Evidence for cell-surface association between fusin and the CD4-gp120 complex in human cell lines

Science

Volumn 274, Issue 5287, 1996, Pages 602-605

  Lapham, Cheryl K ^a   Ouyang, Jun ^b   Chandrasekhar, Bhaskar ^a   Nguyen, Nga Y ^a   Dimitrov, Dimiter S ^b   Golding, Hana ^a  

^a CENTER FOR BIOLOGICS EVALUATION AND RESEARCH   (United States)

^b NATIONAL CANCER INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CD4 ANTIGEN; GLYCOPROTEIN GP 120; PHORBOL 13 ACETATE 12 MYRISTATE;

ARTICLE; CONTROLLED STUDY; DOWN REGULATION; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; MEMBRANE FUSION; NONHUMAN; PRIORITY JOURNAL; T LYMPHOCYTE; VIRUS CELL INTERACTION; VIRUS ENVELOPE;

3T3 CELLS; AMINO ACID SEQUENCE; ANIMALS; ANTIGENS, CD4; CELL LINE; CELL MEMBRANE; GIANT CELLS; HIV ENVELOPE PROTEIN GP120; HUMANS; IMMUNOBLOTTING; MEMBRANE FUSION; MEMBRANE PROTEINS; MICE; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PRECIPITIN TESTS; RECEPTORS, CXCR4; RECEPTORS, HIV; T-LYMPHOCYTES; TETRADECANOYLPHORBOL ACETATE; VACCINIA VIRUS;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; ORYCTOLAGUS CUNICULUS; VACCINIA;

EID: 0029805881     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.274.5287.602     Document Type: Article

References (22)

1. P. Ashorn, E. Berget, B. Moss, J. Virol. 64, 2149 (1990); B. Asjo et al., Lancet ii. 660 (1986); C. Cheng-Mayer et al., J, Virol. 64, 4390 (1990); B. Chesebro et al., ibid. 65, 5782 (1991); C. C. Broder, D. S. Dimitrov, R. Blumenthal, E. A. Berger, Virology 193, 483 (1993); C. C. Broder and E. A. Berger, Proc. Natl. Acad. Sci. U.S.A. 92, 9004 (1995).
2. P. Ashorn, E. Berget, B. Moss, J. Virol. 64, 2149 (1990); B. Asjo et al., Lancet ii. 660 (1986); C. Cheng-Mayer et al., J, Virol. 64, 4390 (1990); B. Chesebro et al., ibid. 65, 5782 (1991); C. C. Broder, D. S. Dimitrov, R. Blumenthal, E. A. Berger, Virology 193, 483 (1993); C. C. Broder and E. A. Berger, Proc. Natl. Acad. Sci. U.S.A. 92, 9004 (1995).
3. P. Ashorn, E. Berget, B. Moss, J. Virol. 64, 2149 (1990); B. Asjo et al., Lancet ii. 660 (1986); C. Cheng-Mayer et al., J, Virol. 64, 4390 (1990); B. Chesebro et al., ibid. 65, 5782 (1991); C. C. Broder, D. S. Dimitrov, R. Blumenthal, E. A. Berger, Virology 193, 483 (1993); C. C. Broder and E. A. Berger, Proc. Natl. Acad. Sci. U.S.A. 92, 9004 (1995).
4. P. Ashorn, E. Berget, B. Moss, J. Virol. 64, 2149 (1990); B. Asjo et al., Lancet ii. 660 (1986); C. Cheng-Mayer et al., J, Virol. 64, 4390 (1990); B. Chesebro et al., ibid. 65, 5782 (1991); C. C. Broder, D. S. Dimitrov, R. Blumenthal, E. A. Berger, Virology 193, 483 (1993); C. C. Broder and E. A. Berger, Proc. Natl. Acad. Sci. U.S.A. 92, 9004 (1995).
5. P. Ashorn, E. Berget, B. Moss, J. Virol. 64, 2149 (1990); B. Asjo et al., Lancet ii. 660 (1986); C. Cheng-Mayer et al., J, Virol. 64, 4390 (1990); B. Chesebro et al., ibid. 65, 5782 (1991); C. C. Broder, D. S. Dimitrov, R. Blumenthal, E. A. Berger, Virology 193, 483 (1993); C. C. Broder and E. A. Berger, Proc. Natl. Acad. Sci. U.S.A. 92, 9004 (1995).
6. P. Ashorn, E. Berget, B. Moss, J. Virol. 64, 2149 (1990); B. Asjo et al., Lancet ii. 660 (1986); C. Cheng-Mayer et al., J, Virol. 64, 4390 (1990); B. Chesebro et al., ibid. 65, 5782 (1991); C. C. Broder, D. S. Dimitrov, R. Blumenthal, E. A. Berger, Virology 193, 483 (1993); C. C. Broder and E. A. Berger, Proc. Natl. Acad. Sci. U.S.A. 92, 9004 (1995).
7. Y. Feng, C. C. Broder, P. E. Kennedy, E. A. Berger, Science 272, 872 (1996).
8. G. Alkhatib et al., ibid., p. 1955; H. Choe et al., Cell 85, 1135 (1996); B. J. Doranz et al., ibid., p. 1149; H Deng et al., Nature 381, 661 (1996) ; T, Dragic et al. ibid., p. 667; J. F, Berson et al., J. Virol. 70, 6288 (1996).
9. G. Alkhatib et al., ibid., p. 1955; H. Choe et al., Cell 85, 1135 (1996); B. J. Doranz et al., ibid., p. 1149; H Deng et al., Nature 381, 661 (1996) ; T, Dragic et al. ibid., p. 667; J. F, Berson et al., J. Virol. 70, 6288 (1996).
10. G. Alkhatib et al., ibid., p. 1955; H. Choe et al., Cell 85, 1135 (1996); B. J. Doranz et al., ibid., p. 1149; H Deng et al., Nature 381, 661 (1996) ; T, Dragic et al. ibid., p. 667; J. F, Berson et al., J. Virol. 70, 6288 (1996).
11. G. Alkhatib et al., ibid., p. 1955; H. Choe et al., Cell 85, 1135 (1996); B. J. Doranz et al., ibid., p. 1149; H Deng et al., Nature 381, 661 (1996) ; T, Dragic et al. ibid., p. 667; J. F, Berson et al., J. Virol. 70, 6288 (1996).
12. G. Alkhatib et al., ibid., p. 1955; H. Choe et al., Cell 85, 1135 (1996); B. J. Doranz et al., ibid., p. 1149; H Deng et al., Nature 381, 661 (1996) ; T, Dragic et al. ibid., p. 667; J. F, Berson et al., J. Virol. 70, 6288 (1996).
13. G. Alkhatib et al., ibid., p. 1955; H. Choe et al., Cell 85, 1135 (1996); B. J. Doranz et al., ibid., p. 1149; H Deng et al., Nature 381, 661 (1996) ; T, Dragic et al. ibid., p. 667; J. F, Berson et al., J. Virol. 70, 6288 (1996).
14. H. Golding et al., J. Virol. 68, 1962 (1994).
15. H. Golding et al., ibid. 69, 6140 (1995).
16. 2-terminal cysteine residue, using m-maleimidobenzoyl-N-hydroxysuccinimide ester as the crosslinking agent. New Zealand White rabbits were immunized with the branched peptide or with KLH linear peptide at 1 mg per dose, emulsified in complete Freund's adjuvant followed by two to three boosts (3 weeks apart) in incomplete Freund's adjuvant. Immune and preimmune IgG fractions were prepared by two rounds of saturated ammonium sulfate precipitations.
17. 2-terminus of CCR-5. These peptide-treated immune IgGs were used in protein immunoblots. Complete blocking of binding to the fusin band was obtained with the fusin-derived peptide. The CCR-5-denved peptide had no blocking effect. The experiment was repeated twice.
18. R. Horuk, Immunol. Today 15, 169 (1994).
19. D. S. Dimitrov, Nature Med. 2, 640 (1996).
20. Abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, lie; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
21. C. K. Lapharr et al., data not shown.
22. Human A2.01.CD4.401 and mouse 3T3.CD4.401 cell lines expressing tailless CD4 molecules were produced in the laboratory of D. Littman. We thank C. C. Broder for helpful discussions, C. C. Broder and E A. Berger for providing us with vCBFY1, R. Blackburn for the generation of rabbit immune antisera to fusin and purified IgG, J. Manischewitz for cell line propagation and vaccinia virus infections, and B. Golding and K. Peden for critical review of the manuscript.