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Volumn 122, Issue 15, 2000, Pages 3585-3594

DNA-protein cross-linking from oxidation of guanine via the flash-quench technique

Author keywords

[No Author keywords available]

Indexed keywords

1,10 PHENANTHROLINE; DNA; GUANINE; HISTONE; PHENAZINE DERIVATIVE; RUTHENIUM COMPLEX;

EID: 0034685477     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja993502p     Document Type: Article
Times cited : (52)

References (99)
  • 64
    • 0343410153 scopus 로고    scopus 로고
    • note
    • Because we observed only the neutral deprotonated radical form in earlier flash-quench experiments (ref 16), this is the form depicted in the scheme. However, the guanine cation radical initially produced upon 1-electron oxidation can also be hydrated to form the 8-hydroxy-7,8-dihydroguanyl radical (see refs 8d and 13).
  • 69
    • 0343846084 scopus 로고    scopus 로고
    • note
    • It is not surprising that a full restoration of mobility is not observed, since the protease will likely leave a peptide fragment on the DNA, owing to steric restraints.
  • 70
    • 0000860788 scopus 로고    scopus 로고
    • 2+ is very small, and thus observed only at much longer irradiation times than are used in the cross-linking experiments.
    • (1986) J. Am. Chem. Soc. , vol.108 , pp. 7414
    • Mei, H.-Y.1    Barton, J.K.2
  • 72
    • 0000860788 scopus 로고    scopus 로고
    • Ph.D. Thesis, California Institute of Technology
    • 2+ is very small, and thus observed only at much longer irradiation times than are used in the cross-linking experiments.
    • (1996)
    • Jenkins, Y.C.1
  • 78
    • 0342540563 scopus 로고    scopus 로고
    • note
    • Indeed the quenching here is more efficient than for oligonucleotide duplexes in the absence of histone. This can be attributed to the fact that the quenching process, which requires diffusion of the quencher to the metallointercalator along the DNA, is more efficient for longer strands of DNA.
  • 79
    • 0342540564 scopus 로고    scopus 로고
    • note
    • a of 9.5 (See ref 2b).
  • 83
    • 0343846080 scopus 로고    scopus 로고
    • note
    • 1+ intermediate.
  • 84
    • 0342974840 scopus 로고    scopus 로고
    • note
    • -1 has been estimated (see ref 16).
  • 88
    • 0342540561 scopus 로고    scopus 로고
    • note
    • (d) Transfer of the electron hole from guanine to protein may produce reactive intermediates on the protein that could participate in cross-linking reactions. We are investigating this possibility.
  • 91
    • 0343846079 scopus 로고    scopus 로고
    • note
    • Two of the competing pathways for decay of the guanine cation radical are deprotonation and hydration. Formation of 8-oxo-G. which proceeds via hydration of the cation radical, is greatly enhanced when the guanine is in DNA rather than in solution as a free nucleoside. Hydration apparently competes more favorably with deprotonation once the guanine base is part of the DNA polymer. See refs 8d, 13 and 37.
  • 92
    • 0343410147 scopus 로고    scopus 로고
    • note
    • 2+ as quencher does not produce detectable amounts of 8-oxo-G. Perhaps 8-oxo-G is oxidized further with these quenchers to produce an 8-oxo-G radical, which could react with protein by a mechanism similar to that reported by Hickerson et al. (ref 24). Alternatively, the longer lifetime of the guanine radical in these cases may be responsible for the different distribution of guanine oxidation products.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.