Characterization of a new p94-like calpain form in human lymphocytes

Biochemical Journal

Volumn 375, Issue 3, 2003, Pages 689-696

  De Tullio, Roberta ^a   Stifanese, Roberto ^a   Salamino, Franca ^a   Pontremoli, Sandro ^a   Melloni, Edon ^a  

^a UNIVERSITY OF GENOA   (Italy)

Author keywords

Alternative splicing; Ca2+ dependent proteolysis; Calpain isoform; Circulating white cell

Indexed keywords

BLOOD; CELLS; CYTOLOGY; ENZYMES; IMMUNOLOGY;

ISOFORMS; PERIPHERAL BLOOD MONONUCLEAR CELLS (PBMC);

BIOCHEMISTRY;

CALCIUM ION; CALPAIN; CALPAIN 3; CALPASTATIN; ISOENZYME; LYSINE; PROTEIN; PROTEIN P94; UNCLASSIFIED DRUG;

ARTICLE; B LYMPHOCYTE; CELLULAR IMMUNITY; CONTROLLED STUDY; ENZYME ACTIVATION; EXON; HUMAN; HUMAN CELL; HUMORAL IMMUNITY; IMMUNE RESPONSE; NATURAL KILLER CELL; NUCLEOTIDE SEQUENCE; POLYMORPHONUCLEAR CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; T LYMPHOCYTE;

CALCIUM; CALPAIN; CATALYSIS; CLONING, MOLECULAR; CYSTEINE PROTEINASE INHIBITORS; DNA, COMPLEMENTARY; ENZYME ACTIVATION; ERYTHROCYTES; HUMANS; IMMUNOBLOTTING; ISOENZYMES; LEUPEPTINS; LYMPHOCYTES; NEUTROPHILS; SEQUENCE ANALYSIS, DNA;

INSERTION SEQUENCES;

EID: 0242721312     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20030706     Document Type: Article

References (47)

1. Huang, Y. and Wang, K. K. W. (2001) The calpain family and human disease. Trends Mol. Med. 7, 355-362
2. Strobl, S., Fernandez-Catalan, C., Braun, M., Huber, R., Masumoto, H., Nakagawa, K., Irie, A., Sorimachi, H., Bourenkow, G., Bartunik, H. et al. (2000) The crystal structure of calcium-free human m-calpain suggests an electrostatic switch mechanism for activation by calcium. Proc. Natl. Acad. Sci. U.S.A. 97, 588-592
3. 2+ requirement for activation of μ-and m-calpain. Biochem. J. 367, 263-269
4. Sorimachi, H., Ishiura, S. and Suzuki, K. (1997) Structure and physiological function of calpains. Biochem. J. 328, 721-732
5. Sorimachi, H., Imajoh-Ohmi, S., Emori, Y., Kawasaki, H., Ohno, S., Minami, Y. and Suzuki, K. (1989) Molecular cloning of a novel mammalian calcium-dependent protease distinct from both m-and μ-types. Specific expression of the mRNA in skeletal muscle. J. Biol. Chem. 264, 20106-20111
6. 2+-binding domain. J. Biol. Chem. 268, 19476-19482
7. Nakajima, T., Fukiage, C., Azuma, M., Ma, H. and Shearer, T. R. (2001) Different expression patterns for ubiquitous calpains and Capn3 splice variants in monkey ocular tissues. Biochim. Biophys. Acta 1519, 55-64
8. 2+-activated neutral protease. Biochemistry 27, 8122-8128
9. Reverter, D., Sorimachi, H. and Bode, W. (2001) The structure of calcium-free human m-calpain: implications for calcium activation and function. Trends Cardiovasc. Med. 11, 222-229
10. 2+-requirement and heterodimer stability in m-calpain. J. Biol. Chem. 272, 11268-11275
11. 2+ switch aligns the active site for calpain. Cell. 108, 649-660
12. Reverter, D., Strobl, S., Fernandez-Catalan, C., Sorimachi, H., Suzuki, K. and Bode, W. (2001) Structural basis for possible calcium-induced activation mechanisms of calpains. Biol. Chem. 382, 753-766
13. 2+. J. Biol. Chem. 277, 40296-40301
14. Pal, G. P., Elce, J. S. and Jia, Z. (2001) Dissociation and aggregation of calpain in the presence of calcium. J. Biol. Chem. 276, 47233-47238
15. Suzuki, K. and Sorimachi, H. (1998) A novel aspect of calpain activation. FEBS Lett. 433, 1-4
16. Nakagawa, K., Masumoto, H., Sorimachi, H. and Suzuki, K. (2001) Dissociation of m-calpain subunits occurs after autolysis of the N-terminus of the catalytic subunit and is not required for activation. J. Biochem. (Tokyo) 130, 605-611
17. Wang, K. K. W. (2000) Calpain and caspase: can you tell the difference? Trends Neurosci. 23, 20-26
18. Carragher, N. O. and Frame, M. C. (2002) Calpain: a role in cell transformation and migration. Int. J. Biochem. Cell. Biol. 34, 1539-1543
19. Carafoli, E. and Molinari, M. (1998) Calpain: a protease in search of a function? Biochem. Biophys. Res. Commun. 247, 193-203
20. Lu, T., Xu, Y., Mericle, M. T. and Mellgren, R. L. (2002) Participation of conventional calpains in apoptosis. Biochim. Biophys. Acta 1590, 16-26
21. Azam, M., Andrabi, S. S., Sahr, K. E., Kamath, L., Kuliopulos, A. and Chisti, A. H. (2001) Disruption of the μ-calpain gene reveals an essential role in platelet function. Mol. Cell. Biol. 21, 2213-2220
22. Kinbara, K., Shoichi, I., Tomioka, S., Sorimachi, H., Jeong, S. Y., Amano, S., Kawasaki, H., Kolmerer, B., Kimura, S., Labeit, S. and Suzuki, K. (1998) Purification of native p94, a muscle specific calpain, and characterisation of its autolysis. Biochem. J. 335, 589-596
23. 2+-dependent intramolecular autolysis. FEBS Lett. 532, 401-406
24. Richard, J., Bronx, O., Allamand, V., Fougerousse, F., Chiannilkulchai, N., Bourg, N., Brenguier, L., Devaud, C., Pasturaud, P., Roudaut, C. et al. (1995) Mutations in the proteolytic enzyme calpain 3 causes limb-girdle muscular dystrophy type 2A. Cell 81, 27-40
25. Ono, Y., Shimada, H., Sorimachi, H., Richard, I., Saido, T. C., Beckmann, J. S., Ishiura, S. and Suzuki, K. (1998) Functional defects of a muscle-specific calpain p94 caused by mutations associated with limb-girdle muscular dystrophy type 2A. J. Biol. Chem. 273, 17073-17078
26. Azuma, M., Fukiage, C., Higashine, M., Nakajima, T., Ma, H. and Shearer, T. R. (2000) Identification and characterisation of a retina-specific calpain (Rt88) form rat. Curr. Eye Res. 21, 710-720
27. Ma, H., Fukiage, C., Azuma, M. and Shearer, T. R. (1998) Cloning and expression of mRNA for calpain Lp82 from rat lens: splice variant of p94. Invest. Ophthalmol. Vis. Sci. 39, 454-461
28. Pontremoli, S., Melloni, E., Damiani, G., Salamino, F., Sparatore, B., Michetti, M. and Horecker, B. L. (1988) Effects of a monoclonal anti-calpain antibody on responses of stimulated human neutrophils. Evidence for a role for proteolytically modified protein kinase C. J. Biol. Chem. 263, 1915-1919
29. Averna, M., De Tullio, R., Salamino, F., Melloni, E. and Pontremoli, S. (1999) Phosphorylation of rat brain calpastatins by protein kinase C. FEBS Lett. 450, 13-16
30. Michetti, M., Salamino, F., Tedesco, I., Averna, M., Minafra, R., Melloni, E. and Pontremoli, S. (1996) Autolysis of human erythrocyte calpain produces two active enzyme forms with different cell localisation. FEBS Lett. 392, 11-15
31. Pontremoli, S., Sparatore, B., Salamino, F., Michetti, M., Sacco, O. and Melloni, E. (1985) Reversible activation of human neutrophil calpain promoted by interaction with plasma membranes. Biochem. Int. 11, 35-44
32. Melloni, E., Michetti, M., Salamino, F and Pontremoli, S. (1998) Molecular and functional properties of a calpain activator protein specific for μ-isoforms. J. Biol. Chem. 273, 12827-12831
33. Boyum, A. (1968) Isolation of mononuclear cells and granulocytes from human blood. Scan. J. Clin. Lab. Invest. 21 (suppl. 97), 77-89
34. Beutler, E., West, C. and Bluma, K. G. (1976) The removal of leukocytes and platelets from whole blood. J. Lab. Clin. Med. 88, 328-333
35. Pontremoli, S., Melloni, E., Viotti, P. L., Michetti, M., Salamino, F. and Horecker, B. L. (1991) Identification of two calpastatin forms in rat skeletal muscle and their susceptibility to digestion by homologous calpains. Arch. Biochem. Biophys. 288, 646-652
36. Salamino, F., Averna, M., Tedesco, I., De Tullio, R., Melloni, E. and Pontremoli, S. (1997) Modulation of rat brain calpastatin efficiency by post-translational modifications. FEBS Lett. 412, 433-438
37. Simon, J., Arthur, C., Mykles, D. L. and Donald, L. (2000) Calpain zymography with casein or fluotescein isocyanate casein. In Calpain Methods Protocols (Elce, J. S., ed.), pp. 109-116, Umana Press, Totowa, NJ
38. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227, 680-685
39. Towbin, J., Staehelin, T. and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci U.S.A. 76, 4350-4354
40. Sanger, F., Nicklen, S. and Coulsen, A. R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci U.S.A. 74, 5463-5467
41. Stone, K. L. and Williams, K. (1993) Enzymatic digestion of protein and HPLC peptide isolation. In A Practical Guide to Protein and Peptide Purificaion for Microsequencing, 2nd edn (Matsudaiara, P., ed.), Academic Press. San Diego
42. 2+-dependent proteinase of human erythrocytes. In Calcium and Cell Function, vol. 6 (Cheung, W. Y., ed.), pp. 159-183, Academic Press, Orlando, FL
43. 2+-dependent neutral proteinase of human erythrocytes. Biochem. Biophys. Res. Commun. 129, 389-395
44. 2+-enriched human neuroblastoma LAN-5 cells. FEBS Lett. 475, 17-21
45. Herasse, M., Ono, Y., Fougerousse, F., Kimura, E., Stockholm, D., Beley, C., Montarras, D., Pinset, C., Sorimachi, H., Suzuki, K. et al. (1999) Expression and functional characteristics of calpain 3 isoforms generated through tissue-specific transcriptional and post-transcriptional events. Mol. Cell. Biol. 19, 4047-4055
46. Fukiage, C., Nakajima, E., Ma, H., Azuma, M. and Shearer, T. R. (2002) Characterization and regulation of lens-specific calpain Lp82. J. Biol. Chem. 277, 20678-20685
47. Melloni, E., Michetti, M., Salamino, F., Minafra, R. and Pontremoli, S. (1996) Modulation of the calpain proteolysis by calpastatin and phospholipids. Biochem. Biophys. Res. Commun. 229, 193-197