Identification and characterization of a retina-specific calpain (Rt88) from rat

Current Eye Research

Volumn 21, Issue 3, 2000, Pages 710-720

  Azuma, M ^a   Fukiage, C ^a   Higashine, M ^a   Nakajima, T ^a   Ma, H ^a   Shearer, T R ^a  

^a OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

Author keywords

Calpain p94 isoform; Molecular biology; Proteinases; Rat; Retina

Indexed keywords

CALCIUM; CALPAIN; COMPLEMENTARY DNA; CYSTEINE PROTEINASE; MESSENGER RNA; MUSCLE ENZYME; PROTEIN P94; RECOMBINANT ENZYME; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BACULOVIRUS; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ACTIVATION; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME SYNTHESIS; EXON; IMMUNOBLOTTING; LENS; MOLECULAR CLONING; NONHUMAN; NORTHERN BLOTTING; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FAMILY; RAT; RAT STRAIN; RETINA; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; ZYMOGRAPHY;

EID: 0034536146     PISSN: 02713683     EISSN: None     Source Type: Journal    
DOI: 10.1076/0271-3683(200009)2131-RFT710     Document Type: Article

References (21)

1. Skeletal muscle-specific calpain, p49: Structure and physiological function
2. Calpain inhibition: An overview of its therapeutic potential
3. Identification of a third ubiquitous calpain species - Chicken muscle expresses four distinct calpains
4. Molecular cloning of a novel mammalian calcium-dependent protease distinct from both m- and mu-types. Specific expression of the mRNA in skeletal muscle
5. A novel tissue-specific calpain species expressed predominantly in the stomach comprises two alternative splicing products with and without Ca(2+)-binding domain
6. Cloning and expression of mRNA for calpain Lp82 from rat lens: Splice variant of p94
7. Protein for Lp82 calpain is expressed and enzymatically active in young rat lens
8. Mutations in the proteolytic enzyme calpain 3 cause limb-girdle muscular dystrophy type 2A
9. Functional defects of a muscle-specific calpain, p94, caused by mutations associated with limb-girdle muscular dystrophy type 2A
10. Lp82 calpain during rat lens maturation and cataract formation
11. Molecular cloning and sequence analysis of calpain Rt88 from rat retina/choroid
12. Quantitative RT-PCR for measuring gene expression
13. Casein zymography: A method to study mu-calpain, m-calpain, and their inhibitory agents
14. Structure and physiological function of calpains
15. Expression and functional characteristics of calpain 3 isoforms generated through tissue-specific transcriptional and posttranscriptional events
16. Purification of native p94, a muscle-specific calpain, and characterization of its autolysis
17. Purification and identification of two putative autolytic sites in human calpain 3 (p94) expressed in heterologous system
18. Selective proteolysis of arrestin by calpain. Molecular characteristics and its effect on rhodopsin dephosphorylation
19. Calpain activity in the retinas of normal and RCS rats
20. A point mutation of the rhodopsin gene in one form of retinitis pigmentosa
21. A three-base-pair deletion in the peripherin-RDS gene in one form of retinitis pigmentosa