DNA shuffling method for generating estrogen receptor α and β chimeras in yeast

BioTechniques

Volumn 34, Issue 2, 2003, Pages 278-288

  Sun, Jun ^a   Katzenellenbogen, John A ^a   Zhao, Huimin ^a   Katzenellenbogen, Benita S ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA SEQUENCES; GENETIC ENGINEERING; YEAST;

LIGANDS;

DNA;

ESTROGEN RECEPTOR ALPHA; ESTROGEN RECEPTOR BETA; FUNGAL DNA; GENE PRODUCT; RECEPTOR SUBTYPE;

3' UNTRANSLATED REGION; ARTICLE; BINDING SITE; CHIMERA; CROSSING OVER; EXPRESSION VECTOR; GENE AMPLIFICATION; GENE CONSTRUCT; GENE SEQUENCE; LIGAND BINDING; MOLECULAR BIOLOGY; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN DOMAIN; PROTEIN SYNTHESIS; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; TRANSACTIVATION; YEAST;

EID: 0346668140     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/03342st02     Document Type: Article

References (26)

1. Coco, W.M., W.E. Levinson, M.J. Crist, H.J. Hektor, A. Darzins, P.T. Pienkos, C.H. Squires, and D.J. Monticello. 2001. DNA shuffling method for generating highly recombined genes and evolved enzymes. Nat. Biotechnol. 19:354-359.
2. Gietz, R.D., R.H. Schiestl, A.R. Willems, and R.A. Woods. 1995. Studies on the transformation of intact yeast cells by the LiAc/SSDNA/PEG procedure. Yeast 11:355-360.
3. Giver, L., A. Gershenson, P.O. Freskgard, and F.H. Arnold. 1998. Directed evolution of a thermostable esterase. Proc. Natl. Acad. Sci. USA 95:12809-12813.
4. Hansson, L.O. and B. Mannervik. 2000. Use of chimeras generated by DNA shuffling: Probing structure-function relationships among glutathione transferases, p. 463-477. In Applications of Chimeric Genes and Hybrid Proteins, Pt C.
5. Judo, M.S.B., A.B. Wedel, and C. Wilson. 1998. Stimulation and suppression of PCR-mediated recombination. Nucleic Acids Res. 26:1819-1825.
6. Kikuchi, M., K. Ohnishi, and S. Harayama. 1999. Novel family shuffling methods for the in vitro evolution of enzymes. Gene 236:159-167.
7. Kikuchi, M., K. Ohnishi, and S. Harayama. 2000. An effective family shuffling method using single-stranded DNA. Gene 243:133-137.
8. Ma, H., S. Kunes, P.J. Schatz, and D. Botstein. 1987. Plasmid construction by homologous recombination in yeast. Gene 58:201-216.
9. McInerney, E.M., K.E. Weis, J. Sun, S. Mosselman, and B.S. Katzenellenbogen. 1998. Transcription activation by the human estrogen receptor subtype β (ERβ) studied with ERβ and ERα receptor chimeras. Endocrinology 139:4513-4522.
10. Mosselman, S., J. Polman, and R. Dijkema. 1996. ERβ: identification and characterization of a novel human estrogen receptor. FEBS Lett. 392:49-53.
11. Oldenburg, K.R., K.T. Vo, S. Michaelis, and C. Paddon. 1997. Recombination-mediated PCR-directed plasmid construction in vivo in yeast. Nucleic Acids Res. 25:451-452.
12. Ostermeier, M., J.H. Shim, and S.J. Benkovic. 1999. A combinatorial approach to hybrid enzymes independent of DNA homology. Nat. Biotechnol. 17:1205-1209.
13. Pompon, D. and A. Nicolas. 1989. Protein engineering by cDNA recombination in yeasts: shuffling of mammalian cytochrome P-450 functions. Gene 83:15-24.
14. Raymond, C.K., T.A. Pownder, and S.L. Sexson. 1999. General method for plasmid construction using homologous recombination. BioTechniques 26:134-141.
15. Rehberg, E., B. Kelder, E.G. Hoal, and S. Pestka. 1982. Specific molecular activities of recombinant and hybrid leukocyte interferons. J. Biol. Chem. 257:11497-11502.
16. Sieber, V., C.A. Martinez, and F.H. Arnold. 2001. Libraries of hybrid proteins from distantly related sequences. Nat. Biotechnol. 19:456-460.
17. Stemmer, W.P.C. 1994. DNA shuffling by random fragmentation and reassembly-invitro recombination for molecular evolution. Proc. Natl. Acad. Sci. USA 91:10747-10751.
18. Stemmer, W.P.C. 1994. Rapid evolution of a protein in-vitro by DNA shuffling. Nature 370:389-391.
19. Streuli, M., A. Hall, W. Boll, W.E. Stewart, II, S. Nagata, and C. Weissmann. 1981. Target cell specificity of two species of human interferon-α produced in Escherichia coli and of hybrid molecules derived from them. Proc. Natl. Acad. Sci. USA 78:2848-2852.
20. Sun, J., J. Baudry, J.A. Katzenellenbogen, and B.S. Katzenellenbogen. Molecular basis for the subtype discrimination of the estrogen receptor β selective ligand, diarylpropionitrile. Mol. Endocrinol. doi: 10.1210/me.2002-0341.
21. Volkov, A.A. and F.H. Arnold. 2000. Methods for in vitro DNA recombination and random chimeragenesis. Methods Enzymol. 328:447-456.
22. Wrenn, C.K. and B.S. Katzenellenbogen. 1993. Structure-function analysis of the hormone-binding domain of the human estrogenreceptor by region-specific mutagenesis and phenotypic screening in yeast. J. Biol. Chem. 268:24089-24098.
23. Zha, W., T. Zhu, and H. Zhao. Family shuffling with single-stranded DNA. In F.H. Arnold and G. Georgiou (Eds.), Methods in Molecular Biology, Directed Evolution Library Creation: Methods and Protocols. Humana Press, Totowa, NJ. (In Press.)
24. Zhang, J.H., G. Dawes, and W.P.C. Stemmer. 1997. Directed evolution of a fucosidase from a galactosidase by DNA shuffling and screening. Proc. Natl. Acad. Sci. USA 94:4504-4509.
25. Zhao, H. and F.H. Arnold. 1997. Functional and nonfunctional mutations distinguished by random recombination of homologous genes. Proc. Natl. Acad. Sci. USA 94:7997-8000.
26. Zhao, H., L. Giver, Z.X. Shao, J.A. Affholter, and F.H. Arnold. 1998. Molecular evolution by staggered extension process (StEP) in vitro recombination. Nat. Biotechnol. 16:258-261.