An engineered blockage within the ammonia tunnel of carbamoyl phosphate synthetase prevents the use of glutamine as a substrate but not ammonia

Biochemistry

Volumn 39, Issue 12, 2000, Pages 3240-3247

  Huang, Xinyi ^a   Raushel, Frank M ^a  

^a TEXAS A AND M UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMMONIA; CARBAMOYL PHOSPHATE SYNTHASE; GLUTAMINE;

ARTICLE; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME KINETICS; ENZYME MECHANISM; ENZYME PHOSPHORYLATION; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN MODIFICATION;

ALANINE; AMMONIA; ASPARTIC ACID; CARBAMOYL-PHOSPHATE SYNTHASE (GLUTAMINE-HYDROLYZING); CARBAMYL PHOSPHATE; ESCHERICHIA COLI; GLUTAMIC ACID; GLUTAMINE; GLYCINE; KINETICS; LYSINE; METHIONINE; MUTAGENESIS, SITE-DIRECTED; PHENYLALANINE; SERINE; SUBSTRATE SPECIFICITY; TIME FACTORS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0034724435     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi9926173     Document Type: Article

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