Use of human reconstructed epidermis to analyze the regulation of β-defensin hBD-1, hBD-2, and hBD-3 expression in response to LPS

Cell Biology and Toxicology

Volumn 19, Issue 5, 2003, Pages 313-324

  Chadebech, P ^a   Goidin, D ^a   Jacquet, C ^a   Viac, J ^a   Schmitt, Daniel ^a   Staquet, M J ^a  

^a EDOUARD HERRIOT HOSPITAL   (France)

Author keywords

Antimicrobial peptide; Keratinocytes; Skin

Indexed keywords

BETA DEFENSIN 1; BETA DEFENSIN 2; BETA DEFENSIN 3; CALCIUM; LIPOPOLYSACCHARIDE; MESSENGER RNA;

ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CELL GROWTH; CELL PROLIFERATION; EPIDERMIS; GENE EXPRESSION REGULATION; GENETIC CODE; HUMAN; HUMAN CELL; IN VITRO STUDY; KERATINOCYTE; NORMAL HUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; WESTERN BLOTTING;

BETA-DEFENSINS; BLOTTING, WESTERN; CALCIUM; CELL DIFFERENTIATION; CELL DIVISION; CELLS, CULTURED; EPIDERMIS; GENE EXPRESSION REGULATION; HUMANS; IMMUNOHISTOCHEMISTRY; KERATINOCYTES; LIPOPOLYSACCHARIDES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; TIME FACTORS; UP-REGULATION;

BACTERIA (MICROORGANISMS);

EID: 0347478183     PISSN: 07422091     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:CBTO.0000004975.36521.c8     Document Type: Article

References (41)

1. Abiko Y, Nishimura M, Kusano K, et al. Upregulated expression of human β defensin-1 and -3 mRNA during differentiation of keratinocyte immortalized cell lines, HaCaT and PHK16-Ob. J Dermatol Sci. 2003;31:225-8.
2. Ali S, Falconer A, Ikram M, Bisset CE, Cerio R, Quinn AG. Expression of the peptide antibiotics human β defensin-1 and human β defensin-2 in normal human skin. J Invest Dermatol. 2001;117:106-11.
3. Bals R, Wang X, Wu Z, et al. Human beta-defensin 2 is a salt-sensitive peptide antibiotic expressed in human lung. J Clin Invest. 1998;102:874-80.
4. Berthod F, Damour O. In vitro reconstructed skin models for wound coverage in deep burns. Br J Dermatol. 1997;136:809-16.
5. Boman HG. Peptide antibiotics and their role in innate immunity. Annu Rev Immunol. 1995;13:61-92.
6. Dunsche A, Acil Y, Dommisch H, Siebert R, Schroder JM, Jepsen S. The novel human beta-defensin-3 is widely expressed in oral tissues. Eur J Oral Sci. 2002;110:121-4.
7. Frohm M, Agerberth B, Ahangari G, et al. The expression of the gene coding for the antibacterial peptide LL-37 is induced in human keratinocytes during inflammatory disorders. J Biol Chem. 1997;272:15258-63.
8. Frye M, Bargon J, Lembcke B, Wagner TO, Gropp R. Differential expression of human alpha- and beta-defensins mRNA in gastrointestinal epithelia. Eur J Clin Invest. 2000;30:695-701.
9. Fulton C, Anderson GM, Zasloff M, Bull R, Quinn AG. Expression of natural peptide antibiotics in human skin. Lancet. 1997;350:1750-1.
10. Ganz T, Lehrer RI. Defensins. Curr Opin Immunol. 1994;6:584-9.
11. Garcia J-RC, Jaumann F, Schulz S, et al. Identification of a novel, multifunctional β-defensin (human β-defensin 3) with specific antimicrobial activity. Its interaction with plasma membranes of Xenopus oocytes and the induction of macrophage chemoattraction. Cell Tissue Res. 2001a;306:257-64.
12. Garcia J-RC, Krause A, Schulz S, et al. Human β-defensin 4: a novel inducible peptide with a specific salt-sensitive spectrum of antimicrobial activity. FASEB J. 2001 b;15:1819-21.
13. Gottwald E, Mueller O, Polten A. Semiquantitative reverse transcription-polymerase chain reaction with the Agilent 2100 Bioanalyzer. Electrophoresis. 2001;22:4016-22.
14. Hancock REW, Diamond G. The role of cationic antimicrobial peptides in innate host defenses. Trends Microbiol. 2000;8:402-10.
15. Harder J, Bartels J, Christophers E, Schroder JM. A peptide antibiotic from human skin. Nature. 1997;387:861.
16. Harder J, Meyer-Hoffert U, Teran LM, et al. Mucoid Pseudomonas aerughzosa, TNF-alpha, and IL-1 beta, but not IL-6, induce human beta-defensin-2 in respiratory epithelia. Am J Respir Cell Mol Biol. 2000;22:714-21.
17. Harder J, Bartels J, Christophers E, Schroder JM. Isolation and characterization of human beta-defensin-3, a novel human inducible peptide antibiotic. J Biol Chem. 2001;276:5707-13.
18. Krisanaprakornkit S, Kimball JR, Weinberg A, Darveau RP, Bainbridge BW, Dale BA. Inducible expression of human beta-defensin 2 by Fusobacterium nucleatum in oral epithelial cells: multiple signaling pathways and role of commensal bacteria in innate immunity and the epithelial barrier. Infect Immun. 2000;68:2907-15.
19. Lehrer RI, Ganz T. Cathelicidins: a family of endogenous antimicrobial peptides. Curr Opin Hematol. 2002a;9:18-22.
20. Lehrer RI, Ganz T. Defensins of vertebrate animals. Curr Opin Immunol. 2002b;14:96-102.
21. Liu AY, Destoumieux D, Wong AV. et al. Human β-defensin-2 production in keratinocytes is regulated by interleukin-1, bacteria, and the state of differentiation. J Invest Dermatol. 2002;118:275-81.
22. Midorikawa K, Ouhara K, Komatsuzawa H, et al. Staphylococcus aureus susceptibility to innate antimicrobial peptides, β-defensins and CAP18, expressed by human keratinocytes. Infect Immun. 2003;71:3730-9.
23. Mueller O, Hahnenberger K, Dittmann M, et al. A microfluidic system for high-speed reproducible DNA sizing and quantitation. Electrophoresis. 2000;21:128-34.
24. Nizet V, Ohtake T, Lauth X, et al. Innate antimicrobial peptide protects the skin from invasive bacterial infection. Nature. 2001;22:454-7.
25. O'Neil DA, Cole SP, Martin-Porter E, et al. Regulation of human beta-defensins by gastric epithelial cells in response to infection with Helicobacter pylori or stimulation with interleukin-1. Infect Immun. 2000;68:5412-5.
26. O'Neil DA, Porter EM, Elewaut D, et al. Expression and regulation of the human beta-defensins hBD-1 and hBD-2 in intestinal epithelium. J Immunol. 1999;163:6718-24.
27. Oren A, Ganz T, Liu L, Meerlo T. In human epidermis, β-defensin 2 is packaged in lamellar bodies. Exp Mol Pathol. 2003;74:180-2.
28. Ortega MR, Ganz T, Milner SM. Human beta defensin is absent in burn blister fluid. Burns. 2000;26:724-6.
29. Pernet I, Reymermier C, Guezennec A, et al. Calcium triggers β-defensin (hBD-2 and hBD-3) and chemokine macrophage inflammatory protein 3α (MIP-3α(/CCL20) expression in monolayers of activated human keratinocytes. Exp Dermatol. In press.
30. Raj PA, Dentino AR. Current status of defensins and their role in innate and adaptive immunity. FEMS Microbiol Lett. 2002;206:9-18.
31. Regnier M, Asselineau D, Lenoir MC. Human epidermis reconstructed on dermal substrates in vitro: an alternative to animals 3:70-85.
32. Regnier M, Staquet MJ, Schmitt D, Schmidt R. Integration of Langerhans cells into a pigmented reconstructed human epidermis. J Invest Dermatol. 1997;109:510-2.
33. Rheinwald JG, Green H. Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell. 1975;6:331-43.
34. Schroder JM, Harder J. Human beta-defensin-2. Int J Biochem Cell Biol. 1999;31:645-51.
35. Seo SJ, Ahn SW, Hong CK, Ro BI. Expressions of β-defensins in human keratinocyte cell lines. J Dermatol Sci. 2001;27:183-91.
36. Singh PK, Peng Jia H, Wiles K, et al. Production of beta-defensins by human airway epithelia. Proc Natl Acad Sci USA. 1998;95:14961-6.
37. Stolzenberg ED, Anderson GM, Ackermann MR, Whitlock RH, Zasloff M. Epithelial antibiotic induced in states of disease. Proc Natl Acad Sci USA. 1997;94:8686-90.
38. van Wetering S, Sterk PJ, Rabe KF, Hiemstra PS. Defensins: key players or bystanders in infection, injury, and repair in the lung? J Allergy Clin Immunol. 1999;104:1131-8.
39. Yang D, Chertov O, Bykovskaia SN, et al. β-defensins: linking innate and adaptive immunity through dendritic and T cell CCR6. Science. 1999;286:525-8.
40. Yang D, Biragyn A, Kwak LW, Oppenheim JJ. Mammalian defensins in immunity: more than just microbicidal. Trends Immunol. 2002;23:291-5.
41. Zhao C, Wang I, Lehrer RI. Widespread expression of beta-defensin hBD-1 in human secretory glands and epithelial cells. FEBS Lett. 1996;396:319-22.