Purified complexes of HIV-1 envelope glycoproteins with CD4 and CCR5(CXCR4): Production, characterization and immunogenicity

Vaccine

Volumn 21, Issue 27-30, 2003, Pages 4275-4284

  Xiao, Xiaodong ^a   Phogat, Sanjay ^a   Shu, Yuuei ^a   Phogat, Adhuna ^a   Chow, Yen Hung ^a   Wei, Olivia L ^b   Goldstein, Harris ^c   Broder, Christopher C ^b   Dimitrov, Dimiter S ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

^b UNIFORMED SERVICES UNIVERSITY OF THE HEALTH SCIENCES   (United States)

^c ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

Author keywords

AIDS; CCR5; CD4; CXCR4; gp120; gp140; gp41; HIV

Indexed keywords

ANTIGEN; CD4 ANTIGEN; CHEMOKINE RECEPTOR CCR5; EPITOPE; GLYCOPROTEIN; GLYCOPROTEIN GP 140; NEUTRALIZING ANTIBODY; RECEPTOR; VIRUS VACCINE;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; COMPARATIVE STUDY; CONTROLLED STUDY; EVALUATION; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; HYPOTHESIS; IMMUNOGENICITY; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; VIRUS ENVELOPE; VIRUS ISOLATION;

EID: 0347448154     PISSN: 0264410X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0264-410X(03)00494-8     Document Type: Article

References (29)

1. Sattentau Q.J., Moore J.P. Conformational changes induced in the human immunodeficiency virus envelope glycoproteins by soluble CD4 binding. J. Exp. Med. 174:1991;407-415.
2. Jones P.L., Korte T., Blumenthal R. Conformational changes in cell surface HIV-1 envelope glycoproteins are triggered by cooperation between cell surface CD4 and co-receptors. J. Biol. Chem. 273(1):1998;404-409.
3. Dimitrov D.S. Cell biology of virus entry. Cell. 101(7):2000;697-702.
4. Celada F., Cambiaggi C., Maccari J.et al. Antibody raised against soluble CD4-rgp120 complex recognizes the CD4 moiety and blocks membrane fusion without inhibiting CD4-gp120 binding. J. Exp. Med. 172:1990;1143-1150.
5. Gershoni J.M., Denisova G., Raviv D., Smorodinsky N.I., Buyaner D. HIV binding to its receptor creates specific epitopes for the CD4/gp120 complex. FASEB J. 7(12):1993;1185-1187.
6. Kang C.Y., Hariharan K., Nara P.L., Sodroski J., Moore J.P. Immunization with a soluble CD4-gp120 complex preferentially induces neutralizing anti-human immunodeficiency virus type 1 antibodies directed to conformation-dependent epitopes of gp120. J. Virol. 68(9):1994;5854-5862.
7. DeVico A.L., Rahman R., Welch J.et al. Monoclonal antibodies raised against covalently crosslinked complexes of human immunodeficiency virus type 1 gp120 and CD4 receptor identify a novel complex-dependent epitope on gp 120. Virology. 211(2):1995;583-588.
8. Devico A., Silver A., Thronton A.M., Sarngadharan M.G., Pal R. Covalently crosslinked complexes of human immunodeficiency virus type 1 (HIV-1) gp120 and CD4 receptor elicit a neutralizing immune response that includes antibodies selective for primary virus isolates. Virology. 218(1):1996;258-263.
9. Fouts T., Godfrey K., Bobb K.et al. Crosslinked HIV-1 envelope-CD4 receptor complexes elicit broadly cross-reactive neutralizing antibodies in rhesus macaques. Proc. Natl. Acad. Sci. U.S.A. 99(18):2002;11842-11847.
10. Dimitrov D.S. Fusin - a place for HIV-1 and T4 cells to meet. Identifying the coreceptor mediating HIV-1 entry raises new hopes in the treatment of AIDS. Nat. Med. 2:1996;640-641.
11. Dimitrov AS, Xiao X, Dimitrov DS, Blumenthal R. Early intermediates in HIV-1 envelope glycoprotein-mediated fusion triggered by CD4 and coreceptor complexes. J. Biol. Chem. 2001;276(32):30335-41.
12. Moulard M., Phogat S.K., Shu Y.et al. Broadly cross-reactive HIV-1-neutralizing human monoclonal Fab selected for binding to gp120-CD4-CCR5 complexes. Proc. Natl. Acad. Sci. U.S.A. 99(10):2002;6913-6918.
13. Babcock G.J., Mirzabekov T., Wojtowicz W., Sodroski J. Ligand-binding characteristics of CXCR4 incorporated into paramagnetic proteoliposomes. J. Biol. Chem. 276:2001;38433-38440.
14. Mirzabekov T., Bannert N., Farzan M.et al. Enhanced expression, native purification, and characterization of CCR5, a principal HIV-1 coreceptor. J. Biol. Chem. 274(40):1999;28745-28750.
15. Lapham C.K., Ouyang J., Chandrasekhar B., Nguyen N.Y., Dimitrov D.S., Golding H. Evidence for cell-surface association between fusin and the CD4-gp120 complex in human cell lines. Science. 274:1996;602-605.
16. Xiao X., Norwood D., Feng Y.R.et al. Inefficient formation of a complex among CXCR4, CD4 and gp120 in U937 clones resistant to X4 gp120-gp41-mediated fusion. Exp. Mol. Pathol. 68(3):2000;139-146.
17. Xiao X., Wu L., Stantchev T.S.et al. Constitutive cell surface association between CD4 and CCR5. Proc. Natl. Acad. Sci. U.S.A. 96(13):1999;7496-7501.
18. Nussbaum O., Broder C.C., Berger E.A. Fusogenic mechanisms of enveloped-virus glycoproteins analyzed by a novel recombinant vaccinia virus-based assay quantitating cell fusion-dependent reporter gene activation. J. Virol. 68(9):1994;5411-5422.
19. Feng Y., Broder C.C., Kennedy P.E., Berger E.A. HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor. Science. 272:1996;872-877.
20. Earl P.L., Broder C.C., Long D.et al. Native oligomeric human immunodeficiency virus type 1 envelope glycoprotein elicits diverse monoclonal antibody reactivities. J. Virol. 68(5):1994;3015-3026.
21. Earl P.L., Sugiura W., Montefiori D.C.et al. Immunogenicity and protective efficacy of oligomeric human immunodeficiency virus type 1 gp140. J. Virol. 75(2):2001;645-653.
22. Browning J., Horner J.W., Pettoello-Mantovani M.et al. Mice transgenic for human CD4 and CCR5 are susceptible to HIV infection. Proc. Natl. Acad. Sci. U.S.A. 94(26):1997;14637-14641.
23. Connor R.I., Chen B.K., Choe S., Landau N.R. Vpr is required for efficient replication of human immunodeficiency virus type-1 in mononuclear phagocytes. Virology. 206(2):1995;935-944.
24. Collman R., Balliet J.W., Gregory S.A.et al. An infectious molecular clone of an unusual macrophage-tropic and highly cytopathic strain of human immunodeficiency virus type 1. J. Virol. 66(12):1992;7517-7521.
25. O'Brien W.A., Koyanag Y., Namzie A.et al. HIV-1 tropism for mononuclear phagocytes can be determined by regions of gp120 outside the CD4-binding domain. Nature. 348:1990;69-73.
26. Adachi A., Gendelman H.E., Koenig S.et al. Production of acquired immunodeficiency syndrome-associated retrovirus in human and non-human cells transfected with an infectious molecular clone. J. Virol. 59:1986;284-291.
27. Letvin N.L., Barouch D.H., Montefiori D.C. Prospects for vaccine protection against HIV-1 infection and AIDS. Annu. Rev. Immunol. 20:2002;73-99.
28. Kwong P.D., Doyle M.L., Casper D.J.et al. HIV-1 evades antibody-mediated neutralization through conformational masking of receptor-binding sites. Nature. 420(6916):2002;678-682.
29. Chow Y.H., Wei O.L., Phogat S.et al. Conserved structures exposed in HIV-1 envelope glycoproteins stabilized by flexible linkers as potent entry inhibitors and potential immunogens. Biochemistry. 41(22):2002;7176-7182.