Kinetic characterization and X-ray structure of a mutant of haloalkane dehalogenase with higher catalytic activity and modified substrate range

Biochemistry

Volumn 35, Issue 40, 1996, Pages 13186-13195

  Schanstra, Joost P ^a   Ridder, Ivo S ^a   Heimeriks, Gaston J ^a   Rink, Rick ^a   Poelarends, Gerrit J ^a   Kalk, Kor H ^a   Dijkstra, Bauke W ^a   Janssen, Dick B ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ALKANE; OXIDOREDUCTASE;

ARTICLE; CONFORMATIONAL TRANSITION; DISSOCIATION; ENZYME ACTIVE SITE; ENZYME KINETICS; ENZYME MODIFICATION; HYDROLYSIS; HYDROPHOBICITY; ISOMERISM; NONHUMAN; PRIORITY JOURNAL; PROTEIN STABILITY; X RAY CRYSTALLOGRAPHY;

AMINO ACIDS; BACTERIA; BROMIDES; CHLORIDES; CRYSTALLOGRAPHY, X-RAY; ETHYLENE DIBROMIDE; HELIX-LOOP-HELIX MOTIFS; HEXANES; HYDROCARBONS, CHLORINATED; HYDROLASES; KINETICS; MODELS, MOLECULAR; MOLECULAR STRUCTURE; MUTAGENESIS, SITE-DIRECTED; PHENYLALANINE; PROTEIN BINDING; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

FELIS CATUS;

EID: 0029800609     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi961151a     Document Type: Article

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