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Imaged the fast T-cell movements in lymph nodes in a living animal in the absence of antigen, demonstrating migration into the node.
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Used a PI3K reporter to show that long-term signalling and the IS are required for full T-cell activation.
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Repeatedly blocked signalling with a kinase inhibitor, then released the block by washing. Found that the T cell added up the amount of signal accumulated over the unblocked periods.
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Demonstrated that activation by a low concentration of antigen gave a higher than expected level of MHC and TCR recruitment to the IS. Found that non-stimulatory MHCp complexes (but not antagonist MHCp) were also recruited. Also demonstrated the importance of co-stimulation in IS formation.
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