Detection of dead cells and measurement of cell killing by flow cytometry

Journal of Immunological Methods

Volumn 243, Issue 1-2, 2000, Pages 155-166

  King, Malcolm A ^a  

^a Pacific Laboratory Medicine Services   (Australia)

Author keywords

Cell killing; Cytotoxicity; Dead cells; Flow cytometry

Indexed keywords

CELL DEATH; CELL MEDIATED CYTOTOXICITY; CELL MEMBRANE; CELL STRAIN K 562; CYTOTOXICITY; DRUG CYTOTOXICITY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNOPHENOTYPING; LIGHT SCATTERING; PRIORITY JOURNAL; REVIEW;

EID: 0034699378     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(00)00232-5     Document Type: Article

References (63)

1. Aeschbacher M., Reinhardt C.A., Zbinden G. A rapid cell membrane permeability test using fluorescent dyes and flow cytometry. Cell Biol. Toxicol. 2:1986;247.
2. Bernal S.D., Shapiro H.M., Chen L.B. Monitoring the effect of anti-cancer drugs on L1210 cells by a mitochondrial probe, rhodamine-123. Int. J. Cancer. 30:1982;219.
3. Bhuyan B.K., Loughman B.E., Fraser T.J., Day K.J. Comparison of different methods of determining cell viability after exposure to cytotoxic compounds. Exp. Cell Res. 97:1976;275.
4. Bohn B. High-sensitivity cytofluorometric quantitation of lectin and hormone binding to surfaces of living cells. Exp. Cell Res. 103:1976;39.
5. 51Cr-labeled allogeneic target cells in vitro; inhibition by isoantibody and by drugs. Immunology. 14:1968;181.
6. Chang L., Gusewitch G.A., Chritton D.B.W., Folz J.C., Lebeck L.K., Nehlsen-Cannarella S.L. Rapid flow cytometric assay for the assessment of natural killer cell activity. J. Immunol. Methods. 166:1993;45.
7. Coder D.M. Assessment of cell viability. Current Protocols in Cytometry. 1997;9.2.1 Wiley, New York.
8. Dangl J.L., Parks D.R., Oi V.T., Herzenberg L.A. Rapid isolation of cloned isotype switch variants using fluorescence activated cell sorting. Cytometry. 2:1982;395.
9. Darzynkiewicz Z., Traganos F., Staiano-Coico L., Kapuscinski J., Melamed M.R. Interactions of rhodamine 123 with living cells studied by flow cytometry. Cancer Res. 42:1982;799.
10. De Clerck L.S., Bridts C.H., Mertens A.M., Moens M.M., Stevens W.J. Use of fluorescent dyes in the determination of adherence of human leucocytes to endothelial cells and the effect of fluorochromes on cellular function. J. Immunol. Methods. 172:1994;115.
11. Dive C., Cox H., Watson J.V., Workman P. Polar fluorescein derivatives as improved substrate probes for flow cytoenzymological assay of cellular esterases. Mol. Cell. Probes. 2:1988;131.
12. Dive C., Watson J.V., Workman P. Multiparametric analysis of cell membrane permeability by two colour flow cytometry with complementary fluorescent probes. Cytometry. 11:1990;244.
13. Ellis T.M. Flow cytometric assays of cell-mediated cytotoxicity. Bauer K.D., Duque R.E., Shankey T.V. Clinical Flow Cytometry Principles and Application. 1993;497 Williams and Wilkins, Baltimore.
14. Ellwart J.W., Kremer J.-P., Dormer P. Drug testing in established cell lines by flow cytometric vitality measurements versus clonogenic assay. Cancer Res. 48:1988;5722.
15. Flieger D., Gruber R., Schlimok G., Reiter C., Pantel K., Riethmuller G. A novel non-radioactive cellular cytotoxicity test based on the differential assessment of living and killed target and effector cells. J. Immunol. Methods. 180:1995;1.
16. Hamori E., Arndt-Jovin D.J., Grimwade B.G., Jovin T.M. Selection of viable cells with known DNA content. Cytometry. 1:1980;132.
17. Hatam L., Schuval S., Bonagura V.R. Flow cytometric analysis of natural killer cell function as a clinical assay. Cytometry. 16:1994;59.
18. Hollo Z., Homolya L., Hegedus T., Sarkadi B. Transport properties of the multidrug resistance-associated protein (MRP) in human tumour cells. FEBS Lett. 383:1996;99.
19. Homolya L., Hollo Z., Germann U.A., Pastan I., Gottesman M.M., Sarkadi B. Fluorescent cellular indicators are extruded by the multidrug resistance protein. J. Biol. Chem. 268:1993;21493.
20. Hubl W., Iturraspe J., Martinez G.A., Hutcheson C.E., Roberts C.G., Fisk D.D., Sugrue M.W., Wingard J.R., Braylan R.C. Measurement of absolute concentration and viability of CD34+ cells in cord blood and cord blood products using fluorescent beads and cyanine nucleic acid dyes. Cytometry (Comm. Clin. Cytometry). 34:1998;121.
21. Idziorek T., Estaquier J., De Bels F., Ameisen J.-C. YOPRO-1 permits cytofluorometric analysis of programmed cell death (apoptosis) without interfering with cell viability. J. Immunol. Methods. 185:1995;249.
22. Jacobs D.B., Pipho C. Use of propidium iodide staining and flow cyometry to measure antibody-mediated cytotoxicity: resolution of complement-sensitive and resistant target cells. J. Immunol. Methods. 62:1983;101.
23. Jewett A., Bonavida B. Target-induced inactivation and cell death by apoptosis in a subset of human NK cells. J. Immunol. 156:1996;907.
24. Johann S., Blumel G., Lipp M., Forster R. A versatile flow cytometry-based assay for the determination of short- and long-term natural killer cell activity. J. Immunol. Methods. 185:1995;209.
25. Jones J., Hallett M.B., Morgan B.P. Reversible cell damage by T-cell perforins. Biochem. J. 267:1990;303.
26. Jonsson B., Liminga G., Csoka K., Fridborg H., Dhar S., Nygren P., Larsson R. Cytotoxic activity of calcein acetoxymethyl ester (calcein/AM) on primary cultures of human haematological and solid tumours. Eur. J. Cancer. 32A:1996;883.
27. Keeney M., Chin-Yee I., Weir K., Popma J., Nayar R., Sutherland D.R. Single platform flow cytometric absolute CD34+ cell counts based on the ISHAGE guidelines. Cytometry (Comm. Clin. Cytometry). 34:1998;61.
28. King M.A., Radicchi-Mastroianni M.A. Use of four-colour flow cytometry to evaluate conjugate formation between human peripheral blood mononuclear cells and tumour target cells. Cytometry. 24:1996;368.
29. King M.A., Wu Q.-X., Donovan G.R., Baldo B.A. Flow cytometric analysis of cell killing by the jumper ant venom peptide pilosulin I. Cytometry. 32:1998;268.
30. Krishan A. Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining. J. Cell Biol. 66:1975;188.
31. Levesque A., Paquet A., Page M. Measurement of tumor necrosis factor activity by flow cytometry. Cytometry. 20:1995;181.
32. Liminga G., Nygren P., Dhar S., Nilsson K., Larsson R. Cytotoxic effect of calcein acetoxymethyl ester on human tumor cell lines: drug delivery by intracellular trapping. Anti-Cancer Drugs. 6:1995;578.
33. Loken M.R., Stall A.M. Flow cytometry as an analytical and preparative tool in immunology. J. Immunol. Methods. 50:1982;85.
34. Massaro E.J., Elstein K.H., Zucker R.M., Bair K.W. Limitations of the fluorescent probe viability assay. Mol. Toxicol. 2:1989;271.
35. McGinnes K., Chapman G., Marks R., Penny R. A fluorescence NK assay using flow cytometry. J. Immunol. Methods. 86:1986;7.
36. O'Brien M., Bolton W.E. Comparison of cell viability probes compatible with fixation and permeabilization for combined surface and intracellular staining in flow cytometry. Cytometry. 19:1995;243.
37. Papa S., Vitale M., Mariani A.R., Roda P., Facchini A., Manzoli F.A. Natural killer function in flow cytometry. I. Evaluation of NK lytic activity on K562 line. J. Immunol. Methods. 107:1988;73.
38. Papadopoulos N.G., Dedoussis G.V.Z., Spanakos G., Gritzapis A.D., Baxevanis C.N., Papamichail M. An improved fluorescence assay for the determination of lymphocyte-mediated cyotoxicity using flow cytometry. J. Immunol. Methods. 177:1994;101.
39. Radcliff G., Waite R., LeFevre J., Poulik M.D., Callewaert D.M. Quantification of effector/target conjugation involving natural killer (NK) or lymphokine activated killer (LAK) cells by two-color flow cytometry. J. Immunol. Methods. 139:1991;281.
40. Radosevic K., Garritsen H.S.P., Van Graft M., De Grooth B.G., Greve J. A simple and sensitive flow cytometric assay for the determination of the cytotoxic activity of human natural killer cells. J. Immunol. Methods. 135:1990;81.
41. Riedy M.C., Muirhead K.A., Jensen C.P., Stewart C.C. Use of a photolabeling technique to identify nonviable cells in fixed homologous or heterologous cell populations. Cytometry. 12:1991;133.
42. Roper P.R., Drewinko B. Comparison of in vitro methods to determine drug-induced cell lethality. Cancer Res. 36:1976;2182.
43. Ross D.D., Joneckis C.C., Ordonez J.V., Sisk A.M., Wu R.K., Hamburger A.W., Nora R.E. Estimation of cell survival by flow cytometric quantification of fluorescein diacetate/propidium iodide viable cell number. Cancer Res. 49:1989;3776.
44. Roth B.L., Poot M., Yue S.T., Millard P.J. Bacterial viability and antibiotic susceptibility testing with SYTOX green nucleic acid stain. Appl. Environ. Microbiol. 63:1997;2421.
45. Rotman B., Papermaster B.W. Membrane properties of living mammalian cells as studied by enzymatic hydrolysis of fluorogenic esters. Proc. Natl. Acad. Sci. 55:1966;134.
46. Sasaki D.T., Dumas S.E., Engelman E.G. Discrimination of viable and non-viable cells using propidium iodide in two color immunofluorescence. Cytometry. 8:1987;413.
47. Schmid I., Krall W.J., Uittenbogaart C.H., Braun J., Giorgi J.V. Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry. Cytometry. 13:1992;204.
48. Schmid I., Ferbas J., Uittenbogaart C.H., Giorgi J.V. Flow cytometric analysis of live cell proliferation and phenotype in populations with low viability. Cytometry. 35:1999;64.
49. Sellers J.R., Cook S., Goldmacher V.S. A cytotoxicity assay utilizing a fluorescent dye that determines accurate surviving fractions of cells. J. Immunol. Methods. 172:1994;255.
50. Shapiro H.M. Parameters and probes. Practical Flow Cytometry. 3rd Edition :1995;229 Wiley, New York.
51. Shi T.-X., Tong M.J., Bohman R. The application of flow cytometry in the study of natural killer cell cytotoxicity. Clin. Immunol. Immunopathol. 45:1987;356.
52. Slezak S.E., Horan P.K. Cell-mediated cytotoxicity. A highly sensitive and informative flow cytometric assay. J. Immunol. Methods. 117:1989;205.
53. Taga K., Yamauchi A., Kabashima K., Bloom E.T., Muller J., Tosato G. Target-induced death by apoptosis in human lymphokine-activated natural killer cells. Blood. 87:1996;2411.
54. Vermes, I., Haanen, C., Reutelingsperger, C., 2000. Flow cytometry of apoptotic cell death. J. Immunol. Methods (in press).
55. Vitale M., Neri L.M., Comani S., Falcieri E., Rizzoli R., Rana R., Papa S. Natural killer function in flow cytometry. II. Evaluation of NK lytic activity by means of target cell morphological changes detected by right angle light scatter. J. Immunol. Methods. 121:1989;115.
56. Wallen C.A., Higashikubo R., Roti Roti J.L. Comparison of the cell kill measured by the Hoechst-propidium iodide flow cytometric assay and the colony formation assay. Cell Tissue Kinet. 16:1983;357.
57. Weisenthal L.M., Dill P.L., Kurnick N.B., Lippman M.E. Comparison of dye exclusion assays with a clonogenic assay in the determination of drug-induced cytotoxicity. Cancer Res. 43:1983;258.
58. Weisenthal L.M., Lippman M.E. Clonogenic and nonclonogenic in vitro chemosensitivity assays. Cancer Treat. Rep. 69:1985;1985.
59. Weston K.M., Alsalami M., Raison R.L. Cell membrane changes induced by the cytolytic peptide, melittin, are detectable by 90° laser scatter. Cytometry. 15:1994;141.
60. Weston S.A., Parish C.R. New fluorescent dyes for lymphocyte migration studies. Analysis by flow cytometry and fluorescence microscopy. J. Immunol. Methods. 133:1990;87.
61. Wing M.G., Montgomery A.M.P., Songsivilai S., Watson J.V. An improved method for the detection of cell surface antigens in samples of low viability using flow cytometry. J. Immunol. Methods. 126:1990;21.
62. Yuhas J.M., Toya R.E., Pazmino N.H. Neuraminidase and cell viability: failure to detect cytotoxic effects with dye-exclusion techniques. J. Natl. Cancer Inst. 53:1974;465.
63. Zamai L., Bareggi R., Santavenere E., Vitale M. Subtraction of autofluorescent dead cells from the lymphocyte flow cytometric binding assay. Cytometry. 14:1993;951.