Improvement in electroporation efficiency for Lactobacillus plantarum by the inclusion of high concentrations of glycine in the growth medium

Journal of Microbiological Methods

Volumn 26, Issue 1-2, 1996, Pages 73-79

  Thompson, Keith ^a   Collins, Martin A ^b  

^a AGRI FOOD AND BIOSCIENCES INSTITUTE   (United Kingdom)

^b QUEEN'S UNIVERSITY BELFAST   (United Kingdom)

Author keywords

Electroporation; Lactobacillus plantarum; Transformation

Indexed keywords

GLYCINE;

ARTICLE; CELL TRANSFORMATION; CONTROLLED STUDY; CULTURE MEDIUM; ELECTROPORATION; LACTOBACILLUS PLANTARUM; NONHUMAN; PLASMID; PRIORITY JOURNAL;

LACTOBACILLUS PLANTARUM;

EID: 0030200792     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/0167-7012(96)00845-7     Document Type: Article

References (14)

1. [1] Mercenier, A., Pouwels, P.H. and Chassy, B.M. (1994) Genetic engineering of lactobacilli, leuconostocs and Streptococcus thermophilus. In: Genetics and Biotechnology of Lactic Acid Bacteria (Eds. M.J. Gasson and W.M. de Vos). Blackie, Glasgow, pp. 252-293.
2. [2] Aukrust, T. and Nes, I.F. (1988) Transformation of Lactobacillus plantarum with the plasmid pTV1 by electroporation. FEMS Microbiol. Lett. 52, 127-132.
3. [3] Aukrust, T. and Blom, H. (1992) Transformation of Lactobacillus strains used in meat and vegetable fermentations. Food Res. Int. 25, 253-261.
4. [4] Aymerich, M.T., Hugas, M., Garriga, M., Vogel, R.F. and Montfort, J.M. (1993) Electrotransformation of meat lactobacilli. Effect of several parameters on their efficiency of transformation. J. Appl. Bacteriol. 75, 320-325.
5. [5] Wei, M.-Q., Rush, C.M., Norman, J.M., Hafner, L.M., Epping, R.J. and Timms, P. (1995) An improved method for the transformation of Lactobacillus strains using electroporation. J. Microbiol. Methods 21, 97-109.
6. [6] Posno, M., Heuvelmans, P.T.H.M., van Giezen, M.J.F., Lokman, B.C., Leer, R.L. and Pouwels, P.M. (1991) Complementation of the instability of Lactobacillus strains to utilize D-xylose with D-xylose catabolism-encoding genes of Lactobacillus pentosus. Appl. Environ. Microbiol. 57, 2764-2766.
7. [7] Badii, R., Jones, S. and Warner, P.J. (1989) Sphaeroplast and electroporation-mediated transformation of Lactobacillus plantarum. Lett. Appl. Microbiol. 9, 41-44.
8. [8] Trevors, J.T., Chassy, B.M., Dower, W.J. and Blaschek, H.P. (1992) Electrotransformation of bacteria by plasmid DNA. In: Guide to Electroporation and Electrofusion (Eds. D.C. Chang, B.M. Chassy, J.A. Saunders and A.E. Sowers). Academic Press, New York, pp. 265-290.
9. [9] Holo, H. and Nes, I.F. (1989) High-frequency transformation, by electroporation, of Lactococcus lactis subsp. cremoris grown with glycine in osmotically stabilized media. Appl. Environ. Microbiol. 55, 3119-3123.
10. [10] Wells, J.M., Wilson, P.W. and LePage, R.W.F. (1993) Improved cloning vectors and transformation procedure for Lactococcus lactis. J. Appl. Bacteriol. 74, 629-636.
11. [11] Posno, M., Leer, R.J. van Luijk, N., van Giezen, M.J.F., Heuvelmans, P.T.H.M., Lokman, B.C. and Pouwels, P.H. (1991) Incompatibility of Lactobacillus vectors with replicons derived from small cryptic Lactobacillus plasmids and segregational instability of the introduced vectors. Appl. Environ. Microbiol. 57, 1822-1828.
12. [12] Anderson, D.G. and McKay, L.L. (1983) Simple and rapid method for isolating large plasmid DNA from lactic streptococci. Appl. Environ. Microbiol. 46, 549-552.
13. [13] Luchansky, J.B., Muriana, P.M. and Klaenhammer, T.R. (1988) Application of electroporation for transfer of plasmid DNA to Lactobacillus, Lactococcus, Leuconostoc, Listeria, Pediococcus, Bacillus, Staphylococcus, Enterococcus, and Propionibacterium. Mol. Microbiol. 2, 637-646.
14. [14] Bhowmik, T. and Steele, J.L. (1993) Development of an electroporation procedure for gene disruption in Lactobacillus helveticus CNRZ32. J. Gen. Microbiol. 139, 1433-1439.