Characterization of a recombinant granzyme B derivative as a "restriction" protease

Protein Expression and Purification

Volumn 39, Issue 2, 2005, Pages 209-218

  Fynbo, Charlotte Harkjær ^a,b   Lorentsen, Rikke Høegh ^a,b   Etzerodt, Michael ^a,b   Thøgersen, Hans Christian ^a,b   Holtet, Thor Las ^b  

^a AARHUS UNIVERSITY   (Denmark)

^b Borean Pharma A/S   (Denmark)

Author keywords

Granzyme B; Hydrolytic activity; Proteolytic activity; Recombinant serine protease; Stability

Indexed keywords

GRANZYME; GZMB PROTEIN, HUMAN; HYBRID PROTEIN; PROTEINASE; SERINE PROTEINASE;

ARTICLE; COMPARATIVE STUDY; DIFFERENTIAL SCANNING CALORIMETRY; ENZYME ACTIVATION; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME SPECIFICITY; ENZYME STABILITY; ESCHERICHIA COLI; GEL CHROMATOGRAPHY; GENE VECTOR; GENETICS; HUMAN; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR CLONING; PH; POINT MUTATION; PROTEIN PROCESSING; PROTEIN RENATURATION; SITE DIRECTED MUTAGENESIS;

CALORIMETRY, DIFFERENTIAL SCANNING; CHROMATOGRAPHY, GEL; CLONING, MOLECULAR; ENDOPEPTIDASES; ENZYME ACTIVATION; ENZYME STABILITY; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; GENETIC VECTORS; GRANZYMES; HUMANS; HYDROGEN-ION CONCENTRATION; KINETICS; MUTAGENESIS, SITE-DIRECTED; POINT MUTATION; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN RENATURATION; RECOMBINANT FUSION PROTEINS; SERINE ENDOPEPTIDASES; SUBSTRATE SPECIFICITY;

ANIMALIA; BOVINAE; ESCHERICHIA COLI;

EID: 11844282754     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.10.010     Document Type: Article

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