Expression and purification of active recombinant ATM protein from transiently transfected mammalian cells

Protein Expression and Purification

Volumn 22, Issue 3, 2001, Pages 462-466

  Rhodes, Nelson ^a   Gilmer, Tona M ^a   Lansing, Timothy J ^a  

^a GLAXOSMITHKLINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ATM PROTEIN; CELL CYCLE; CONCENTRATION (PARAMETERS); CULTURE VESSEL; DNA REPAIR; EPITOPE; IMMUNOPRECIPITATION; PROTEIN KINASE; PROTEIN PURIFICATION; SDS POLYACRYLAMIDE GEL ELECTROPHORESIS; TRANSIENT TRANSFECTION;

ATAXIA; ATAXIA TELANGIECTASIA; MAMMALIA;

EID: 0034881177     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1459     Document Type: Article

References (34)

1. The genetic defect in ataxia telangiectasia
2. A single ataxia telangiectasia gene with a product similar to PI-3 kinase
3. A mammalian cell cycle checkpoint pathway utilizing p53 and GADD45 is defective in ataxia telangiectasia
4. Enhanced phosphorylation of p53 by ATM in response to DNA damage
5. Activation of ATM kinase by ionizing radiation and phosphorylation of p53
6. Ataxia telangiectasia mutant protein activates c-Ab1 tyrosine kinase in response to ionizing radiation
7. Requirement of ATM-dependent phosphorylation of brca1 in the DNA damage response to double-strand breaks
8. Role for ATM in DNA damage-induced phosphorylation of BRCA1
9. Inhibition of phosphoinositide 3-kinase related kinases by the radiosensitizing agent wortmannin
10. Participation of ATM in insulin signaling through phosphorylation of eIF-4E binding protein 1 (4EBP1)
11. Rapid ATM-dependent phosphorylation of MDM2 precedes p53 accumulation in response to DNA damage
12. Ataxia telangiectasia-mutated phosphorylates Chk2 in vivo and in vitro
13. Threonine 68 phosphorylation by ataxia telangiectasia mutated is required for efficient activation of Chk2 in response to ionizing radiation
14. ATM phosphorylates p95/nbs1 in an S-phase checkpoint pathway
15. ATM-dependent phosphorylation of nibrin in response to radiation exposure
16. Functional link between ataxia-telangiectasia and Nijmegen breakage syndrome gene products
17. ATM phosphorylation of Nijmegen breakage syndrome protein is required in a DNA damage response
18. Characterization of ATM expression, localization, and associated DNA-dependent protein kinase activity
19. Recombinant ATM protein complements the cellular A-T phenotype
20. Cloning and expression of the ataxia-telangiectasia gene in baculovirus
21. Purification and DNA binding properties of the ataxia-telangiectasia gene product ATM
22. Purification and characterization of ATM from human placenta
23. ATM binds to beta-adaptin in cytoplasmic vesicles
24. Transfecting mammalian cells: Optimization of critical parameters affecting calcium-phosphate precipitate formation
25. Analysis of mutation in human cells by using an Epstein-Barr virus shuttle system
26. Monoclonal antibodies generated against recombinant ATM support kinase activity
27. Substrate specificities and identification of putative substrates of ATM kinase family members
28. The complete sequence of the coding region of the ATM gene reveals similarity to cell cycle regulators in different species
29. The novel ATM-related protein TRRAP is an essential cofactor for the c-Myc and E2F oncoproteins
30. When is a lipid kinase not a lipid kinase? When it is a protein kinase
31. Fragments of ATM which have dominant-negative or complementing activity
32. Production of high-titer helper-free retroviruses by transient transfection
33. The DNA damage response: Putting checkpoints in perspective
34. The many substrates and functions of ATM