Characterisation of human dipeptidyl peptidase IV expressed in Pichia pastoris. A structural and mechanistic comparison between the recombinant human and the purified porcine enzyme

Biological Chemistry

Volumn 384, Issue 12, 2003, Pages 1553-1563

  Bär, Joachim ^a   Weber, Anja ^a   Hoffmann, Torsten ^a   Stork, Jörg ^a   Wermann, Michael ^a   Wagner, Leona ^a   Aust, Susanne ^a   Gerhartz, Bernd ^a,b   Demuth, Hans Ulrich ^a  

^a PROBIODRUG AG   (Germany)

^b NOVARTIS PHARMA AG   (Switzerland)

Author keywords

Adenosine deaminase; Dipeptidyl peptidase IV; DNA sequence

Indexed keywords

ADENOSINE DEAMINASE; DIPEPTIDYL PEPTIDASE IV; ENZYME INHIBITOR; GLUCAGON LIKE PEPTIDE 1 [7-36] AMIDE; RECOMBINANT ENZYME;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; ENZYME ASSAY; ENZYME BINDING; ENZYME GLYCOSYLATION; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME STRUCTURE; ENZYME SUBSTRATE; FERMENTATION TECHNIQUE; HUMAN; KIDNEY; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PROTEIN INTERACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY; SPECIES DIFFERENCE; SWINE;

ADENOSINE DEAMINASE; AMINO ACID SEQUENCE; ANIMALS; ANTIGENS, CD26; BASE SEQUENCE; CATALYTIC DOMAIN; CLONING, MOLECULAR; DIPEPTIDES; ENZYME INHIBITORS; FLUORESCENT DYES; GLUCAGON; GLUCAGON-LIKE PEPTIDE 1; GLUCAGON-LIKE PEPTIDES; GLYCOPROTEINS; HUMANS; ISOELECTRIC POINT; KIDNEY; KINETICS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PEPTIDE FRAGMENTS; PEPTIDES; PICHIA; PROTEIN BINDING; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY; SWINE;

ANIMALIA; PICHIA; PICHIA PASTORIS; SUIDAE; SUS SCROFA;

EID: 0347989249     PISSN: 14316730     EISSN: None     Source Type: Journal    
DOI: 10.1515/BC.2003.172     Document Type: Article

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