Direct Chemical Evidence for Charge Transfer between Photoexcited 2-Aminopurine and Guanine in Duplex DNA

Journal of the American Chemical Society

Volumn 126, Issue 5, 2004, Pages 1316-1317

  O'Neill, Melanie A ^a   Dohno, Chikara ^a   Barton, Jacqueline K ^a  

^a California Institute of Technology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2 AMINOPURINE; DOUBLE STRANDED DNA; GUANINE;

ARTICLE; DNA DAMAGE; DNA SEQUENCE; DNA STRUCTURE; ELECTRON TRANSPORT; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IRRADIATION; OXIDATION; PHOTOCHEMISTRY; PHOTODEGRADATION; SURFACE CHARGE;

2-AMINOPURINE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DNA; GUANINE; PHOTOCHEMISTRY;

EID: 1042265093     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja037802p     Document Type: Article

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28. CPG nucleoside (Supporting Information).
29. CPG was prepared as described elsewhere,15 twice purified by HPLC, and analyzed by ESI mass spectrometry. Duplexes (5 μM in 100 mM sodium phosphate, pH 7) were irradiated with a mercury-xenon lamp (3 mW) ntW) at 325 nm (320 nm LP filter) and digested by 37 °C incubation with phosphodiesterase, P1 endonuclease, and alkaline phosphatase for 2 h. The nucleosides were separated by reverse phase HPLC and identified with authentic standards (Supporting Information).
30. HPG and dG (Scheme).15
31. CPG.
32. CPG using cyanobenzophenone-modified uridine (CNBPU) did not lead to significant loss.15
33. -1 at 325 nm for Ap in DNA.
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35. 9b
36. cpGG doublet, see sequences Scheme 1.
37. CPG is comparable to that in ApAG, indicating that the forward CT does occur.