Expression and purification of recombinant human angiopoietin-2 produced in Chinese hamster ovary cells

Protein Expression and Purification

Volumn 39, Issue 2, 2005, Pages 175-183

  Hwang, Su Jeong ^a   Choi, Han Ho ^a   Kim, Kyung Tae ^a   Hong, Hyo Jeong ^b   Koh, Gou Young ^a   Lee, Gyun Min ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^b Aprogen Inc   (South Korea)

Author keywords

Angiopoietin; Chinese hamster ovary cells; FLAG tag; Tie2 receptor

Indexed keywords

ANGIOPOIETIN 2; DIHYDROFOLATE REDUCTASE; METHOTREXATE; RECOMBINANT PROTEIN; TIE 1 RECEPTOR;

ANIMAL; ARTICLE; CELL SURVIVAL; CHO CELL; CRICETULUS; CULTURE MEDIUM; DOSE RESPONSE; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE AMPLIFICATION; GENE VECTOR; GENETIC TRANSFECTION; GENETICS; HAMSTER; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; WESTERN BLOTTING;

ANGIOPOIETIN-2; ANIMALS; BLOTTING, WESTERN; CELL SURVIVAL; CHO CELLS; CRICETINAE; CRICETULUS; CULTURE MEDIA, CONDITIONED; CULTURE MEDIA, SERUM-FREE; DOSE-RESPONSE RELATIONSHIP, DRUG; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; GENE AMPLIFICATION; GENETIC VECTORS; HUMANS; METHOTREXATE; PLASMIDS; RECEPTOR, TIE-1; RECOMBINANT PROTEINS; TETRAHYDROFOLATE DEHYDROGENASE; TRANSFECTION;

CRICETINAE; CRICETULUS GRISEUS;

EID: 11844287623     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.09.005     Document Type: Article

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