy infants and during rotavirus infection
16. Monitoring the enrichment and isolation of sulfate reducing bacteria by using oligonucleotide hybridization probes designed from environmentally derived 16S rRNA sequences
17. Analysis of fecal populations of bifidobacteria and lactobacilli and investigations of the immunological responses of their human hosts to the predominant strains
18. 16S/23S rRNA sequencing
19. Quantitative fluorescence in situ hybridization of Bifidobacterium spp. with genus-specific 16S rRNA targeted probe and its application in fecal samples
20. Developmental microbial ecology of the neonatal gastrointestinal tract
21. The RDP (ribosomal database project)
22. Digestibility and bulking effect of ispaghula husks in healthy humans
23. Comparative study of bacterial groups within the human cecal and fecal microbiota
24. Probiotics: Towards demonstrating efficacy
25. Molecular analysis of the composition of the bifidobacterial and Lactobacillus microflora of humans
26. The human gastrointestinal tract
27. Human fecal flora: The normal flora of 20 Japanese-Hawaiians
28. Multiplex PCR for the detection of Lactobacilluspontis and two related species in a sourdough fermentation
29. Profiling of complex populations by denaturing gradient gel electrophoresis of polymerase chain reaction-amplified genes coding for 16S rRNA
30. Isolation of tannin-degrading lactobacilli from humans and fermented foods
31. Isolation of a DNA probe for Lactobacillus curvatus
32. DNA probes for the detection of Lactobacillus helveticus
33. Pyxigraphic sampling to enumerate methanogens and anaerobes in the right colon of healthy humans
34. Clinical uses of probiotics for stabilizing the gut mucosal barrier: Successful strains and future challenges
35. Rapid silver staining and recovery of PCR products separated on polyacrylamide gels
36. Bifidobacterial diversity in human feces detected by genus-specific PCR and denaturing gradient gel electrophoresis
37. Phylogenetic relationships of lactic acid bacteria
38. Design and evaluation of a Lactobacillus group-specific ribosomal RNA-targeted hybridization probe and its application to the study of intestinal microecology in pigs
39. Quantification of bacterial groups within the human fecal flora by oligonucleotide probe hybridization
40. Denaturing gradient gel electrophoresis analysis of 16S ribosomal DNA amplicons to monitor changes in fecal bacterial populations of weaning pigs after introduction of Lactobacillus reuteri strain MM53
41. Direct analysis of genes encoding 16S rRNA from complex communities reveals many novel molecular species within the human gut
42. The microecology of lactobacilli inhabiting the gastrointestinal tract
43. Identification of Lactobacillus isolates from the gastrointestinal tract, silage, and yoghurt by 16S-23S rRNA gene intergenic spacer region sequence comparisons
44. Analysis of the intestinal microflora: A renaissance
45. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice
46. Molecular approaches to study probiotic bacteria
47. A molecular view of the intestinal ecosystem
48. Temperature gradient gel electrophoresis analysis of 16S rRNA from human fecal samples reveals stable and host-specific communities of active bacteria
49. Molecular characterisation of microbial communities based on 16S rRNA sequence diversity