High-throughput cytotoxicity screening: Hit and miss

Drug Discovery Today

Volumn 6, Issue SUPPL.1, 2001, Pages

  Crouch, Sharon P M ^a   Slater, Kevin J ^a  

^a LumiTech Ltd   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 10644291806     PISSN: 13596446     EISSN: None     Source Type: Journal    
DOI: 10.1016/s1359-6446(01)00151-9     Document Type: Review

References (29)

1. Panchaganula, R. and Thomas, N.S. (2000) Biopharmaccutics and pharmacokinetics in drug research. Int. J. Pharm. 201, 131- 150
2. Collins, M.A. et al. (1999) Driving drug discovery and patient therapy via the encapsulation and fusion of knowledge. Drug Des. Discov. 16, 181- 194
3. Pharmaceutical Industry Profile (2000) Appendix I, pp. 112- 137 (http://www.phrma.org/publications/publications/profile00/)
4. Todd, M.D. et al. (1999) Toxicity screening of a combinatorial library: correlation of cytotoxicity and gene induction to compound structure. J.Biomol.Screening 4, 259- 268
5. Johnson, D.E. and Wolfgang, G.H.I. (2000) Predicting human safety: screening and computational approaches. Drug Discov.Today 5, 445- 454
6. Valler, M.J. and Green, D. (2000) Diversity screening versus focussed screening in drug discovery. Drug Discov.Today 5, 286- 293
7. Petty, R.D. et al. (1995) Comparison of MTT and ATP-based assays for the measurement of viable cell number. J. Biolum. Chemilum. 10, 29- 34
8. Squarito, R.C. et al. (1995) Comparison of a novel redox dye cell growth assay to the ATP bioluminescence. Gynecol. Oncol. 58, 101- 105
9. Fox, S. et al. (1999) High throughput screening for drug discovery: continually transitioning into new technology. J. Biomol. Screening 4, 183- 186
10. Fox, S. et al. (1998) High throughput screening: strategies and suppliers. HighTech Business Decisions, Moraga, CA (http://www.hitechbiz.com/)
11. Hertzberg, R.P. and Pope, A.J. (2000) High-throughput screening: new technology for the 21st century. Curr. Opin. Chem. Biol. 4, 445- 451
12. Offin, P and Drake, R. (2000) The SelecT consortium. J.Assoc.Lab.Auto. 5, 20- 22
13. O'Brien, J. et al. (2000) Investigation of the Alamar Blue (resazurin) dye for the assessment of mammalian cell cytotoxicity. Eur. J. Biochem. 267, 5421- 5426
14. Sundberg, S.A. (2000) High-throughput and ultra-high-throughput screening: solution- and cell-based approaches. Curr. Opin. Biotechnol. 11, 47- 53
15. Society Updates (1999) SBS proposed microplate specification. J. Biomol. Screening 4, 167- 174
16. Gaines Das, R.E. and Meager, A. (1995) Evaluation of assay designs for assays using microtitre plates: results of a study of in vitro bioassays and immunoassays for tumour necrosis factor (TNF). Biologicals 23, 285- 297
17. Zhang, J.H. et al. (1999) A simple statisitical parameter for use in evaluation and validation of high throughput screening assays. J. Biomol. Screening 4, 67- 73
18. Rosenkranz, H.S. and Cunningham, A.R. (2000) The high production volume chemical challenge program: the relevance of the in vivo micronucleus assay. Regul.Toxicol.Pharmacol. 31, 182- 189
19. King, M.A. (2000) Detection of dead cells and measurement of cell killing by flow cytometry. J.Immunol.Methods 243, 155- 166
20. Ashcroft, R.G. and Lopez, PA. (2000) Commercial high speed machines open new opportunities in high throughput flow cytometry (HTFC). J.Immunol.Methods 243, 13- 24
21. Wodnicka, M. et al. (2000) Novel fluorescent technology platform for high throughput cytotoxicity and proliferation assays. J.Biomol.Screening 5, 141- 152
22. Crouch, S.P.M. et al. (1993) The use of ATP bioluminescence as a measure of cell proliferation and cytotoxicity. J. Immunol. Methods 160, 81- 88
23. Einsphar, J. et al. (1988) Pharmacological pitfalls in the use of MTT (versus human tumour clonogenic-HTCA) assay to quantitate chemosensitivity of human tumour cell lines. Proc. Am.Assoc. Cancer Res. 29, 492- 502
24. Colcy, H.M. et al. (1997) Chemosensitivity testing of fresh and continuous tumour cell cultures using lactate dehydrogenase. Anticancer Res. 17, 231- 236
25. Taylor, D.E. et al. (2000) Combining high information content and high capacity in a platform for drug discovery. In High throughput screening: the next generation (Dixon, G.K. et al., eds), pp. 43- 51, BIOS Scientific Publishers Ltd
26. Kain, S.R. (1999) Green fluorescent protein (GFP): applications in cell-based assays for drug discovery. Drug Discov.Today 4, 304- 312
27. Vermes, I. et al. (2000) Flow cytometry of apoptotic cell death. J. Immunol. Methods 243, 167- 190
28. Bradbury, D.A. et al. (2000) Measurement of the ADP: ATP ratio in human leukaemic cell lines can be used as an indicator of cell viability, necrosis and apoptosis. J. Immunol. Methods 240, 79- 92
29. Nuwaysir, E.F. et al. (1999) Microarrays and toxicology: the advent of toxicogenomics. Molecular Carcinog. 24, 153- 159