Role of arginine 292 in the catalytic activity of chondroitin AC lyase from Flavobacterium heparinum

Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology

Volumn 1597, Issue 2, 2002, Pages 260-270

  Capila, Ishan ^a   Wu, Yi ^b   Rethwisch, David W ^b   Matte, Allan ^c   Cygler, Miroslaw ^c   Linhardt, Robert J ^a,b,d  

^a UNIVERSITY OF IOWA   (United States)

^b IA United States   (United States)

^c NATIONAL RESEARCH COUNCIL CANADA   (Canada)

^d E11 SSH   (United States)

Author keywords

Arginine 292; Chondroiotin AC lyase; Flavobacterium heparinum

Indexed keywords

ARGININE; CHONDROITIN ABC LYASE; CHONDROITIN SULFATE; GALACTOSAMINE; OLIGOSACCHARIDE;

ARTICLE; BINDING AFFINITY; BINDING SITE; CATALYSIS; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME KINETICS; FLAVOBACTERIUM; GEL ELECTROPHORESIS; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; VISCOSITY;

AMINO ACID SUBSTITUTION; ARGININE; BASE SEQUENCE; CARBOHYDRATE SEQUENCE; CATALYTIC DOMAIN; CHONDROITIN LYASES; CHONDROITIN SULFATES; DNA, BACTERIAL; FLAVOBACTERIUM; KINETICS; MODELS, CHEMICAL; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; VISCOSITY;

FLAVOBACTERIUM; PEDOBACTER HEPARINUS;

EID: 0037014051     PISSN: 01674838     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-4838(02)00304-7     Document Type: Article

References (44)

1. Polysaccharide lyases
2. Purification and properties of bacterial chondroitinases and chondrosulfatases
3. Search for the heparin antithrombin III-binding site precursor
4. Homogeneous, structurally defined heparin-oligosaccharides with low anticoagulant activity inhibit the generation of the amplification pathway C3 convertase in vitro
5. Differential anticoagulant activity of heparin fragments prepared using microbial heparinase
6. Isolation and characterization of human heparin
7. Electrophoresis and detection of nanogram quantities of exogenous and endogenous glycosaminoglycans in biological fluids
8. An enzymatic system for removing heparin in extracorporeal therapy
9. Structure-function relationships of beta-D-glucan endo- and exohydrolases from higher plants
10. High resolution structural analyses of mutant chitin A complexes with substrates provide new insight into the mechanism of catalysis
11. Action pattern of polysaccharide lyases on glycosaminoglycans
12. Alginate-modifying enzymes: A proposed unified mechanism of action for the lyases and epimerases
13. The calcium-binding sites of heparinase I from Flavobacterium heparinum are essential for enzymatic activity
14. Biochemical investigations and mapping of the calcium-binding sites of heparinase I from Flavobacterium heparinum
15. Heparinase I from Flavobacterium heparinum: Mapping and characterization of the heparin binding domain
16. Heparinase I from Flavobacterium heparinum: Role of positive charge in enzymatic activity
17. Histidine 295 and histidine 510 are crucial for the enzymatic degradation of heparan sulfate by heparinase III
18. Heparinase II from Flavobacterium heparinum: Role of cysteine in enzymatic activity as probed by chemical modification and site-directed mutagenesis
19. Heparinase II from Flavobacterium heparinum: Role of histidine residues in enzymatic activity as probed by chemical modification and site-directed mutagenesis
20. Heparinase I from Flavobacterium heparinum: Identification of a critical histidine residue essential for catalysis as probed by chemical modification and site-directed mutagenesis
21. Structure of a plant cell wall fragment complexed to pectate lyase C
22. Crystal structure of chondroitinase B from Flavobacterium heparinum and its complex with a disaccharide product at 1.7 Å resolution
23. Structural basis of hyaluronan degradation by Streptococcus pneumoniae hyaluronate lyase
24. Comparison of the activity of two chondroitin AC lyases on dermatan sulfate
25. Crystal structure of chondroitin AC lyase, a representative of a family of glycosaminoglycan degrading enzymes
26. Active site of chondroitin AC lyase revealed by the structure of enzyme-oligosaccharide complexes and mutagenesis
27. Isolation and expression in Escherichia coli of cslA and clsB, genes coding for the chondroitin sulfate-degrading enzymes chondroitinase AC and chondroitinase B, respectively, from Flavobacterium heparinum
28. Development of a genetic system for the transfer of DNA into Flavobacterium heparinum
29. Purification, characterization and specificity of chondroitin lyases and glucuronidase from Flavobacterium heparinum
30. Gradient polyacrylamide gel electrophoresis for determination of the molecular weight of heparin and low molecular weight heparin derivatives
31. Recombinant expression, purification, and kinetic characterization of chondroitinase AC and chondroitinase B from Flavobacterium heparinum
32. Physicochemical studies of fractionated bovine heparin: I. Some dilute solution properties
33. Physicochemical studies of fractionated bovine heparin: II. Viscosity as a function of ionic strength
34. Mode of action of heparin lyase on heparin
35. Preparation and structural characterization of large heparin-derived oligosaccharides
36. Comparison of the activity of two chondroitin AC lyases on dermatan sulfate
37. Study of structurally defined oligosaccharide substrates of heparin and heparan monosulfate lyases
38. Nomenclature for sugar-binding subsites in glycosyl hydrolases
39. Trichoderma reesei cellobiohydrolases: Why so efficient on crystalline cellulose?
40. Crystal structures of the cellulase Cel48F in complex with inhibitors and substrates give insights into its processive action
41. Xylan binding subsite mapping in the xylanase from Penicillium simplicissimum using xylooligosaccharides as cryo-protectant
42. Mechanism of hyaluronan binding and degradation: Structure of Streptococcus pneumoniae hyaluronate lyase in complex with hyaluronic acid disaccharide at 1.7 Å resolution