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Indeed the quenching here is more efficient than for oligonucleotide duplexes in the absence of histone. This can be attributed to the fact that the quenching process, which requires diffusion of the quencher to the metallointercalator along the DNA, is more efficient for longer strands of DNA.
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a of 9.5 (See ref 2b).
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Two of the competing pathways for decay of the guanine cation radical are deprotonation and hydration. Formation of 8-oxo-G. which proceeds via hydration of the cation radical, is greatly enhanced when the guanine is in DNA rather than in solution as a free nucleoside. Hydration apparently competes more favorably with deprotonation once the guanine base is part of the DNA polymer. See refs 8d, 13 and 37.
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