Characterization of catalytic centre mutants of macrophage migration inhibitory factor (MIF) and comparison to Cys81Ser MIF

European Journal of Biochemistry

Volumn 261, Issue 3, 1999, Pages 753-766

  Kleemann, Robert ^b   Kapurniotu, Aphrodite ^b   Mischke, Ralf ^b   Held, Jürgen ^b   Bernhagen, Jürgen ^a  

^a UNIVERSITY OF STUTTGART   (Germany)

^b NONE

Author keywords

Cysteine mutants; Cytokine; Disulfide; Inclusion body; Macrophage migration inhibitory factor

Indexed keywords

ALANINE; CYSTEINE; GLYCINE; GUANIDINE; HISTIDINE; LEUCINE; MACROPHAGE MIGRATION INHIBITION FACTOR; MUTANT PROTEIN; ORGANIC SOLVENT; OXIDOREDUCTASE; PROLINE; PROTEIN DISULFIDE ISOMERASE; SERINE; THIOREDOXIN;

AMINO ACID SUBSTITUTION; ARTICLE; CELL INCLUSION; CIRCULAR DICHROISM; DISULFIDE BOND; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME INACTIVATION; ENZYME MECHANISM; ENZYME STABILITY; HUMAN; IONIC STRENGTH; OXIDATION REDUCTION STATE; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; ULTRAVIOLET SPECTROPHOTOMETRY;

BASE SEQUENCE; CATALYTIC DOMAIN; CIRCULAR DICHROISM; CYSTEINE; DISULFIDES; DNA PRIMERS; HUMANS; MACROPHAGE MIGRATION-INHIBITORY FACTORS; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; SERINE; SPECTROPHOTOMETRY, ULTRAVIOLET; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 0347061957     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00327.x     Document Type: Article

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