Expression and characterization of the N- and C-terminal ATP-binding domains of MRP1

Biochemical and Biophysical Research Communications

Volumn 273, Issue 3, 2000, Pages 913-919

  Kern, András ^a   Felföldi, Ferenc ^a   Sarkadi, Balázs ^a   Váradi, András ^a  

^a INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

Author keywords

ABC transporters; ATP binding; ATPase activity; Multidrug resistance; Nucleotide binding domains

Indexed keywords

ADENOSINE TRIPHOSPHATASE; ADENOSINE TRIPHOSPHATE; MALTOSE BINDING PROTEIN; MULTIDRUG RESISTANCE PROTEIN; N ETHYLMALEIMIDE;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; ENZYME ACTIVITY; PRIORITY JOURNAL; PROTEIN PURIFICATION;

EID: 0343090423     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2000.3040     Document Type: Article

References (41)

1. Gottesman M. M., Pastan I. Biochemistry of multidrug resistance mediated by the multidrug transporter. Annu. Rev. Biochem. 62:1993;385-427.
2. Zaman G. J., Flens M. J., van Leusden M. R., de Haas M., Mulder H. S., Lankelma J., Pinned H. M., Scheper R. J., Baas F., Broxterman H. J. The human multidrug resistance-associated protein MRP is a plasma membrane drug-efflux pump. Proc. Natl. Acad. Sci. USA. 91:1994;8822-8826.
3. Zaman G. J., Lankelma J., van Tellingen O., Beijnen J., Dekker H., Paulusma C., Oude Elferink R. P., Baas F., Borst P. Role of glutathione in the export of compounds from cells by the multidrug-resistance-associated protein. Proc. Natl. Acad. Sci. USA. 92:1995;7690-7694.
4. Jedlitschky G., Leier I., Buchholz U., Center M., Keppler D. ATP-dependent transport of glutathione S-conjugates by the multidrug resistance-associated protein. Cancer Res. 54:1994;4833-4836.
5. Holló Z., Homolya L., Hegedûs T., Sarkadi B. Transport properties of the multidrug resistance-associated protein. FEBS Lett. 383:1996;99-104.
6. Rao V. V., Dahlheimer J. L., Bardgett M. E., Snyder A. Z., Finch R. A., Sartorelli A. C., Piwnica-Worms D. Choroid plexus epithelial expression of MDR1 P glycoprotein and multidrug resistance-associated protein contribute to the blood-cerebrospinal-fluid drug-permeability barrier. Proc. Natl. Acad. Sci. USA. 96:1999;3900-3905.
7. Wijnholds J., Scheffer G. L., van der Valk M., van der Valk P., Beijnen J. H., Scheper R. J., Borst P. Multidrug resistance protein 1 protects the oropharyngeal mucosal layer and the testicular tubules against drug-induced damage. J. Exp. Med. 188:1998;797-808.
8. Wijnholds J., Evers R., van Leusden M. R., Mol C. A., Zaman G. J., Mayer U., Beijnen J. H., van der Valk M., Krimpenfort P., Borst P. Increased sensitivity to anticancer drugs and decreased inflammatory response in mice lacking the multidrug resistance-associated protein. Nat. Med. 3:1997;1275-1279.
9. Evers R., Zaman G. J., van Deemter L., Jansen H., Calafat J., Oomen L. C., Oude Elferink R. P., Borst P., Schinkel A. H. Basolateral localization and export activity of the human multidrug resistance-associated protein in polarized pig kidney cells. J. Clin. Invest. 97:1996;1211-1218.
10. Walker J. E., Saraste M., Runswick M. J., Gay N. J. Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. EMBO J. 1:1982;945-951.
11. Baubichon-Cortay H., Bagetto L. G., Dayan G., Di Pietro A. Overexpression and purification of the carboxyl-terminal nucleotid binding domain from mouse P-glycoprotein. J. Biol. Chem. 269:1994;22983-22989.
12. Dayan G., Baubichon-Cortay H., Jault J. M., Cortay J. C., Deléage G., Di Pietro A. Recombinant N-terminal nucleotide binding domain from mouse P-glycoprotein. J. Biol. Chem. 271:1996;11652-11658.
13. Sharma S., Rose D. R. Cloning, overexpression, purification, and characterization of the carboxyl-terminal nucleotide binding domain of P-glycoprotein. J. Biol. Chem. 270:1995;14085-14093.
14. Wang C., Castro F. A., Wilkes D. M., Altenberg G. A. Expression and purification of the first nucleotide-binding domain and linker region of human multidrug resistance gene product: comparison of fusions to gluthation S-transferase, thioredoxin and maltose-binding protein. Biochem. J. 338:1999;77-81.
15. Hartman J., Huang Z., Rado T. A., Peng S., Jilling T., Muccio D. D., Sorscher E. J. Recombinant synthesis, purification, and nucleotide binding characteristics of the first nucleotide binding domain of the cystic fibrosis gene product. J. Biol. Chem. 267:1992;6455-6458.
16. Ko Y. H., Thomas P. J., Deleanoy M. R., Pedersen P. L. The cystic fibrosis transmembrane conductance regulator. J. Biol. Chem. 268:1993;24330-24338.
17. Ko Y. H., Pedersen P. L. The first nucleotide binding fold of the cystic fibrosis transmembrane conductance regulator can function as an active ATPase. J. Biol. Chem. 270:1995;22093-22096.
18. Yike I., Ye J., Zhang Y., Manavalan P., Gerken T. A., Dearborn D. G. A recombinant peptide model of the firstnucleotide-binding fold of the cystic fibrosis transmembrane conductance regulator: Comparison of wild-type and delta F508 mutant forms. Protein Sci. 5:1996;89-97.
19. Müller K. M., Ebensperger C., Tampé R. Nucleotide binding to the hydrophilic C-terminal domain of the transporter associated with antigen processing (TAP). J. Biol. Chem. 269:1994;14032-14037.
20. Nikaido K., Liu P.-O., Ames G. F.-L. Purification and characterization of HisP, the ATP-binding subunit of a traffic ATPase (ABC transporter), the histidine permease of Salmonella typhimurium. J. Biol. Chem. 272:1997;27745-27752.
21. Hung L. W., Wang I. X., Nikaido K., Liu P. Q., Ames G. F., Kim S. H. Crystal structure of the ATP-binding subunit of an ABC transporter. Nature. 396:1998;703-707.
22. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
23. Liu R., Sharom F. J. Site-directed fluorescence labeling of P-glycoprotein on cysteine residues in the nucleotide binding domains. Biochemistry. 35:1996;11865-11873.
24. Sarkadi B., Price E. M., Boucher R. C., Germann U. A., Scarborough G. A. Expression of the human multidrug resistance cDNA in insect cells generates a high activity drug-stimulated membrane ATPase. J. Biol. Chem. 267:1992;4854-4858.
25. Hiratsuka T., Uchida K. Preparation and properties of 2′(or 3′)-O-(2,4,6-trinitrophenyl) adenosine 5′-triphosphate, an analog of adenosine triphosphate. BBA. 320:1973;635-647.
26. Hiratsuka T. Fluorescent properties of 2′(or 3′)-O-(2,4,6-trinitrophenyl) adenosine 5′-triphosphate and its use in the study of binding to hevy meromyosin ATPase. BBA. 453:1976;293-297.
27. Bakos É., Evers R., Sinkó E., Váradi A., Borst P., Sarkadi B. Interactions of the human multidrug resistance proteins MRP1 and MRP2 with organic anions. Mol. Pharmacol. 57:2000;760-768.
28. Chang X. B., Hou Z. X., Riordan J. H. Stimulation of ATPase activity of purified multidrug resistance-associated protein by nucleoside diphosphates. J. Biol. Chem. 273:1998;23844-23848.
29. Mao Q., Leslie E. M., Deeley R. G., Cole S. P. C. ATPase activity of purified and reconstituted multidrug resistance protein MRP1 from drug-selected H69AR cells. Biochim. Biophys. Acta. 1461:1999;69-82.
30. Muller M., Bakos E., Welker E., Varadi A., Germann U. A., Gottesman M. M., Morse B. S., Roninson I. B., Sarkadi B. Altered drug-stimulated ATPase activity in mutants of the human multidrug resistance protein. J. Biol. Chem. 271:1996;1877-1883.
31. Szabó K., Welker E., Bakos É., Müller M., Roninson I., Váradi A., Sarkadi B. Drug-stimulated nucleotide trapping in the human multidrug transporter MDR1. J. Biol. Chem. 273:1998;10132-10138.
32. Beaudet L., Urbatsch I. L., Gros P. Mutations in the nucleotide-binding sites of P-glycoprotein that affect substrate specificity modulate substrate-induced adenosine triphosphatase activity. Biochemistry. 37:1998;9073-9082.
33. Al-Shawi M. K., Urbatsch I. L., Senior A. E. Covalent inhibitors of P-glycoprotein ATPase activity. J. Biol. Chem. 269:1994;8986-8992.
34. Loo T. W., Clarke D. M. Covalent modification of human P-glycoprotein mutants containing a single cysteine in either nucleotide-binding fold abolishes drug-stimulated ATPase activity. J. Biol. Chem. 270:1995;22957-22961.
35. Sankaran B., Bhagat S., Senior A. E. Photoaffinity labelling of P-glycoprotein catalytic sites. FEBS Lett. 417:1997;119-122.
36. Senior A. E., Gadsby D. C. ATP hydrolysis cycles and mechanism in P-glycoprotein and CFTR. Semin. Cancer Biol. 8:1997;143-151.
37. Sankaran B., Bhagat S., Senior A. E. Inhibition of P-glycoprotein ATPase activity by procedures involving trapping of nucleotide in catalytic sites. Arch. Biochem. Biophys. 341:1997;160-169.
38. Sankaran B., Bhagat S., Senior A. E. Inhibition of P-glycoprotein ATPase activity by beryllium fluoride. Biochemistry. 36:1997;6847-6853.
39. Gao M., Cui H-R., Loe D. W., Grant C. E., Almquist K. C., Cole S. P. C., Deeley R. G. Comparison of the Functional Characteristics of the Nucleotide Binding Domains of Multidrug Resistance Protein 1. J. Biol. Chem. 275:2000;13098-13108.
40. Hou Y.-X., Cui L., Riordan J. R., Chang X.-B. Allosteric interactions between the two non-equivalent nucleotide binding domains of multidrug resistance protein MRP1. J. Biol. Chem. 2000.
41. Nagata K., Nishitani M., Matsuo M., Kioka N., Amachi T., Ueda K. Non-equivalent nucleotide trapping in the two nucleotide binding folds of the human multidrug resistance protein MRP1. J. Biol. Chem. 2000.