Use of metabolically competent human hepatoma cells for the detection of mutagens and antimutagens

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

Volumn 402, Issue 1-2, 1998, Pages 185-202

  Knasmüller, Siegfried ^a   Parzefall, Wolfram ^a   Sanyal, Ratna ^a   Ecker, Sonja ^a   Schwab, Christina ^a   Uhl, Maria ^a   Mersch Sundermann, Volker ^b   Williamson, Gary ^c   Hietsch, Gerhard ^a   Langer, Theo ^d   Darroudi, Firouz ^e   Natarajan, Adayapalam T ^e  

^a UNIVERSITY OF VIENNA   (Austria)

^b UNIVERSITY OF HEIDELBERG   (Germany)

^c INSTITUTE OF FOOD RESEARCH   (United Kingdom)

^d UNIVERSITY OF VETERINARY MEDICINE   (Austria)

^e LEIDEN UNIVERSITY   (Netherlands)

Author keywords

Antimutagen; Human Hep G2 cell; Metabolic activation; Mutagenecity

Indexed keywords

ANTIMUTAGENIC AGENT; MUTAGENIC AGENT;

CELL STRAIN HEPG2; CONFERENCE PAPER; ENZYME INDUCTION; ENZYME INHIBITION; GENOTOXICITY; HUMAN; HUMAN CELL; MICRONUCLEUS TEST; MUTAGEN TESTING; MUTAGENICITY; PRIORITY JOURNAL;

ANIMALS; ANTIMUTAGENIC AGENTS; CARCINOMA, HEPATOCELLULAR; HUMANS; LIVER NEOPLASMS; MUTAGENICITY TESTS; MUTAGENS; TUMOR CELLS, CULTURED;

EID: 0345296419     PISSN: 00275107     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0027-5107(97)00297-2     Document Type: Conference Paper

References (120)

1. [1] C.A.H. Bigger, J.E. Tomaszewski, A. Dipple, R.S. Lake, Limitations of metabolic activation systems used with in vitro tests for carcinogens, Science 209 (1980) 503-505.
2. [2] R.P. Bos, J.M. Neis, P.J.L. van Gemert, P.T. Henderson, Mutagenicity testing with the Salmonella/hepatocyte and Salmonella/microsome assays: a comparative study with some known genotoxic compounds, Mutation Res. 124 (1983) 103-112.
3. [3] J. Ashby, The significance and interpretation of in vitro mutagenicity results, in: K.H. Norpoth, R.C. Garner (Eds.), Short Term Tests for Detecting Carcinogens, Springer, Berlin, 1980, pp. 74-93.
4. [4] R.W. Tennant, B.H. Margolin, M.D. Shelby, E. Zeiger, J.K. Haseman, J. Spalding, W. Caspary, M. Resnick, S. Stasiewicz, B. Anderson, R. Minor, Prediction of chemical carcinogenicity in rodents from in vitro genetic toxicity assays, Science 236 (1987) 933-941.
5. [5] D. Utesch, H.R. Glatt, F. Oesch, Rat hepatocyte mediated bacterial mutagenicity in relation to carcinogenicity of benz(a)anthracene, benzo(a)pyrene and 25 methylated derivatives, Cancer Res. 47 (1987) 1509-1515.
6. [6] J. Rueff, C. Chiapella, K.J. Chipman, F. Darroudi, I. Duarte Silva, M. Duverger van Bogaert, E. Fonti, H.R. Glatt, P. Isern, A. Laires, A. Leonard, M. Llangostera, P. Mossesso, A.T. Natarajan, F. Palitti, A.S. Rodrigues, A. Schinoppi, G. Turchi, G. Wehrle-Schneider, Development and validation of alternative metabolic systems for mutagenicity testing in short term assays, Mutation Res. 353 (1996) 151-176.
7. [7] G. Brambilla, A. Martelli, Human hepatocytes in genotoxicity assays, Pharmacol. Res. 22 (1990) 381-392.
8. [8] B.E. Butterworth, T. Smith Oliver, L. Earle, D.L. Lowry, R.D. White, D.L. Doolittle, P.K. Working, R.C. Cattley, R. Jirtle, Michalopoulos, S. Strom, Use of primary cultures of human hepatocytes in toxicology studies, Cancer Res. 49 (1989) 1075-1084.
9. [9] K. Müller-Teggethoff, P. Kasper, L. Müller, Evaluation studies on the in vitro hepatocyte micronucleus assay, Mutation Res. 335 (1995) 293-307.
10. [10] P.M. Eckl, S.C. Strom, G. Michalopoulos, R.J. Jirtle, Induction of sister chromatid exchanges in cultures of adult rat hepatocytes by directly and indirectly acting mutagens/carcinogens, Carcinogenesis 8 (1987) 1077-1083.
11. [11] C. Salissidis, U. Kulka, E. Schmied, D. Paul, M. Bauchinger, Induction of chromosome aberration and sister chromatid exchange by indirectly acting mutagens in immortal mouse and rat hepatocyte lines, Mutation Res. 207 (1991) 111-115.
12. [12] C. Guguen-Guillouzo, A. Guillouzo, Modulation of functional activities in cultured rat hepatocytes, Mol. Cell. Biochem. 53/54 (1983) 35-56.
13. [13] S.C. Strom, R.L. Jirtle, R.S. Jones, D.L. Novicki, M.R. Rosenberg, A. Novotny, G. Irons, G. Michalopoulos, Isolation, culture, and transplantation of human hepatocytes, J. Natl. Cancer Inst. 68 (1982) 771-778.
14. 5 and P450 in primary cultures of parenchymal liver cells on collagen membranes, Life Sci. 18 (1976) 1139-1144.
15. [15] C.L. Crespi, Use of genetically altered cells for genetic toxicology, in: D.H. Phillips, S. Venitt (Eds.), Environ. Mutagen., 1995, pp. 233-260.
16. [16] J. Doehmer, S. Dogra, T. Friedberg, S. Manier, M. Adesnik, H. Glatt, F. Oesch, Stable expression of rat cytochrome P450B1cDNA in Chinese hamster cells (V-79) and metabolic activation of aflatoxin B1, Proc. Natl. Acad. Sci. U.S.A. 85 (1988) 5769-5773.
17. [17] J. Doehmer, A. Seidel, B.H. Glatt, H. Molders, P. Siegert, T. Friedberg, F. Oesch, Stable expression of rat cytochrome P4501A1cDNA in V79 Chinese hamster cells and their use in mutagenicity testing, Mol. Pharmacol. 37 (1990) 608-613.
18. [18] U. Kulka, J. Doehmer, H.R. Glatt, M. Bauchinger, Cytogenetic effects of promutagens in genetically engineered V-79 Chinese hamster cells expressing cytochromes P450, Eur. J. Pharmacol. 288 (1993) 299-304.
19. [19] A.S. Rodrigues, I.D. Silva, M.H. Caria, A. Laires, T. Chaveca, H.R. Glatt, J. Rueff, Genotoxicity assessment of aromatic amines and amides in genetically engineered V-79 cells, Mutation Res. 341 (1994) 93-100.
20. [20] E. Roscher, F.J. Wiebel, Mutagenicity, clastogenicity and cytotoxicity of procarcinogens in a rat hepatoma cell line competent for xenobiotic metabolism, Mutagenesis 3 (1988) 269-276.
21. [21] R. Dean, G.L. Bynum, D. Kram, E. Schneider, Sister chromatid exchange induction by carcinogens in Reuber H4IIE hepatoma cells: an in vitro system which does not require the addition of activating factors, Mutation Res. 74 (1980) 477-483.
22. [22] R. Dean, G.L. Bynum, D. Jacobson-Kram, E. Hadley, Activation of polycyclic aromatic hydrocarbons in Reuber H4IIE hepatoma cells: an in vitro system for the induction of SCEs, Mutation Res. 111 (1983) 419-427.
23. [23] C. Tong, S. VetBrat, G.M. Williams, Sister chromatid exchange induction by polycyclic aromatic hydrocarbons in an intact cell system of adult rat liver epithelial cells, Mutation Res. 91 (1981) 467-473.
24. [24] C. Tong, S. Telang, G.M. Williams, Differences in responses of four adult rat liver cell lines to a spectrum of chemical carcinogens, Mutation Res. 130 (1984) 53-61.
25. [25] R. Salvia, R. DeMeschini, M. Fiore, F. Palitti, M.A. Carcuccio, G. Turchi, Induction of SCEs by procarcinogens in metabolically competent Chinese hamster epithelial cells, Mutation Res. 207 (1988) 69-74.
26. [26] J.K. Selkirk, Divergence of metabolic activation systems for short term mutagenicity assays, Nature 270 (1977) 604-607.
27. [27] C.J. Maslansky, G.M. Williams, Primary cultures and the levels of cytochrome P450 in hepatocytes from mouse, rat, hamster and rabbit liver, In Vitro 18 (1982) 683-693.
28. [28] A.M. Pfeifer, K.E. Cole, D.T. Smoot, A. Weston, J.D. Groopman, P.G. Shields, J.M. Vignaud, M. Juillerat, M.M. Lipsky, B.F. Trump, Simian virus large tumor antigen immortalized normal liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens, Proc. Natl. Acad. Sci. U.S.A. 90 (1993) 5123-5127.
29. [29] D.P. Aden, A. Vogel, S. Plotkin, I. Damjanov, B.B. Knowles, Controlled synthesis of HBs Ag in a differentiated human liver carcinoma-derived cell line, Nature 282 (1979) 615-616.
30. [30] B.B. Knowles, C.C. Howe, D.P. Aden, Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen, Science 209 (1980) 497-499.
31. [31] A.T. Natarajan, F. Darroudi, Use of human hepatoma cells for in vitro metabolic activation of chemical mutagens/carcinogens, Mutagenesis 5 (1991) 399-403.
32. [32] T.J. Hall, G. Cambridge, P.R. James, Development of a co-culture system with induced Hep G2 an dK562 cells for examining drug metabolism in vitro, studies with cyclophosphamide, ondansetron and cisplatin, Res. Commun. Chem. Pathol. Pharmacol. 72 (1991) 161-168.
33. [33] G. Chang, D. Jacobson Kram, J.R. Williams, Use of an established human hepatoma cell line with endogenous bioactivation for gene mutation studies, Cell. Biol. Toxicol. 4 (1988) 267-279.
34. [34] C. Schwab, Einsatz einer metabolisch kompetenten menschlichen Hepatoma-Zellinie (Hep G2) und subzellulärerer Enzymfraktionen zur Untersuchung DNA-schädigender Effekte von heterozyklischen aromatischen Aminen, Thesis, Vienna, 1997.
35. [35] D. Gergel, V. Misik, K. Ondrial, A.I. Cederbaum, Increased cytotoxicity of 3-morpholinosydnonimine to Hep G2 cells in the presence of superoxide dismutase: role of hydrogen peroxide and iron, J. Biol. Chem. 270 (1995) 20927-20979.
36. [36] M.G. Neuman, G. Koren, C. Tiribelli, In vitro assessment of ethanol-induced hepatotoxicity on a Hep G2 line, Biochem. Biophys. Res. Commun. 197 (1993) 932-941.
37. [37] N.H. Shear, I.M. Malkiewicz, D. Klein, G. Koren, S. Randor, M.G. Neuman, Acetaminophen-induced toxicity to human epidermoid cell line A431 and hepatoblastoma cell line Hep G2 in vitro, is diminished by silymarin, Skin Pharmacol. 8 (1995) 6279-6291.
38. [38] C.M. Mangold, R. Streeck, Mutational analysis of cysteine residues in the hepatitis B virus small envelope protein, J. Virol. 67 (1993) 4588-4597.
39. [39] K. Gustin, M. Saphiro, M. Lee, R.D. Burk, Characterization of the role of individual protein binding motifs within the hepatitis B enhancer 1 on X promoter activity using linker scanning mutagenesis, Virology 193 (1993) 653-660.
40. [40] H.-L. Wu, P.-J. Chen, S.-J. Tu, M.-H. Lin, M.-Y. Lai, D.-S. Chen, Characterization and genetic analysis of alternatively spliced transcripts of hepatitis B virus in infected human liver tissues and transfected Hep G2 cells, J. Virol. 65 (1991) 1680-1686.
41. [41] R.A. Pimental, B. Liang, G.-K. Yee, A. Wilhelmsson, L. Poellinger, K.E. Paulson, Dioxin receptor and C/EBP regulate the function of the glutathione-S-transferase Ya gene xenobiotic response element, Mol. Cell. Biol. 13 (1993) 4365-4373.
42. [42] X.H. Wang, P.K.W. Harris, R.G. Ulrich, R.L. Voormann, Identification of dioxin responsive genes in Hep G2 cells using differential mRNA display RT-PCR, Biochem. Biophys. Res. Commun. 220 (1996) 784-788.
43. [43] D. Kardassis, V. Zannis, C. Cladaras, Organization of the regulatory elements and nuclear activities participating in the transcription of the human apolipoprotein B gene, J. Biol. Chem. 267 (1992) 2622-2632.
44. [44] L. Babajko, Transcriptional regulation of insulin-like growth factor binding protein 1 expression by insulin and cyclic AMP, Growth Regul. 5 (1995) 83-91.
45. [45] K. Rust, L. Bingle, W. Mariencheck, A. Persson, E.C. Crouch, Characterization of the human surfactant proteins D promoter, transcriptional regulation of SP-D gene expression by glucocorticoids Am, J. Respir. Cell Mol. Biol. 14 (1996) 121-130.
46. [46] C.H. Miao, W.-T. Ho, D.L. Greenberg, E.W. Davie, Transcriptional regulation of the gene coding for human protein C, J. Biol. Chem. 271 (1996) 9587-9594.
47. [47] S. Sassa, O. Sugita, R.A. Galbraith, A. Kappas, Drug metabolism by the human hepatoma cell line Hep G2, Biochem. Biophys. Res. Commun. 143 (1987) 52-57.
48. [48] L. Diamond, F. Kruszewski, D.P. Aden, B.B. Knowles, W.M. Baird, Metabolic activation of benzo(a)pyrene by a human hepatoma cell line, Carcinogenesis 1 (1980) 871-875.
49. [49] S. Limbosch, Benzo(a)pyrene-and aldrin-metabolizing activities in cultured human and rat hepatoma cell lines, J. Natl. Cancer Inst. 71 (1983) 281-286.
50. [50] E.P. Eddy, P.C. Howard, D. McCoy, H.S. Rosenkranz, Mutagenicity, unscheduled DNA-synthesis and metabolism of 1-nitropyrene in the human hepatoma cell line Hep G2, Cancer Res. 47 (1987) 3163-3168.
51. [51] M.H. Grant, S.J. Duthie, A.G. Gray, M.D. Burke, Mixed function oxidase and UDP-glucuronyltransferase activities in the human Hep G2 hepatoma cell line, Biochem. Pharmacol. 37 (1988) 4111-4116.
52. [52] H. Doostdar, S.J. Duthie, M.D. Burke, W.T. Melvin, M.H. Grant, The influence of culture medium composition on drug metabolizing enzyme activities of the human liver-derived Hep G2 cell line, FEBS Lett. 241 (1988) 15-18.
53. [53] O. Fardel, F. Morel, D. Ratanasavanh, A. Fautrel, P. Beaune, A. Guillouzo, Expression of drug metabolizing enzymes in human Hep G2 hepatoma cells, in: B. Clement, A. Guillouzo (Eds.), Cellular and Molecular Aspects of Cirrhosis, Vol. 216, John Libbey Eurotext, Montrouge, France, 1992, pp. 327-330.
54. [54] A.L. Roe, J.E. Snawder, R.W. Benson, D.W. Roberts, D.A. Casciano, Hep G2 cells: an in vitro model for P450-dependent metabolism of acetaminophen, Biochem. Biophys. Res. Commun. 190 (1993) 15-19.
55. [55] M. Duverger van Bogaert, P.J. Dierickx, C. Stecca, M.C. Crutzen, Metabolic activation by a supernatant from human hepatoma cells: a possible alternative in mutagenic tests, Mutation Res. 292 (1993) 199-204.
56. [56] J. DiGiovanni, J.M. Singer, L. Diamond, Comparison of the metabolic activation of 7,12-dimethylbenz(a)anthracene by a human hepatoma cell line (Hep G2) and low passage hamster embryo cells, Cancer Res. 44 (1984) 2878-2884.
57. [57] A.K. Jaiswal, P. Burnett, M. Adesnik, O.W. McBride, Nucleotide and deduced amino acid sequence of a human cDNA (NQO2) corresponding to a second member of the NAD(P)H:quinone oxidoreductase gene family: extensive polymorphism at the NQO2 gene locus on chromosome 6, Biochemistry 29 (1990) 1899-1906.
58. [58] K.L. Dearfield, D.K. Jacobson-Kram, N.A. Brown, J.R. Williams, Evaluation of a human hepatoma cell line as a target cell in genetic toxicology, Mutation Res. 108 (1983) 437-449.
59. 2+, Biochem. J. 314 (1996) 151-158.
60. [60] Y. Sakakibara, M. Suiko, M.C. Liu, De Novo sulfation of L-tyrosine in Hep G2 human hepatoma cells and its possible functional implication, Eur. J. Biochem. 226 (1994) 293-301.
61. [61] T. Walle, U.K. Walle, J.A. Shwed, K.R. Thornburg, C.E. Mathis, G.R. Pesola, Human phenol sulfotransferases: chiral substrates and expression in Hep G2 cells, Chem. Biol. Interact. 92 (1994) 47-55.
62. [62] E. Coroneos, E. Sim, Arylamine-N-acetyltransferase activity in human cultured cell lines, Biochem. J. 294 (1993) 481-486.
63. [63] H. Doostdar, M.H. Grant, W.T. Melvin, C.R. Wolf, M.D. Burke, The effects of inducing agents on cytochrome P450 and UDP-glucuronyltransferase activities in human Hep G2 hepatoma cells, Biochem. Pharmacol. 46 (1993) 629-635.
64. [64] R. Cameron, G.D. Sweeney, K. Jones, G. Lee, E. Farber, A relative deficiency of cytochrome P450 and aryl hydrocarbon [benzo(a)pyrene] hydroxylase in hyperblastic nodules induced by 2-acetylaminofluorene in rat liver, Cancer Res. 36 (1976) 3888-3893.
65. [65] L.G. Sultanos, E.S. Vesell, Enhanced drug metabolizing capacity within liver adjacent to human and rat liver tumors, Proc. Natl. Acad. Sci. U.S.A. 77 (1980) 600-603.
66. [66] H. Babich, M.K. Sardana, E. Borenfreund, Acute cytotoxicities of polynuclear aromatic hydrocarbons determined in vitro with the human liver tumor cell line, Hep G2, Cell. Biol. Toxicol. 4 (1988) 295-309.
67. [67] P. Labruzzo, X.F. Yu, M.J. Dufresne, Induction of aryl-hydrocarbon-hydroxylase and demonstration of a specific nuclear receptor for 2,3,7,8-tetrachloro-dibenzo-p-dioxin in two human hepatoma cell lines, Biochem. Pharmacol. 38 (1989) 2339-2348.
68. [68] M.A. Belisario, A.R. Arena, R. Pecce, R. Borgia, N. Staiano, F. De Lorenzo, Effect of enzyme inducers on metabolism of 1-nitropyrene in human hepatoma cell line Hep G2, Chem. Biol. Interact. 78 (1991) 253-268.
69. [69] S.J. Duthie, C.S. Coleman, M.H. Grant, Status of reduced glutathione in the human hepatoma cell line Hep G2, Biochem. Pharmacol. 37 (1988) 3365-3368.
70. [70] M.S. DuPont, N. Tawfiq, J.A. Pumb, S.C.D.R. Wanigatunga, R.K. Heaney, G. Williamson, S.R.R. Musk, J.R. Bacon, G.R. Fenwick, The application of cultured cell biochemical assays to the investigation of protective factors in plant foods, in: H. Koslowska, J. Fornal, Z. Zduncyk (Eds.), Bioactive Substances in Food of Plant Origin, Vol. 2, Polish Academy of Sciences, Olsztyn, Poland, 1994, pp. 508-512.
71. [71] M. Duverger van Bogaert, P.J. Dierickx, M.C. Crutzen, Mutagenic activation of aromatic amines by a human hepatoma cell (Hep G2) supernatant tested by means of Salmonella typhimurium strains with different acetyltransferase activities, Mutation Res. 325 (1995) 219-227.
72. [72] M. Watanabe, M. Ishidate, T. Nohmi, Sensitive method for the detection of mutagenic nitroarenes and aromatic amines: new derivatives of Salmonella typhimurium tester strains possessing elevated O-acetyltransferase levels, Mutation Res. 234 (1990) 337-348.
73. [73] F. Darroudi, A.T. Natarajan, Metabolic activation of chemicals to mutagenic carcinogens by human microsomal extracts in Chinese hamster ovary cells (in vitro), Mutagenesis 8 (1993) 11-15.
74. [74] F. Darroudi, A.T. Natarajan, Induction of sister chromatid exchanges, micronuclei and gene mutations by indirectly acting promutagens using human hepatoma cells as an activation system, ATLA 22 (1994) 445-453.
75. [75] D.M. Maron, B.N. Ames, Revised methods for the Salmonella mutagenicity test, Mutation Res. 113 (1983) 173-215.
76. [76] T. Yahagi, M. Nagao, Y. Scino, T. Matsushima, T. Sugimura, M. Okada, Mutagenicity of N-nitrosamines on Salmonella, Mutation Res. 48 (1977) 121-130.
77. [77] T.-K. Rao, J.-A. Jong, W. Lijinski, J.L. Epler, Mutagenic activity of aliphatic nitrosamines in Salmonella typhimurium, Mutation Res. 48 (66) (1979) 1-17.
78. [78] F. Darroudi, C.M. Meijers, V. Hadjidekova, A.T. Natarajan, Detection of aneugenic and clastogenic potential of X-rays and directly and indirectly acting chemicals in human hepatoma (Hep G2) cells and peripheral blood lymphocytes using the micronucleus assay and fluorescent in situ hybridization with DNA-centromeric probes, Mutagenesis 11 (1996) 425-433.
79. [79] Z. Zhou, D.A. Casciano, R.H. Heflich, Use of the human liver cell line Hep G2 in a modified Salmonella reversion assay, Cancer Lett. 32 (1986) 327-334.
80. [80] K. Lindahl-Kissling, T.S. Bhatt, I. Karlberg, M. Coombes, Frequency of sister chromatid exchanges in human lymphocytes cultivated with a human hepatoma line as an indicator of the carcinogenic potency of two cyclopenta(a)phenanthrenes, Carcinogenesis 5 (1984) 11-14.
81. [81] T.S. Bhatt, M. Coombs, J. Di Giovanni, L. Diamond, Mutagenesis in Chinese hamster cells by cyclopenta-(a)phenanthrenes activated by a human hepatoma cell line, Cancer Res. 43 (1983) 984-986.
82. [82] L. Diamond, K. Cherian, R.G. Harvey, J. DiGiovanni, Mutagenic activity of methyl-and fluoro-substituted derivates of polycyclic aromatic hydrocarbons in a human hepatoma (Hep G2) cell mediated assay, Mutation Res. 136 (1984) 65-72.
83. [83] C.J.M. Rompelberg, S.J.C.J. Evertz, G.C.D.M. Bruintjes-Rozier, P.D. van den Heuvel, H. Verhagen, Effects of eugenol on the genotoxicity of established mutagens in the liver, Food Chem. Toxicol. 34 (1996) 33-42.
84. [84] G. Brambilla-Campart, R. Canonero, E. Mereto, M. Ferro, Cytotoxic and genotoxic effects of 10 N-nitroso-compounds in a human hepatoma cell line (Hep G2): comparison with human hepatocyte cells, ATLA 17 (1989) 22-27.
85. [85] D.J. Grdina, P. Dale, R. Weichselbaumer, Protection against AZT-induced mutagenesis at the HGPRT locus in a human cell line by WR-151326, Int. J. Rad. Oncol. Biol. Phys. 22 (1992) 813-815.
86. [86] B.M. Hasspieler, F.N. Ali, M. Alipou, G.D. Haffner, K. Adeli, Human bio-assays to assess environmental genotoxicity: development of a DNA-break bioassay in Hep G2 cells, Clin. Biochem. 98 (1995) 113-116.
87. [87] T.-Y. Liu, T. Chao, S.-H. Chiang, C.-W. Chi, Differential sensitivity of human hepatoma cell line and primary rat hepatocyte culture to benzo(a) pyrene-induced unscheduled DNA synthesis and adduct formation, Cell Biol. Int. 17 (1993) 441-447.
88. [88] F. Naji-Ali, B.M. Hasspieler, D. Haffner, K. Adeli, Human bioassays to assess environmental genotoxicity: development of DNA repair assays in Hep G2 cells, Clin. Biochem. 27 (1994) 441-448.
89. [89] K.J. Silvers, E.P. Eddy, E.G. McCoy, H.S. Rosenkranz, P.C. Howard, Pathways for the mutagenesis of 1-nitropyrene and dinitropyrene in the human hepatoma cell line Hep G2, Environ. Health Perspect. 102 (1994) 195-200, Suppl. 6.
90. [90] M.K. Grady, D. Jacobson-Kram, K.L. Dearfield, J.R. Dearfield, J.R. Williams, Induction of sister chromatid exchanges by benzidine in rat and human hepatoma cell lines and inhibition by indomethacin, Cell. Biol. Toxicol. 2 (1986) 223-230.
91. [91] S.J. Santos, C.S. Takahashi, A.T. Natarajan, Cytogenetic effects of the antichagasic benzidazole on human cells in vitro, Mutation Res. 320 (1994) 305-314.
92. [92] K.L. Dearfield, D.K. Jacobson-Kram, B.E. Huber, J.R. Williams, Induction of sister chromatid exchanges in human and rat hepatoma cell lines by cyclophosphamide and phosphamide mustard and the effects of cytochrome P450 inhibitors, Biochem. Pharmacol. 35 (1986) 2199-2205.
93. [93] G.R. Mohn, S. Ecker, J. van der Stel, M.R.J. Hamzink, J.F.C. Stavenhuiter, C.F. van Kreijl, S. Knasmüller, Antimutagenic activity of bioflavonoid compounds including chrysin, against the mutagenicity of benzo(a)pyrene and the food pyrolysis product, PhIP, in Salmonella strains TA98/TA100 and in human Hep G2 cells, Mutagenesis, in press.
94. [94] D.M.F. Salvadori, L.R. Ribero, A.T. Natarajan, The anticlastogenicity of β-carotene evaluated on human hepatoma cells, Mutation Res. 303 (1993) 151-156.
95. [95] R. Sanyal, F. Darroudi, W. Parzefall, M. Nagao, S. Knasmüller, Inhibition of the genotoxic effects of heterocyclic amines in human-derived hepatoma cells by dietary bioantimutagens, Mutagenesis 12 (1997) 297-303.
96. [96] E. Roscher, F.J. Wiebel, Genotoxicity of 1,3-and 1,6-dinitropyrene: induction of micronuclei in a panel of mammalian test cell lines, Mutation Res. 278 (1992) 11-17.
97. [97] Diesel and Some Gasoline Engine Exhausts and Some Nitroarenes, IARC Monogr. Eval. Carcinog. Risks Hum., WHO, Lyon, 49 (1989) 201-246.
98. [98] D.W. Wild, W. Freser, S. Michel, H.L. Lord, P.D. Josephy, Metabolic activation of heterocyclic aromatic amines catalyzed by human arylamine-N-acetyltransferase isoenzymes (NAT1 and NAT2) expressed in Salmonella typhimurium, Carcinogenesis 16 (1995) 643-648.
99. [99] Yanagawa, M. Sawada, T. Deguchi, F.J. Gonzalez, T. Kamataki, Stable expression of human CYP1A2 and N-acetyltransferases in Chinese hamster CHL cells: mutagenic activation of 2-amino-3-methylimidazo[4,5-f]quinoline and 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline, Cancer Res. 54 (1994) 3422-3427.
100. [100] C. Schwab, R. Sanyal, S. Knasmüller, F. Darroudi, Einsatz metabolisch kompetenter menschlicher Leberzellen für Zytogenitätstests, 15th Annual Meeting of the German Mutagen Society, 1996, p. 79.
101. [101] D. Wild, E. Gocke, D. Harnasch, G. Kaiser, M.T. King, Differential mutagenic activity of IQ (2-amino-3-methylimidazo[4,5-f]quinoline) in Salmonella typhimurium strains in vitro and in vivo, in Drosophila and in mice, Mutation Res. 156 (1985) 93-102.
102. [102] N. Loprieno, G. Bonacristian, G. Loprieno, An experimental approach to identify the genotoxic risk from cooked food mutagens, Food Chem. Toxicol. 29 (1991) 377-386.
103. [103] J.D. Tucker, A.V. Carrano, N.A. Allen, M.L. Christensen, M.G. Knize, C.L. Strout, J.S. Felton, In vivo cytogenetic effects of cooked food mutagens, Mutation Res. 224 (1987) 105-113.
104. [104] E. Gebhart, Antimutagens: data and problems, Humangenetik 24 (1974) 1-32.
105. [105] L.R. Ferguson, Antimutagens as cancer preventive agents in the diet, Mutation Res. 307 (1994) 395-410.
106. [106] S. De Flora, C. Ramel, Mechanisms of inhibitors of mutagenesis and carcinogenesis: classification and overview, Mutation Res. 202 (1988) 285-306.
107. [107] S. Knasmüller, F. Kassie, E. Zöhrer, M. Kundi, D. McCoy, R. Schulte Hermann, Effect of ethanol treatment on DNA-damage induced in Escherichia coli in various organs of mice by N-nitrosonornicotine, 4-(methylnitrosamino)-1-(3-pyridil)-1-butanone and N-nitrosopyrrolidine, Carcinogenesis 15 (1994) 263-270.
108. [108] S. Knasmüller, M.D. Friesen, J.A. Holme, J. Alexander, R. Sanyal, F. Kassie, H. Bartsch, Effects of phenethylisothiocyanate on metabolism and on genotoxicity of dimethylnitrosamine and 2-amino-1-methyl-6-phenylimidazo-(4,5-b)-pyridine (PhIP), Mutation Res. 350 (1996) 93-102.
109. [109] T. Ohta, Modification of genotoxicity by naturally occurring flavourings and their derivatives, Crit. Rev. Toxicol. 23 (1993) 127-146.
110. [110] T. Kada, Y. Shirasu, N. Ikegawa, M. Nomoto, Detection of natural bioantimutagens and in vitro and in vivo analysis of their action, in: C. Ramel, B. Lambert, J. Magnussen (Eds.), Genetic Toxicology of Environmental Chemicals, Part A, 1986, pp. 385-393.
111. [111] V.L. Sparnins, P.L. Venegas, L.W. Wattenberg, Glutathione-S-transferase activity: enhancement by compounds inhibiting chemical carcinogenesis by dietary constituents, J. Natl. Cancer Inst. 68 (1982) 493-496.
112. [112] L.W. Wattenberg, L.T.K. Lam, Inhibition of chemical carcinogenesis by phenols, coumarins, aromatic isothiocyanates, flavons and indoles, in: M.S. Zedeck, M. Lipkin (Eds.), Inhibition of Tumor Induction and Development, Plenum, New York, 1981, pp. 1-22.
113. [113] F.J. Wiebel, Do we need in vivo assays in case of negative in vitro results?, in: S. Madle, L. Müller (Eds.), Current Issues in Genetic Toxicology, MMV Press, Munich, 1993, pp. 25-31.
114. [114] V. Daniel, R. Sharon, A. Bensimon, Regulatory elements controlling the basal and drug inducible expression of glutathione-S-transferase Ya subunit gene, DNA 8 (1989) 399-409.
115. [115] V.L. Sparnins, G. Barany, L.W. Wattenberg, Effects from organosulphur compounds from garlic and onions on benzo(a)pyrene-induced neoplasia and glutathione-S-transferase activity in the mouse, Carcinogenesis 9 (1988) 131-134.
116. [116] L.W. Wattenberg, V.L. Sparnins, G. Barany, Inhibition of N-nitrosodiethyl-amine carcinogenesis in mice by naturally occurring organosulphur compounds and monoterpenes, Cancer Res. 49 (1989) 2689-2692.
117. [117] V.A. Gudi, S.V. Singh, Effect of diallylsulfide, a naturally occurring anti-carcinogen on glutathione dependent detoxification enzymes in female CD-1 mouse tissues, Biochem. Pharmacol. 42 (1991) 1261-1265.
118. [118] W.H. Habig, M.J. Pabst, W.B. Jacoby, Glutathione-S-transferases: the first enzymatic step in mercapturic acid formation, J. Biol. Chem. 249 (1974) 7130-7139.
119. [119] M. Emig, A. Reinhard, V. Mersch-Sundermann, A comparative study of five nitro musk compounds for genotoxicity in the SOS chromotest and Salmonella mutagenicity, Toxicol. Lett. 85 (1996) 151-156.
120. [120] V. Mersch-Sundermann, M. Emig, A. Reinhard, Nitro musks are cogenotoxicants by inducing toxifying enzymes in the rat, Mutation Res. 356 (1996) 237-245.