3-Nitrobenzanthrone (3-NBA) induced micronucleus formation and DNA damage in human hepatoma (HepG2) cells

Toxicology Letters

Volumn 146, Issue 2, 2004, Pages 103-109

  Lamy, Evelyn ^a   Kassie, Fekadu ^a   Gminski, Richard ^b   Schmeiser, Heinz H ^c   Mersch Sundermann, Volker ^a  

^a JUSTUS LIEBIG UNIVERSITY   (Germany)

^b CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^c GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

Author keywords

3 Nitrobenzanthrone; Comet assay; HepG2 cell; Micronucleus assay

Indexed keywords

3 NITROBENZANTHRONE; BENZENE DERIVATIVE; DNA; UNCLASSIFIED DRUG;

ARTICLE; CELL LINE; CELL MIGRATION; COMET ASSAY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DNA DAMAGE; GENOTOXICITY; HEPATOMA CELL; HUMAN; HUMAN CELL; MICRONUCLEUS; PRIORITY JOURNAL;

SALMONELLA;

EID: 0345708287     PISSN: 03784274     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.toxlet.2003.07.001     Document Type: Article

References (30)

1. Adachi, S., Kawamura, K., Takemoto, K., Suzuki, H., Hisamatsu, Y., 1999. In: Proceedings of the 7th International Inhalation Symposium: Relationship Between Acute and Chronic Effects of Air Pollution, Hannover Medical School, Book of Abstracts 67, A44.
2. Arlt V.M., Bieler C.A., Mier W., Wiessler M., Schmeiser H.H. DNA adduct formation by the ubiquitous environmental contaminant 3-nitrobenzanthrone in rats determined by (32)P-postlabeling. Int. J. Cancer. 193:2001;450-454.
3. Arlt V.M., Glatt H., Muckel E., Pabel U., Sorg B.L., Schmeiser H.H., Phillips D.H. Metabolic activation of the environmental contaminant 3-nitrobenzanthrone by human acetyltransferases and sulfotransferases. Carcinogenesis. 23:2002;1937-1945.
4. Arlt V.M., Sorg B.L., Osborne M., Hewer A., Seidel A., Schmeiser H.H., Phillips H.H. DNA adduct formation by the ubiquitous environmental pollutant 3-nitrobenzanthroen and its metabolites in rats. Biochem. Biophys. Res. Commun. 300:2003a;107-114.
5. Arlt V.M., Stiborova M., Hewer A., Schmeiser H.H., Phillips D.H. Human enzymes involved in the metabolic activation of the environmental contaminant 3-nitrobenzanthrone: Evidence for reductive activation by human NADPH: cytochrome P450 reductase. Cancer Res. 63:2003b;2752-2761.
6. Bieler C.A., Wiessler M., Erdinger L., Suzuki H., Enya T., Schmeiser H.H. DNA adduct formation from the mutagenic air pollutant 3-nitrobenzanthrone. Mutat. Res. 439:1999;307-311.
7. Borlak J., Hansen T., Yuan Z.X., Sikka H.C., Kumar S., Schmidbauer S., Frank H., Jacob J., Seidel A. Metabolism and DNA-binding of 3-nitrobenzanthrone in primary rat alveolar type II cells, in human fetal bronchial rat epithelial and mesenchymal cell lines. Polycyclic Arom. Comp. 21:2000;73-86.
8. Enya T., Suzuki H., Watanabe T., Hirayama T., Hisamatsu Y. 3-Nitrobenzanthrone, a powerful bacterial mutagen and suspected human carcinogen found in diesel exhaust and airborne particulates. Environ. Sci. Technol. 31:1997;2772-2776.
9. Eastmond D.A., Tucker J.D. Identification of aneuploidy-inducing agents using cytokinesis-blocked human lymphocytes and anti-kinetochore antibody. Environ. Mol. Mutagen. 13:1989;34-43.
10. Fenech M. The cytokinesis-block micronucleus technique: a detailed description of the method and its application to genotoxicity studies in human populations. Mutat. Res. 285:1993;35-44.
11. Fenech M. The advantages and disadvantages of cytokinesis-block micronucleus method. Mutat. Res. 392:1997;11-18.
12. Fenech M. The in vitro micronucleus technique. Mutat. Res. 455:2000;81-95.
13. Garriott M.L., Phelps J.B., Hoffman F.W. A protocol for the in vitro micronucleus test. I. Contributions to the development of a protocol suitable for regulatory submissions from an examination of 16 chemicals with different mechanisms of action and different levels of activity. Mutat. Res. 517:2002;123-134.
14. International Agency for Research on Cancer (IARC), 1989. IARC monograph on the evaluation of carcinogenic risks to humans. Diesel and Gasoline Engine Exhaust and Some Nitroarenes, Vol. 46. Lyon, France, pp. 1-458.
15. Kawanishi M., Enya T., Suzuki H., Takebe H., Matsui S., Yagi T. Postlabelling analysis of DNA adducts formed in human hepatoma cells treated with 3-nitrobenzanthrone. Mutat. Res. 470:2000;133-139.
16. Kirsch-Volders M., Sofuni T., Aardema M., Albertini S., Eastmond D., Fenech M., Ishidate M., Lorge E., Norppa H., Surralles J., Von der Hude W., Wakata A. Report from the in vitro micronucleus assay working group. Environ. Mol. Mutagen. 35:2000;167-172.
17. Klaude M., Eriksson S., Nygren J., Ahnstrom G. The comet assay: mechanisms and technical considerations. Mutat. Res.363:1996;89-96.
18. Knasmuller S., Parzefall W., Sanyal R., Ecker S., Schwab C., Uhl M., Mersch-Sundermann V., Williamson G., Hietsch G., Langer T., Darroudi F., Natarajan A.T. Use of metabolically competent human hepatoma cells for the detection of mutagens and antimutagens. Mutat. Res. 402:1998;185-202.
19. Lu W.Q., Chen X.N., Yue F., Jenter C., Gminski R., Li X.X., Xie H., Mersch-Sundermann V. Studies on the in vivo and in vitro mutagenicity and the lipid peroxidation of chlorinated surface (drinking) water in rats and metabolically competent human cell. Mutat. Res. 513:2002;151-157.
20. Marzin D. The position of the in vitro micronucleus test within the battery of screening for genotoxic potential determination and regulatory guidelines. Mutat. Res. 392:1997;175-181.
21. Natarajan A.T., Darroudi F. Use of human hepatoma cells for in vitro metabolic activation of chemical mutagens/carcinogens. Mutagenesis. 6:1991;399-403.
22. Olive P.L., Wlodek D., Durand R.E., Banath J.P. Factors influencing DNA migration from individual cells subjected to gel electrophoresis. Exp. Cell Res. 198:1992;259-267.
23. Phousongphouang P.T., Grosovsky A.J., Eastmond D.A., Covarrubias M., Arey J. The genotoxicity of 3-nitrobenzanthrone and the nitropyrene lactones in human lymphoblasts. Mutat. Res. 472:2000;93-103.
24. Seidel A., Dahmann D., Krekeler H., Jocob J. Biomonitoring of polycyclic aromatic compounds in the urine of mining workers occupationally exposed to diesel exhaust. Int. J. Hyg. Environ. Health. 204:2002;333-338.
25. Silvers K.J., Couch L.H., Rorke E.A., Howard P.C. Role of nitroreductases but not cytochromes P450 in the metabolic activation of 1-nitropyrene in the HepG2 cell line. Biochem. Pharmacol. 54:1997;927-936.
26. Singh N.P., McCoy M.T., Tice R.R., Schneider E.L. A microgel technique for the direct quantification of DNA damage and repair in individual fibroblasts cultured on microscope slides. Mutation Res. 252:1991;175-184.
27. Tice R.R., Andrews P.W., Singh N.P. The single cell gel assay: a sensitive technique for evaluating intercellular differences in DNA damage and repair. Basic Life Sci. 53:1990;291-301.
28. Tice R.R., Agurell E., Anderson D., Burlinson B., Hartmann A., Kobayashi H., Miyamae Y., Rojas E., Ryu J.C., Sasaki Y.F. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ. Mol. Mutagen. 35:2000;206-221.
29. Uhl M., Helma C., Knasmüller S. Evaluation of the single gel electrophoresis assay with human hepatoma (HepG2) cells. Mutat Res. 468:2000;213-225.
30. World Health Organization (WHO), 1996. Diesel fule and exhaust emissions, International Program on Chemical Safety. Environmental Health Criteria Series, No. 171, World Health Organization, Geneva, Switzerland.